Search results for "Primer"
showing 10 items of 530 documents
La comprensió de les clàusules de relatiu en català infantil
2021
Les clàusules de relatiu han estat objecte d’un nombre important de treballs en el camp de la gramàtica adulta, la gramàtica infantil i l’afàsia. Aquí emprenen el primer estudi de la comprensió de les clàusules de relatiu de subjecte i d’objecte en català infantil. Repliquem un experiment dissenyat per a l’italià per Adani (2008), en concret una tasca d’identificació d’agent, amb 33 nens parlants del català central d’edats compreses entre els 3,5 i els 6,2 anys. L’anàlisi dels resultats indica que els nens assoleixen nivells adults de comprensió de les relatives de subjecte primerencament, mentre que la comprensió de les relatives d’objecte progressa però no assoleix nivells adults fins a l…
Análisis contextual de la revitalización del euskera en el ámbito familiar
2012
En este trabajo se analiza la revitalización del euskera en el ámbito familiar, situándola en el contexto general de Euskal Herria y en el particular de cada uno de los territorios del euskera. El análisis va desde la mitad del s. xx hasta la actualidad y considera tanto el marco jurídico como la actuación institucional y la dinámica social, siempre con la mirada puesta en los roles desempeñados durante ese tiempo por la familia. La línea argumental está soportada en informaciones y datos sociolingüísticos procedentes de los numerosos estudios e informes, realizados sobre todo a partir de la década de los 80 del siglo pasado. Se concluye con unas reflexiones acerca de las luces y sombras de…
Tabanque : revista pedagógica
2013
Resumen basado en el de la publicación Título, resumen y palabras clave disponibles en español y en inglés La Educación y Atención de la Primera Infancia (EAPI) ha recibido una atención sin precedentes en las esferas políticas y públicas durante los últimos años en el panorama internacional de la educación, y especialmente en las sociedades europeas, como resultado de los hallazgos que la investigación en este campo ha venido demostrando desde tiempo atrás en la historia reciente de la pedagogía infantil y el cuidado de la primera infancia. Desde este hecho, la Unión Europea y la gran mayoría de sus estados miembros han fijado unas metas estratégicas para incrementar la provisión dentro de …
Expression and characterization of the recombinant juvenile hormone epoxide hydrolase (JHEH) from Manduca sexta.
1998
The cDNA of the microsomal Juvenile Hormone Epoxide Hydrolase (JHEH) from Manduca sexta was expressed in vitro in the baculovirus system. In insect cell culture, the recombinant enzyme (Ms-JHEH) was produced at a high level (100 fold over background EH catalytic activity). As expected, Ms-JHEH was localized in the microsomal fraction with a molecular mass of approximately 50 kDa. Ms-JHEH showed a substrate and inhibitor spectrum similar to the wild type JHEH isolated from eggs of M. sexta. Its enzymatic activity was the highest for Juvenile Hormone III. Ms-JHEH hydrolyzed several trans-epoxides faster than cis-epoxides. A putative hydroxyl-acyl enzyme intermediate was isolated suggesting a …
Synthesis of nucleoside and nucleotide conjugates of bile acids, and polymerase construction of bile acid-functionalized DNA.
2010
Aqueous Sonogashira cross-coupling reactions of 5-iodopyrimidine or 7-iodo-7-deazaadenine nucleosides with bile acid-derived terminal acetylenes linked via an ester or amide tether gave the corresponding bile acid–nucleoside conjugates. Analogous reactions of halogenated nucleoside triphosphates gave directly bile acid-modified dNTPs. Enzymatic incorporation of these modified nucleotides to DNA was successfully performed using Phusion polymerase for primer extension. One of the dNTPs (dCTP bearing cholic acid) was also efficient for PCR amplification.
Domains of the E1 Protein of Human Papillomavirus Type 33 Involved in Binding to the E2 Protein
1996
Papillomavirus E1 and E2 proteins are essential for the initiation of viral DNA replication. We have now analyzed the interaction of E1 and E2 of human papillomavirus type 33, which is associated with cervical carcinoma. When synthesized in insect cells using the baculovirus expression system, the E1 and E2 proteins interacted efficiently at 4 degree. A monoclonal antibody recognizing E1 amino acids 584--600 inhibited the binding of E2 and vice versa, indicating that these amino acids are involved in E2 binding. To confirm this result, a mutational analysis of E1 was performed. The E2 binding activity of E1 deletion and point mutant proteins was assayed using glutathione S-transferase E1 fu…
Effects of endocrine disruptors on genes associated with 17 beta-estradiol metabolism and excretion
2008
International audience; In order to provide a global analysis of the effects of endocrine disruptors on the hormone cellular bioavailability, we combined 17 beta-estradiol (E2) cellular flow studies with real-time PCR and Western blot expression measurements of genes involved in the hormone metabolism and excretion. Three endocrine disruptors commonly found in food were chosen for this study, which was conducted in the estrogen receptor (ER) negative hepatoblastoma HepG2 cell line: bisphenol A (BPA), genistein (GEN) and resveratrol (RES). We showed that 24h after a single dose treatment with genistein, resveratrol or bisphenol A, the expression of ATP-binding cassette transporters (the mult…
Aromatase and amphiregulin are correspondingly expressed in human liver cancer cells
2009
Human hepatocellular carcinoma (HCC) is associated with high mortality rates, being the third most common cause of cancer death worldwide. Although estrogens have been implicated in HCC, their potential role in development and/or progression of this malignancy remains unclear. In this study we investigated mRNA and protein expression of aromatase (Aro) and amphiregulin (AREG) in relation to estrogen receptors (ERs), in HepG2, Huh7, and HA22T human malignant liver cell lines, using RT-PCR and Western blot analyses. Aro expression was significantly higher (approximately 13-fold, P= 0.003) in HepG2 cells than in Huh7 cells, while no Aro expression could be detected in HA22T cells. Interestingl…
Association between CCK-AR gene and schizophrenia with auditory hallucinations
2007
[Objective]: Previous studies on a possible association between CCK-AR polymorphisms and schizophrenia have been controversial. The aim of the present study was to assess a potential association between schizophrenic patients with auditory hallucinations and polymorphisms of the CCK-AR gene.
Progressive Endoplasmic Reticulum Stress Contributes to Hepatocarcinogenesis in Fatty Acyl-CoA Oxidase 1–Deficient Mice
2011
Fatty acyl-coenzyme A oxidase 1 (ACOX1) knockout (ACOX1(-/-)) mice manifest hepatic metabolic derangements that lead to the development of steatohepatitis, hepatocellular regeneration, spontaneous peroxisome proliferation, and hepatocellular carcinomas. Deficiency of ACOX1 results in unmetabolized substrates of this enzyme that function as biological ligands for peroxisome proliferator-activated receptor-α (PPARα) in liver. Here we demonstrate that sustained activation of PPARα in ACOX1(-/-) mouse liver by these ACOX1 substrates results in endoplasmic reticulum (ER) stress. Overexpression of transcriptional regulator p8 and its ER stress-related effectors such as the pseudokinase tribbles h…