Search results for "Printing"
showing 10 items of 532 documents
Multiple Surface Cracking and Debonding Failure for Thin Thermal Coatings
2019
Abstract A mechanical analysis of thin films of quasi-brittle materials used as thermal coatings for superalloy substrate is proposed. The study considers a bi-material element subjected to uniform tension formed by a thin layer of quasi-brittle material (typically a ceramic) bonded on an elastic substrate. The bounding between the coating film and the substrate is realized by a very thin primer which mechanically modeled as a zero thickness cohesive frictional interface. The analysis is developed by a non-linear finite element simulation in which, in order to consider damage size effects, a non-local isotropic damage model is adopted for the quasi-brittle coating. The results of the analys…
Proactive design and formalization of related spatiotemporal knowledge : application to assembly process and additive manufacturing with smart materi…
2019
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Intégration proactive des métiers en conception et formalisation des connaissances spatio-temporelles associées : application à l'assemblage et à la …
2019
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Biomolecular conjugation inside synthetic polymer nanopores via glycoprotein-lectin interactions
2011
We demonstrate the supramolecular bioconjugation of concanavalin A (Con A) protein with glycoenzyme horseradish peroxidase (HRP) inside single nanopores, fabricated in heavy ion tracked polymer membranes. Firstly, the HRP-enzyme was covalently immobilized on the inner wall of the pores using carbodiimide coupling chemistry. The immobilized HRP-enzyme molecules bear sugar (mannose) groups available for the binding of Con A protein. Secondly, the bioconjugation of Con A on the pore wall was achieved through its biospecific interactions with the mannose residues of the HRP enzyme. The immobilization of biomolecules inside the nanopore leads to the reduction of the available area for ionic tran…
Biotemplated Lithography of Inorganic Nanostructures (BLIN) for Versatile Patterning of Functional Materials
2020
Here, we present a highly parallel fabrication method dubbed biotemplated lithography of inorganic nanostructures (BLIN) that enables large-scale versatile substrate patterning of metallic and semi...
Analysis of Nicotine and Its Oxidation Products in Nicotine Chewing Gum by a Molecularly Imprinted Solid-Phase Extraction
2000
Chromatographic stationary phases showing exceptional selectivity for nicotine can be prepared by the technique of molecular imprinting. Such phases were used in the search for a rapid cleanup step for nicotine and some of its oxidation products in chewing gum formulations. Thus, using an organic mobile phase, the nicotine analytes from chewing gums dissolved in nonpolar solvent were retained, whereas the nonpolar matrix eluted close to the void peak. A subsequent switch to an acidic mobile phase resulted in elution of the analytes as one sharp peak. Due to weak binding of the less basic oxidation products, other imprinted polymers were tested, and the solid-phase extraction procedure was o…
Molecular analysis of the nitrate-reducing community from unplanted and maize-planted soils
2002
ABSTRACT Microorganisms that use nitrate as an alternative terminal electron acceptor play an important role in the global nitrogen cycle. The diversity of the nitrate-reducing community in soil and the influence of the maize roots on the structure of this community were studied. The narG gene encoding the membrane bound nitrate reductase was selected as a functional marker for the nitrate-reducing community. The use of narG is of special interest because the phylogeny of the narG gene closely reflects the 16S ribosomal DNA phylogeny. Therefore, targeting the narG gene provided for the first time a unique insight into the taxonomic composition of the nitrate-reducing community in planted an…
Low-cost method for obtaining medical rapid prototyping using desktop 3d printing : a novel technique for mandibular reconstruction planning
2017
Background Three-dimensional (3D) printing is relatively a new technology with clinical applications, which enable us to create rapid accurate prototype of the selected anatomic region, making it possible to plan complex surgery and pre-bend hardware for individual surgical cases. This study aimed to express our experience with the use of medical rapid prototype (MRP) of the maxillofacial region created by desktop 3D printer and its application in maxillofacial reconstructive surgeries. Material and methods Three patients with benign mandible tumors were included in this study after obtaining informed consent. All patient's maxillofacial CT scan data was processed by segmentation and isolat…
Development of micro-contact printing of osteosarcoma cells using MeV ion beam lithography
2009
Abstract For investigation of spatial effects in signalling between cells and also signal substances that trigger cell proliferation and behaviour we are developing a micro Contact Printing process ( μ CP ) . In order to allow printing of cells stamps with high aspect ratio are required and these have been fabricated using Programmed Proximity Aperture Lithography (PPAL) with 3 MeV 4 He 2 + ions to produce PMMA masters for casting the stamps in PDMS. A simple printing device was developed and the first results using this to print human osteosarcoma cells is demonstrated.
A compact linear Paul trap cooler buncher for CRIS
2020
A gas-filled linear Paul trap for the Collinear Resonance Ionisation Spectroscopy (CRIS) experiment at ISOLDE, CERN is currently under development. The trap is designed to accept beam from both ISOLDE target stations and the CRIS stable ion source. The motivation for the project along with the current design, simulations and future plans, will be outlined. peerReviewed