Search results for "Probe"
showing 10 items of 534 documents
Photoinduced Electron Transfer in a Porphyrin-Fullerene Dyad at a Liquid Interface
2022
The excited-state properties of an amphiphilic porphyrin–fullerene dyad and of its porphyrin analogue adsorbed at the dodecane/water interface are investigated by using surface second-harmonic generation. Although the porphyrin is formally centrosymmetric, the second-harmonic spectra of both compounds are dominated by the intense Soret band of the porphyrin. Polarization-selective measurements and molecular dynamics simulations suggest an angle of about 45° between the donor–acceptor axis and the interfacial plane, with the porphyrin interacting mostly with the nonpolar phase. Time-resolved measurements reveal a marked concentration dependence of the dynamics of both compounds upon Q-band e…
High-energy resummation effects in the production of Mueller-Navelet dijet at the LHC
2016
We study the production of two forward jets with a large interval of rapidity at hadron colliders, which was proposed by Mueller and Navelet as a possible test of the high energy dynamics of QCD, within a complete next-to-leading logarithm framework. We show that using the Brodsky-Lepage-Mackenzie procedure to fix the renormalization scale leads to a very good description of the recent CMS data at the LHC for the azimuthal correlations of the jets. We show that the inclusion of next-to-leading order corrections to the jet vertex significantly reduces the importance of energy-momentum non-conservation which is inherent to the BFKL approach, for an asymmetric jet configuration. Finally, we ar…
Optimizing the SYBR green related cyanine dye structure to aim for brighter nucleic acid visualization
2022
In recent years, the studies of RNA and its use for the development of RNA based vaccines have increased drastically. Although cyanine dyes are commonly used probes for studying nucleic acids, in a wide range of applications, there is still a growing need for better and brighter dyes. To meet this demand, we have systematically studied the structure of SYBR green-related cyanine dyes to gain a deeper understanding of their interactions with biomolecules especially how they interact with nucleic acids and the structural components which makes them strongly fluorescent. Herein, five new dyes were synthesized, and their photophysical properties were evaluated. Observations of photophysical cha…
Mung Bean nuclease mapping of RNAs 3' end
2009
Abstract A method is described that allows an accurate mapping of 3' ends of RNAs. In this method a labeled DNA probe, containing the presumed 3' end of the RNA under analysis is allowed to anneals to the RNA itself. Mung-bean nuclease is then used to digest single strands of both RNA and DNA. Electrophoretic fractionation of "protected" undigested, labeled DNA is than performed using a sequence reaction of a known DNA as length marker. This procedure was applied to the analysis of both a polyA RNA (Interleukin 10 mRNA) and non polyA RNAs (sea urchin 18S and 26S rRNAs). This method might be potentially relevant for the evaluation of the role of posttrascriptional control of IL-10 in the pat…
Thermodynamic correction of particle concentrations measured by underwing probes on fast-flying aircraft
2016
Particle concentration measurements with underwing probes on aircraft are impacted by air compression upstream of the instrument body as a function of flight velocity. In particular, for fast-flying aircraft the necessity arises to account for compression of the air sample volume. Hence, a correction procedure is needed to invert measured particle number concentrations to ambient conditions that is commonly applicable to different instruments to gain comparable results. In the compression region where the detection of particles occurs (i.e. under factual measurement conditions), pressure and temperature of the air sample are increased compared to ambient (undisturbed) conditions in certain …
Application of Tuning Fork Sensors for In-situ Studies of Dynamic Force Interactions Inside Scanning and Transmission Electron Microscopes
2012
Mechanical properties of nanoscale contacts have been probed in-situ by specially developed force sensor based on a quartz tuning fork resonator (TF). Additional control is provided by observation of process in scanning electron microscope (SEM) and transmission electron microscope (TEM). A piezoelectric manipulator allows precise positioning of atomic force microscope (AFM) probe in contact with another electrode and recording of the TF oscillation amplitude and phase while simultaneously visualizing the contact area in electron microscope. Electrostatic control of interaction between the electrodes is demonstrated during observation of the experiment in SEM. In the TEM system the TF senso…
Impact of Vanadium Complexes with Tetradentate Schiff Base Ligands on the DPPC and EYL Liposome Membranes: EPR Studies
2023
This paper investigates the effect of three vanadium complexes with Schiff base-type tetradentate ligands of general formula N,N′-1,2-cyclohexylenebis(3,5-dichlorosalicylideneiminate) (V1); LVCl2 (L = N,N′-1,2-cyclohexylenebis(5-chlorosalicylideneiminate) (V2); and N,N′-1,3propylenebis (salicylideneiminate) (V3) on the fluidity of liposome membranes obtained by the sonication of natural lecithin (EYL) and synthetic lecithin (DPPC). The study was carried out with TEMPO and 16DOXYL spin probes using the EPR technique. The results show that the effect of the complexes on the fluidity of liposomes whose membranes are in the liquid crystalline phase is much stronger as compared to the liposome m…
Molecular cloning and characterization of the complementary DNA coding for the B-chain of murine Clq
1989
AbstractcDNA clones coding for the B-chain of murine Clq were isolated from a mouse macrophage library. The characterized clones include the total coding region plus a leader sequence. High homology was found with human Clq B-chain in the coding region (81%). Northern blot analysis of total RNA from different tissues of Balb/c mice showed one band of approximately 1.2 kb. The highest signal was found in RNA preparations of thioglycolate-activated peritoneal macrophages. The probe also hybridized with mRNA from spleen, thymus and heart. Extremely weak signals were found in liver, kidney, lung and intestine tissues.
Clinical, Microscopic and Ultrastructural Findings in a Case of Short Anagen Syndrome
2017
Short anagen syndrome is an uncommon and recently described disease characterized by many telogen hairs and short maximum hair length. We report here the case of a 3-year-old girl whose short, sparse, fine hair since birth was consistent with short anagen syndrome. X-ray microanalysis demonstrated normal composition of the main bioelements of her hairs.
Distribution of type I and type II collagen gene expression during the development of human long bones
1990
The temporal and spatial gene expression of collagen type I and type II during the development of the human long bones was studied by the technique of in situ hybridization covering the period from the cartilagenous bone anlage to the formation of a regular growth plate in the newborn. Analysis of the early stages around the seventh week of gestation revealed for type II collagen a strong hybridization signal limited to the chondrogenic tissue. The surrounding connective tissue and the perichondrium showed weak type I collagen expression, while the zones of desmal ossification like the clavicle gave a strong signal. Beginning with the eighth week of gestation, type I collagen mRNA was detec…