Search results for "Processes"
showing 10 items of 3831 documents
The Information Society and the New Competence
1988
El patrimonio y paisaje del agua de l’Horta Sud como recursos para una propuesta didáctica
2021
La Huerta de Valencia, paisaje cultural milenario, con valores ambientales, históricos, económicos, culturales y simbólicos, se presenta como un magnífico escenario sobre el que desplegar una propuesta didáctica. Una propuesta que apueste por el aprovechamiento del patrimonio cultural y paisajístico como recurso educativo. Existe toda una línea de investigación en torno al potencial educativo del patrimonio cultural y del paisaje. Tras su revisión, y tras cartografiar, inventariar y analizar los bienes patrimoniales del área de estudio, se plantea como objetivo el diseño de una propuesta didáctica articulada en torno a un itinerario por la Huerta, como estrategia de aprendizaje integral par…
The Proteome and Lipidome of Synechocystis sp. PCC 6803 Cells Grown under Light-Activated Heterotrophic Conditions*
2015
Cyanobacteria are photoautotrophic prokaryotes with a plant-like photosynthetic machinery. Because of their short generation times, the ease of their genetic manipulation, and the limited size of their genome and proteome, cyanobacteria are popular model organisms for photosynthetic research. Although the principal mechanisms of photosynthesis are well-known, much less is known about the biogenesis of the thylakoid membrane, hosting the components of the photosynthetic, and respiratory electron transport chain in cyanobacteria. Here we present a detailed proteome analysis of the important model and host organism Synechocystis sp. PCC 6803 under light-activated heterotrophic growth condition…
Interaction with OGG1 Is Required for Efficient Recruitment of XRCC1 to Base Excision Repair and Maintenance of Genetic Stability after Exposure to O…
2015
International audience; XRCC1 is an essential protein required for the maintenance of genomic stability through its implication in DNA repair. The main function of XRCC1 is associated with its role in the single-strand break (SSB) and base excision repair (BER) pathways that share several enzymatic steps. We show here that the polymorphic XRCC1 variant R194W presents a defect in its interaction with the DNA glycosylase OGG1 after oxidative stress. While proficient for single-strand break repair (SSBR), this variant does not colocalize with OGG1, reflecting a defect in its involvement in BER. Consistent with a role of XRCC1 in the coordination of the BER pathway, induction of oxidative base …
Photogenotoxicity of folic acid.
2013
Folic acid (FA), also named vitamin B9, is an essential cofactor for the synthesis of DNA bases and other biomolecules after bioactivation by dihydrofolate reductase (DHFR). FA is photoreactive and has been shown to generate DNA modifications when irradiated with UVA (360 nm) in the presence of DNA under cell-free conditions. To investigate the relevance of this reaction for cells and tissues, we irradiated three different cell lines (KB nasopharyngeal carcinoma cells, HaCaT keratinocytes, and a melanoma cell line) in the presence of FA and quantified cytotoxicity and DNA damage generation. The results indicate that FA is phototoxic and photogenotoxic by two different mechanisms. First, ext…
Heterotrophic microorganisms in deteriorated medieval wall paintings in southern Italian churches
2008
The Campania region in southern Italy is noted for its large number of churches that harbour invaluable frescoes, dated from the beginnings of the 4th up to the 13th century. The wall paintings represent an integral part of the monuments, and their deterioration constitutes a potentially significant loss for the world's cultural heritage. Heterotrophic microorganisms such as bacteria and mould can grow on the surface of paintings that contain a wide range of organic and inorganic constituents, and provide different ecological. niches that are exploited by a large variety of microbial. species. We isolated and identified the heterotrophic microorganisms found in the biodegraded medieval wall…
Diversity and distribution of marine heterotrophic bacteria from a large culture collection
2020
16 pages, 5 figures, 3 tables, supplementary information https://doi.org/10.1186/s12866-020-01884-7
Cloning and sequencing of the dnaK region of Streptomyces coelicolor A3(2)
1993
Abstract The dnaK homologue of Streptomyces coelicolor A3(2) strain M145 has been cloned and sequenced. Nucleotide sequence analysis of a 2.5-kb region revealed an open reading frame (ORF) encoding a predicted DnaK protein of 618 amino acids (Mr = 66 274). The dnaK coding sequence displays extreme codon bias and shows a strong preference for CGY and GGY, for Arg and Gly codons, respectively. The predicted DnaK sequence has a high Lys:Arg ratio which is not typical of streptomycete proteins. The region immediately downstream from dnaK contains an ORF for a GrpE-like protein; the predicted start codon of grpE overlaps the last two codons of dnaK, indicating that the two genes are translationa…
Interaction between turbulent structures and particles in roughened channel
2016
Abstract The distribution of inertial particles in turbulent flows is highly non-uniform and is driven by the local dynamics of the turbulent structures of the underlying carrier flow field. In the specific context of dilute particle-laden wall-bounded flows, deposition and resuspension mechanisms are dominated by the interaction between inertial particles and coherent turbulent structures characteristic of the wall region. The macroscopic behavior of these two-phase systems is influenced by particle inertia, which plays a role at the microscale of a single dispersed element. These turbulent structures, which control the turbulent regeneration cycles, are strongly affected by the wall rough…
Integrating fire-scar, charcoal and fungal spore data to study fire events in the boreal forest of northern Europe
2019
Fire is a major disturbance agent in the boreal forest, influencing many current and future ecosystem conditions and services. Surprisingly few studies have attempted to improve the accuracy of fire-event reconstructions even though the estimates of the occurrence of past fires may be biased, influencing the reliability of the models employing those data (e.g. C stock, cycle). This study aimed to demonstrate how three types of fire proxies – fire scars from tree rings, sedimentary charcoal and, for the first time in this context, fungal spores of Neurospora – can be integrated to achieve a better understanding of past fire dynamics. By studying charcoal and Neurospora from sediment cores f…