6533b7cffe1ef96bd1259160
RESEARCH PRODUCT
Cloning and sequencing of the dnaK region of Streptomyces coelicolor A3(2)
Rosa PassantinoColin P. SmithMarcella AlbertiGiselda BuccaGregorio SeiditaAnna Maria Pugliasubject
DNA BacterialMolecular Sequence Datagenetic processesBacterial ProteinsStart codonGeneticsCoding regionHSP70 Heat-Shock ProteinsAmino Acid SequenceCloning MolecularCodonGeneHeat-Shock Proteinschemistry.chemical_classificationGeneticsBase SequencebiologyEscherichia coli ProteinsStreptomyces coelicolorNucleic acid sequenceStreptococcusGeneral Medicinebiology.organism_classificationAmino acidOpen reading framechemistryGenes BacterialProtein BiosynthesisCodon usage biasbiological sciencesbacteriaSequence Alignmentdescription
Abstract The dnaK homologue of Streptomyces coelicolor A3(2) strain M145 has been cloned and sequenced. Nucleotide sequence analysis of a 2.5-kb region revealed an open reading frame (ORF) encoding a predicted DnaK protein of 618 amino acids (Mr = 66 274). The dnaK coding sequence displays extreme codon bias and shows a strong preference for CGY and GGY, for Arg and Gly codons, respectively. The predicted DnaK sequence has a high Lys:Arg ratio which is not typical of streptomycete proteins. The region immediately downstream from dnaK contains an ORF for a GrpE-like protein; the predicted start codon of grpE overlaps the last two codons of dnaK, indicating that the two genes are translationally coupled. This organisation diners from that reported for other prokaryotes.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1993-08-16 | Gene |