Search results for "Production"
showing 10 items of 7600 documents
THE SMALL PROTEIN TRPM MODULATES MORPHO-PHYSIOLOGICAL DIFFERENTIATION IN THE MODEL ACTINOMYCETE STREPTOMYCES COELICOLOR A3(2)
2017
BACKGROUNDS TrpM, a small protein of 63 amino acids, modulates tryptophan (Trp) metabolism and morpho-physiological differentiation in the filamentous bacterium Streptomyces coelicolor A3(2), a model organism for antibiotic production and cell differentiation. Indeed, the trpM knock-out mutant strain is characterized by a delayed growth on minimal medium, smaller aerial hyphae, and reduction of both spore and antibiotic actinorhodin production in comparison with the wild-type strain. These observations were in agreement with proteomic analyses which highlighted a role for TrpM in controlling i) Trp production through Trp precursor availability and, thus ii) bacterial growth and morpho-physi…
The small protein TrpM modulates morpho-physiological differentiation in Streptomyces coelicolor
2017
TrpM, a small protein of 63 amino acids, is encoded by a gene of the trpCMBA locus involved in tryptophan biosynthesis in the model actinomycete Streptomyces coelicolor. Indeed, the trpM knock-out mutant strain is characterized by a delayed growth on minimal medium, smaller aerial hyphae, and reduction of both spore and antibiotic actinorhodin production in comparison with the wild-type strain. These observations are in agreement with proteomic analyses which highlighted a role for TrpM in controlling i) tryptophan production through precursor availability and, thus ii) bacterial growth and morpho-physiological differentiation. To further elucidate the role of TrpM, a S. coelicolor trpM kno…
A small protein is involved in tryptophan biosynthesis and morpho-physiological differentiation in Streptomyces coelicolor
2015
A small protein is involved in tryptophan biosynthesis and morpho-physiological differentiation in Streptomyces coelicolor
From tryptophan metabolism to peptide antibiotic production in Streptomyces coelicolor
2009
The tryptophan is a precursor of the lipopeptide calcium dependent antibiotic (CDA), produced from Streptomyces coelicolor and closely related to important antibiotics such as daptomycin. We have focused our attention on the correlation between CDA production and tryptophan metabolism in order to identify new strategies aimed at increasing the production of peptide antibiotics.
Streptomyces coelicolor secretoma protects astrocytes from oxidative stress
2016
Streptomycetes are gram-positive bacteria that produce about 2/3 of pharmaceutically active secondary metabolites, such as antibiotics, and anti-tumor, immunosuppressive, antifungal and antiparasitic agents. In this study, we investigated the possible effects of Streptomyces coelicolor extracts and putative vesicular fraction on primary cultures of rat astrocytes, in both physiological and stressed conditions, induced by treatment with hydrogen peroxide. Briefly, crude extracts and putative vesicular fractions were prepared from two S.coelicolor strains (M145 wild-type strain and bold F166 strain), and used to treat primary astrocytes, which were then also treated with hydrogen peroxide. Da…
CyProF – Insights from a Framework for Designing Cyber-Physical Systems in Production Environments
2016
Abstract There is little experience in integrating cyber-physical systems (CPS) in production environments nowadays. A coordinated framework with reference architecture, methodology and procedural model can support the adaption of CPS by providing a pre-defined structure and guidelines. This paper describes such a framework, named CyProF, for designing CPS-based solutions in production environments. Engineers are supported by this framework in handling the complexity during the design phase. Additionally, this paper explains how the framework was used in a use case to reduce engineering efforts for a CPS-based demonstrator.
Solving the Problems of Inspection Planning under Parametric Uncertainty of Underlying Models
2013
Certain fatigued structures must be inspected in order to detect fatigue damages that would otherwise not be apparent. A technique for obtaining optimal inspection strategies is proposed for situations where it is difficult to quantify the costs associated with inspections and undetected failure. For fatigued structures for which failures (fatigue damages) are only detected at the time of inspection, it is important to be able to determine the optimal times of inspection. Fewer inspections will lead to lower fatigue reliability of the structure upon demand, and frequent inspection will lead to higher cost. When there is a fatigue reliability requirement, the problem is usually to develop an…
Organization of Production Processes through the Multi-Service Method by Performing some Identical Operations of the same Duration and Structure
2013
Organisation of production processes, by means of multi service method refers to the performance of attributions within a working system, using simultaneously or successively more inputs or more items in a single means of production, by one or more employees. In the specifically chosen case, in order to outline the advantages that shall be obtained after implementing the multi service method, we want to illustrate a situation where there are the execution conditions of certain identical operations, with the same duration and structure, in which case the running time of the performer and the means of production will be identical. Serving multiple machines finds application in all industrial …
A Potted History: Spain 1860–2015
2018
This chapter aims to supply a synthetic view of the historical process of economic development in Spain broken down into major stages. Rather than examine it from all angles, the text concentrates on changes involving the elements highlighted in economic theory and empirical works, that is, the rate at which the Spanish economy advanced, the evolution of its production structure and sector employment, and the degree of integration of the goods and factor markets and the effects of this in both national and supranational terms. So as to give a stylized view of the process, the analysis is carried out on the basis of four major stages into which we divide the period of study: 1860–1910, 1910–…
Firefly algorithm based upon slicing structure encoding for unequal facility layout problem
2019
Finding the locations of departments or machines in a workspace is classified as a Facility Layout Problem. Good placement of departments has a relevant influence on manufacturing costs, work in process, lead times and production efficiency. This paper analyses the problem of allocating departments with restrictions in terms of unequal area and rectangular shape within a facility, in order to minimize the sum of material handling costs taking into account the satisfaction of the aspect ratio requested. In particular, we propose for the first time a Firefly Algorithm based on the slicing structure encoding. The proposed method was tested comparing the results obtained from other authors on t…