Search results for "Programming"

MYC-driven epigenetic reprogramming favors the onset of tumorigensis by inducing a stem cell-like state

2017

AbstractBreast cancer consists of highly heterogeneous tumors, whose cell of origin and driver oncogenes resulted difficult to be uniquely defined. Here we report that MYC acts as tumor reprogramming factor in mammary epithelial cells by inducing an alternative epigenetic program, which triggers loss of cell identity and activation of oncogenic pathways. Over-expression of MYC induces transcriptional repression of lineage-specifying transcription factors, causing decommissioning of luminal-specific enhancers. MYC-driven dedifferentiation supports the onset of a stem cell-like state by inducing the activation of de novo enhancers, which drive the transcriptional activation of oncogenic pathw…

Pericytes: a target for in vivo reprogramming?

2013

Identical fits of nonnegative matrix/tensor factorization may correspond to different extracted event-related potentials

2010

Nonnegative Matrix / Tensor factorization (NMF/NTF) have been used in the study of EEG, and the fit (explained variation) is often used to evaluate the performance of a nonnegative decomposition algorithm. However, this parameter only reveals the information derived from the mathematical model and just exhibits the reliability of the algorithms, and the property of EEG can not be reflected. If fits of two algorithms are identical, it is necessary to examine whether the desired components extracted by them are identical too. In order to verify this doubt, we performed NMF and NTF on the same dataset of an auditory event-related potentials (ERPs), and found that the identical fits of NMF and …

Tips and tricks for flow cytometry-based analysis and counting of microparticles

2015

Submicron-sized extra-cellular vesicles generated by budding from the external cell membranes, microparticles (MPs) are important actors in transfusion as well as in other medical specialties. After briefly positioning their role in the characterization of labile blood products, this technically oriented chapter aims to review practical points that need to be considered when trying to use flow cytometry for the analysis, characterization and absolute counting of MP subsets. Subjects of active discussions relative to instrumentation will include the choice of the trigger parameter, possible standardization approaches requiring instrument quality-control, origin and control of non-specific ba…

The number of contacts in random fibre networks

2012

There is a wide range of materials that can be considered as nonwoven random networks of fibres. Such materials include glass-fibre mats, filters, various paper products and structural components of cells and tissues. The mechanical properties of these kinds of networks have been studied extensively for many decades. As many of such networks form more or less two-dimensional structures, they can, to a good approximation, be considered to consist of randomly distributed fibres or filaments connected at their crossings points. Recent development of the resolution of X-ray computed tomography have enabled imaging of the three dimensional structure of such materials with a resolution sufficient…

Classification of Schizophrenia Patients and Healthy Controls Using ICA of Complex-Valued fMRI Data and Convolutional Neural Networks

2019

Deep learning has contributed greatly to functional magnetic resonance imaging (fMRI) analysis, however, spatial maps derived from fMRI data by independent component analysis (ICA), as promising biomarkers, have rarely been directly used to perform individualized diagnosis. As such, this study proposes a novel framework combining ICA and convolutional neural network (CNN) for classifying schizophrenia patients (SZs) and healthy controls (HCs). ICA is first used to obtain components of interest which have been previously implicated in schizophrenia. Functionally informative slices of these components are then selected and labelled. CNN is finally employed to learn hierarchical diagnostic fea…

On the Use of XML in Medical Imaging Web-Based Applications

2016

The rapid growth of digital technology in medical fields over recent years has increased the need for applications able to manage patient medical records, imaging data, and chart information. Web-based applications are implemented with the purpose to link digital databases, storage and transmission protocols, management of large volumes of data and security concepts, allowing the possibility to read, analyze, and even diagnose remotely from the medical center where the information was acquired. The objective of this paper is to analyze the use of the Extensible Markup Language (XML) language in web-based applications that aid in diagnosis or treatment of patients, considering how this proto…

Ambulatory blood pressure monitoring is ready to replace clinic blood pressure in the diagnosis of hypertension: con side of the argument.

2014

The San Francisco experience with Perloff and Sokolow was the starting point for the clinical application of ambulatory blood pressure (ABP) measurement. Using a semiautomatic device, the superiority of ABP to office measurement was demonstrated in the relationship with hypertension-induced organ damage1 and in the risk for cardiovascular events.2 This seminal study impelled an issue with the largest production and impact in the field of hypertension in recent decades, boosting research and having an enormous influence on daily clinical practice. Initially restricted to specialized clinics, ABP monitoring (ABPM) has largely expanded to primary care in many countries. Similarly, scientific p…

Food as medicine in psychiatric care: Which profession should be responsible for imparting knowledge and use of omega-3 fatty acids in psychiatry

2011

Accepted version of an article in Complementary Therapies in Clinical Practice, 17 (2011), 107-112. Also available from the publisher: http://dx.doi.org/10.1016/j.ctcp.2010.08.001 The effect of omega-3 fatty acids on depression is well documented. The purpose of this study was to determine if and how food is used as medicine in psychiatric care, especially how omega-3 fatty acids are used as a supplement in the treatment of depression. This is a pilot study with a qualitative design using questionnaires and interviews among nursing students, tutor nurses and psychiatrists. Three main categories emerged: 1. Nutrition is considered important but few evaluations are made. 2. There was a lack o…

In vitro reprogramming of pancreatic alpha cells towards a beta cell phenotype following ectopic HNF4α expression

2015

There is currently a shortage of organ donors available for pancreatic beta cell transplantation into diabetic patients. An alternative source of beta cells is pre-existing pancreatic cells. While we know that beta cells can arise directly from alpha cells during pancreatic regeneration we do not understand the molecular basis for the switch in phenotype. The aim of the present study was to investigate if hepatocyte nuclear factor 4 alpha (HNF4α), a transcription factor essential for a normal beta cell phenotype, could induce the reprogramming of alpha cells towards potential beta cells. We utilised an in vitro model of pancreatic alpha cells, the murine αTC1-9 cell line. We initially chara…