Search results for "Prom"

Participatory tools and methods for community action. SESPAS Report 2018

2018

Resumen: La participación es un proceso que requiere la implicación de responsables de la política, del personal directivo y técnico de las instituciones, y de la ciudadanía, así como de la acción intersectorial e interdisciplinaria. Para generar cambios transformadores es necesaria una infraestructura que favorezca la participación y la acción planificada, reconociendo a todos los actores del proceso. Se precisan tiempo y compromiso para su continuidad a través de la producción conjunta de acciones, y de ahí la importancia de consolidar proyectos participativos que continúen más allá de los cambios políticos para que los servicios públicos sean sostenibles. La formación, el uso adecuado de…

Measuring the impact of regional export promotion: The Spanish case

2008

This article estimates the effect of Spanish regional trade agencies abroad on exports using the gravity model. The results indicate that regional agencies increase trade. The estimated impact seems to be larger than that of Spanish embassies and consulates. Moreover, a dis- aggregated analysis shows that this effect is not evenly distributed across Spanish regions. JEL classification: F14, R12

m6A RNA methylation regulates promoter proximal pausing of RNA Polymerase II

2021

AbstractRNA Polymerase II (RNAP II) pausing is essential to precisely control gene expression and is critical for development of metazoans. Here, we show that the m6A RNA modification regulates promoter-proximal RNAP II pausing. The m6A methyltransferase complex (MTC), with the nuclear reader Ythdc1, are recruited to gene promoters. Depleting the m6A MTC leads to a decrease in RNAP II pause release and in Ser2P occupancy on the gene body, and affects nascent RNA transcription. Tethering Mettl3 to a heterologous gene promoter is sufficient to increase RNAP II pause release, an effect that relies on its m6A catalytic domain. Collectively, our data reveal an important link between RNAP II paus…

Respiration and low cAMP-dependent protein kinase activity are required for high-level expression of the peroxisomal thiolase gene in Saccharomyces c…

1996

Transcription of genes for peroxisomal proteins is repressed by glucose and induced by oleate. At least for the peroxisomal thiolase gene (POT1) there is a third regulatory mechanism, mediated by the transcription factor Adr1p, which is responsible for the high-level expression of the gene in stationary phase. Here we show that a region in the POT1 promoter that extends from positions -238 to -152 mediates this mechanism, and we suggest that Adr1p acts indirectly on POT1. We have also analyzed the role of the cAMP-dependent protein kinase (PKA) in the transcriptional regulation of POT1. PKA exerts a negative control: the high, unregulated PKA activity in a bcy1 mutant maintains POT1 transcr…

Materialistic values impact on pro-environmental behavior: The case of transition country as Lithuania

2020

Abstract Materialism is becoming a global phenomenon and is increasing exponentially. Lithuania, which has survived a long period of occupation, experienced privatization and goods’ famine is attributed to the transition countries. In terms of materialistic values, Lithuania is not an exception, as material wealth and image are also very important. Thus, the main question of this paper was whether materialistic values could be reconciled with the pro-environmental behavior, which can be considered as one of the main aspects seeking sustainability. According to the Lithuanian representative survey and by applying the approach of structural equation modeling, the results showed that materiali…

Brewer's spent grains as biofuels in combustion-based energy recovery processes: Evaluation of thermo-oxidative decomposition

2022

[EN] The high global generation of wastes and side streams from agri-food production has led to environmental impact and causes nature degradation due to their uncontrolled management. These wastes are profitable materials with significant economic value that could otherwise be exploited as new sources in the feed industry or the production of bioenergy. Among them, brewer¿s spent grain (BSG) is a solid by-product generated in the beerbrewing process that consists of the barley grain husks together with parts of the pericarp and seed coat layer. Although it is rich in fibres and proteins, its main use is currently limited to animal feed or simply deposition to landfills. This study pursues …

Flanking regions determine the structure of the poly-glutamine homo- repeat in huntingtin through mechanisms common among glutamine-rich human protei…

2020

International audience; The causative agent of Huntington's disease, the poly-Q homo-repeat in the N-terminal region of huntingtin (httex1), is flanked by a 17-residue-long fragment (N17) and a proline-rich region (PRR), which promote and inhibit the aggregation propensity of the protein, respectively, by poorly understood mechanisms. Based on experimental data obtained from site-specifically labeled NMR samples, we derived an ensemble model of httex1 that identified both flanking regions as opposing poly-Q secondary structure promoters. While N17 triggers helicity through a promiscuous hydrogen bond network involving the side chains of the first glutamines in the poly-Q tract, the PRR prom…

A Functional Role of IκB-ε in Endothelial Cell Activation

2000

Abstract The NF-κB inhibitor IκB-ε is a new member of the IκB protein family, but its functional role in regulating NF-κB-mediated induction of adhesion molecule expression is unknown. In vascular endothelial cells, IκB-ε associates predominantly with the NF-κB subunit Rel A and to a lesser extent with c-Rel, whereas IκB-α and IκB-β associate with Rel A only. Following stimulation with TNF-α, pyrrolidine dithiocarbamate (PDTC), N-acetylcysteine, and dexamethasone prevented IκB kinase-induced IκB-α, but not IκB-β or IκB-ε phosphorylation and degradation. Since the activation of NF-κB is required for the induction of adhesion molecule expression, we examined the role of IκB-ε in the transacti…

Evaluation of the use of phase-specific gene promoters for the expression of enological enzymes in an industrial wine yeast strain

1996

Genes as POT1, HSP104 and SSA3, which are late expressed in laboratory culture conditions are expressed only during the first few days in microvinifications in wine yeast cells. This effect is probably due to the different growth conditions and leads to useless levels of enzyme activity for a reporter gene. However the ACT1 promoter, which is constitutively expressed in laboratory conditions, produces sufficient amounts of enzyme activity in late fermentation phases.

2015

Transporters of the ATP-binding cassette (ABC) family such as MDR1 play a pivotal role in persistence of brain homeostasis by contributing to the strict permeability properties of the blood–brain barrier. This barrier on one hand compromises treatment of central nervous system diseases by restricting access of drugs; on the other hand, an impaired or altered function of barrier building cells has been described in neurological disorders. The latter might contribute to increased vulnerability of the brain under pathological conditions or even enforce pathogenesis. Here, we present a novel approach for a systematic examination of drug impact on Mdr1 gene expression by establishing a dual repo…