Search results for "Promoter"

The transcription factor IRF1 dictates the IL-21-dependent anticancer functions of TH9 cells

2014

The TH9 subset of helper T cells was initially shown to contribute to the induction of autoimmune and allergic diseases, but subsequent evidence has suggested that these cells also exert antitumor activities. However, the molecular events that account for their effector properties are elusive. Here we found that the transcription factor IRF1 enhanced the effector function of TH9 cells and dictated their anticancer properties. Under TH9-skewing conditions, interleukin 1β (IL-1β) induced phosphorylation of the transcription factor STAT1 and subsequent expression of IRF1, which bound to the promoters of Il9 and Il21 and enhanced secretion of the cytokines IL-9 and IL-21 from TH9 cells. Further…

PSMA6 gēna promotera poliadenozīna trakta polimorfismu asociācija ar bronhiālo astmu, aptaukošanos un 1. tipa cukura diabētu Latvijas populācijā

2015

Ubikitīna – proteasomu sistēma ir multienzīmatisks proteāžu komplekss, kas regule dažādus šūnu procesus. Šis sistēmas disregulācija var būt iesaistīta tādos cilvēka patoloģiju rašanās mehanismos kā 2. tipa cukura diabēts, juvenīlais idiopātiskais artrīts un Greivsa slimība. Tas var būt atkarīgs no proteasomas gēnu strukturālām pārmaiņām, kuri kodē proteasomu apakšvienības. Šajā pētījumā mēs analizējam asociāciju starp PSMA6 gēna promotera poly(A) (c. –655 An) mikrosatelīta garuma izmaiņām un bronhiālo astmu (BA), 1. tipa cukura diabētu (T1DM) un aptaukošanos (APT) Latvijas populācijā. PSMA6 gēna poly(A) polimorfisms tika analizēts ar gadījuma / kontroles pētījumu un bioinformātiskām program…

PTHrP isoform expression in adipo- and osteo-differentiating human mesenchymal stem cells

2011

Promoção da saúde: O renascimento de uma ideologia

2004

A concepção actual de promoção de saúde, educação para a saúde e prevenção da doença é suportada por ideologias algo adulteradas pela realidade social. Tendo em conta a proliferação epistemológica destes conceitos no final do século XX pretende-se, no presente artigo, elaborar uma reflexão crítica em torno das múltiplas racionalidades estratégicas em que os conceitos se organizam, bem como, abordar os paradoxos que se instalam ao nível dos determinantes biopsicossociais dos comportamentos salutogénicos. Partilhando responsabilidades, os profissionais de saúde, devem, colectiva e proactivamente, desempenhar um papel significativo como promotores de saúde, modelando e reforçando o significado…

Synaptopodin regulates denervation-induced homeostatic synaptic plasticity

2013

Synaptopodin (SP) is a marker and essential component of the spine apparatus (SA), an enigmatic cellular organelle composed of stacked smooth endoplasmic reticulum that has been linked to synaptic plasticity. However, SP/SA-mediated synaptic plasticity remains incompletely understood. To study the role of SP/SA in homeostatic synaptic plasticity we here used denervation-induced synaptic scaling of mouse dentate granule cells as a model system. This form of plasticity is of considerable interest in the context of neurological diseases that are associated with the loss of neurons and subsequent denervation of connected brain regions. In entorhino-hippocampal slice cultures prepared from SP-de…

Nitric oxide synthase: expression and expressional control of the three isoforms.

1995

Three isozymes of nitric oxide synthase (NOS) have been identified. Their cDNA- and protein structures as well as their genomic DNA structures have been described. NOS I (ncNOS, originally discovered in neurons) and NOS III (ecNOS, originally discovered in endothelial cells) are low output, Ca(2+)-activated enzymes whose physiological function is signal transduction. NOS II (iNOS, originally discovered in cytokine-induced macrophages) is a high output enzyme which produces toxic amounts of NO that represent an important component of the antimicrobial, antiparasitic and antineoplastic activity of these cells. Depending on the species, NOS II activity is largely (human) or completely (mouse a…

Structure of the phenylalanine hydroxylase gene in Drosophila melanogaster and evidence of alternative promoter usage.

1996

The complete Drosophila melanogaster phenylalanine hydroxylase gene isolated from a genomic library was sequenced. Gene structure consisted of five exons covering a region of around 3 kb. Position of introns in the C-terminal domain was conserved with mammalian aromatic amino acid hydroxylase genes. Putative promoter sequences in the 5'UTR and intron 1 were identified. A novel transcript was detected differing from that previously reported by the inclusion of a part of the intron 1 sequence. It could be produced using an alternative promoter. The deduced open reading frame would code a protein with a small difference at the N-terminus. Expression of the alternative transcripts was examined …

Computational Chromosome Conformation Capture by Correlation of ChIP-seq at CTCF motifs

2018

Background: Transcription factors (TFs) bind to gene promoters or distal regulatory elements that interact with the promoter via chromatin looping. While the TF binding sites themselves are detected genome-wide by ChIP-seq experiments, it is difficult to associate them regulated genes without information of chromatin looping. Recent experimental techniques such as Hi-C or ChIA-PET measure long-range interactions genome-wide but are experimentally elaborate and have limited resolution. Here, we present Computational Chromosome Conformation Capture by Correlation of ChIP-seq at CTCF motifs (7C). Results: While ChIP-seq was not designed to detect contacts, the formaldehyde treatment in the ChI…

Functioning of DcuC as the C 4 -Dicarboxylate Carrier during Glucose Fermentation by Escherichia coli

1999

ABSTRACT The dcuC gene of Escherichia coli encodes an alternative C 4 -dicarboxylate carrier (DcuC) with low transport activity. The expression of dcuC was investigated. dcuC was expressed only under anaerobic conditions; nitrate and fumarate caused slight repression and stimulation of expression, respectively. Anaerobic induction depended mainly on the transcriptional regulator FNR. Fumarate stimulation was independent of the fumarate response regulator DcuR. The expression of dcuC was not significantly inhibited by glucose, assigning a role to DcuC during glucose fermentation. The inactivation of dcuC increased fumarate-succinate exchange and fumarate uptake by DcuA and DcuB, suggesting a…

The PVT-1 oncogene is a Myc protein target that is overexpressed in transformed cells

2007

The human PVT-1 gene is located on chromosome 8 telomeric to the c-Myc gene and it is frequently involved in the translocations occurring in variant Burkitt's lymphomas and murine plasmacytomas. It has been proposed that PVT-1 regulates c-Myc gene transcription over a long distance. To get new insights into the functional relationships between the two genes, we have investigated PVT-1 and c-Myc expression in normal human tissues and in transformed cells. Our findings indicate that PVT-1 expression is restricted to a relative low number of normal tissues compared to the wide distribution of c-Myc mRNA, whereas the gene is highly expressed in many transformed cell types including neuroblastom…