Search results for "Protein Chemistry"

showing 3 items of 13 documents

Let it flow: Morpholino knockdown in zebrafish embryos reveals a pro-angiogenic effect of the metalloprotease meprin alpha2.

2010

BACKGROUND: Meprin metalloproteases are thought to be involved in basic physiological functions such as cell proliferation and tissue differentiation. However, the specific functions of these enzymes are still ambiguous, although a variety of growth factors and structural proteins have been identified as meprin substrates. The discovery of meprins alpha(1), alpha(2) and beta in teleost fish provided the basis for uncovering their physiological functions by gene silencing in vivo. METHODOLOGY/PRINCIPAL FINDINGS: A Morpholino knockdown in zebrafish embryos targeting meprin alpha(1) and beta mRNA caused defects in general tissue differentiation. But meprin alpha(2) morphants were affected more…

Vascular Endothelial Growth Factor AMorpholinoAngiogenesisMorpholinesCellular differentiationlcsh:MedicineCell Biology/Cell SignalingBiochemistry/Protein ChemistryAnimalsGene silencingCardiovascular Disorders/Vascular Biologylcsh:ScienceZebrafishZebrafishMultidisciplinarybiologyCell growthPhysiology/Cardiovascular Physiology and Circulationlcsh:RMetalloendopeptidasesMorphantCardiovascular Disorders/Cardiovascular Imagingbiology.organism_classificationMolecular biologyCell biologyVascular endothelial growth factor AGene Knockdown TechniquesAngiogenesis Inducing Agentslcsh:QResearch ArticlePLoS ONE
researchProduct

Synthesis of organometallic glycopeptides and electrochemical studies to detect autoantibodies in multiple sclerosis patients' sera

2009

Feliciana Real-Fernandez, Amelie Chamois-Colson, Jerome Bayardon, Francesca Nuti, Elisa Peroni, Maria R. Moncelli, Rita Meunier-Prest, Sylvain Juge and Anna Maria Papini Laboratory of Peptide & Protein Chemistry & Biology, Polo Scientifico, University of Florence, I-50019 Sesto Fiorentino (FI), Italy; Laboratoire de Synthese et d’Electrosynthese Organometalliques (LSEO), Universite de Bourgogne, 21068, Dijon, France

autoantibodiesPeptideProtein chemistrymultiple sclerosis03 medical and health sciences0302 clinical medicinebloodmedicineProtein antigen030304 developmental biologychemistry.chemical_classification0303 health sciencesMultiple sclerosisAutoantibodyglycopeptides[ SDV.SPEE ] Life Sciences [q-bio]/Santé publique et épidémiologiemedicine.diseaseMolecular biologyGlycopeptide3. Good healthchemistryelectrochemistryImmunology[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologieenzyme-linked immunosorbent assay030217 neurology & neurosurgery
researchProduct

Size Exclusion Chromatography of Proteins on Improved Bonded Silica Columns

1985

Publisher Summary This chapter discusses the size exclusion chromatography (SEC) of proteins on improved bonded silica columns. Over the past few years, the use of high performance liquid chromatography (HPLC) has been extended to protein chemistry as a powerful separation and isolation technique. A spectrum of variants providing high resolution potential—ion exchange, reversed phase, hydrophobic interaction, affinity, and size exclusion—is available. Of these, SEC is the simplest with respect to method development and equipment design: proteins are separated according to their size in a short and predictable time span under isocratic conditions. The SEC columns are packed with micro-partic…

chemistry.chemical_classificationHydrophobic effectChromatographychemistryResolution (mass spectrometry)Phase (matter)Size-exclusion chromatographyHigh resolutionPolymerProtein chemistryHigh-performance liquid chromatography
researchProduct