Search results for "Protéine"

Activités cytotoxiques et pro-oxydantes d'acides gras à très longue chaîne sur des oligodendrocytes murins sauvages et déficients en Abcd1 et Acox1 :…

2010

X-ALD and P-NALD are two rare, peroxisomal metabolic and neurodegenerative diseases. ABCD1 and ACOX1 are known to be responsible for X-ALD and P-NALD, respectively. The actively demyelinating lesions in CNS, exhibited signs of oxidative stress and cell death. The accumulation of VLCFA in plasma and tissue is the biochemical common hallmark to both diseases. First, we characterized a murine oligodendrocytes cell line 158N to use it as a model. This 158N cell line which has characteristics of mature oligodendrocytes (expression of myelin proteins MOG, MBP, PLP), has also functional peroxisomes with Abcd1 and Acox1 proteins. Then, we studied the cytotoxic and pro-oxidative effects of VLCFA (C2…

PULSES ! Interroger les représentations sociales afin d'identifier des leviers en faveur d'un rééquilibrage entre protéines animales et végétales

2018

National audience

Comprehension and food analysis of a fermented mixture of animal proteins and vegetable proteins : influence on the physico-chemistry and the accepta…

2017

Many problems have been identified following the attempts to incorporate vegetal proteins in our food. In particular, off-flavor, taste or texture have been highlighted as real barriers to the acceptability of plant products by consumers. The consumer himself is also an important determinant regarding the term "acceptability." So, in order to develop a new fermented product based on pea, two issues were studied: the product and the consumer. To understand the physico-chemical properties and the acceptability of pea protein-based fermented products, several factors have been investigated in successive stages such as culture, positive health and environmental claims, lactic acid bacteria stra…

Déterminisme génétique de la plasticité de la composition protéique des graines de légumineuses vis-à-vis de l'environnement : rôle du métabolisme du…

2021

The renewed interest in plant proteins has stimulated research aimed at developing markers to aid in the selection of legume varieties better adapted to nutritional needs. Among the traits to be improved and stabilized is the amino acid balance of seeds, the essential amino acids methionine and tryptophan being particularly under-represented in legume seeds. This thesis focuses on seed protein composition, which is a major determinant of the seed amino acid balance. The objective was to explore the genetic and environmental variability in this trait and to identify genes potentially involved in its plasticity when subjected to environmental stresses. In the first part of the thesis, the pro…

Production of sweet-tasting proteins in yeast: achievements and perspectives

2015

Optimiser et stabiliser la composition protéique des graines de légumineuses

2018

International audience

Relationships between sodium mobility, in-mouth salt and aroma release, and flavour perception : application to model cheeses

2012

The aim of this work is to understand the effects of changes in the composition of model cheeses on mobility, release and perception of flavor molecules (salt, aroma compounds). Six flavoured model cheeses were formulated (3 lipid/protein (L/P) ratios and 2 salt contents). The microstructure and the rheological properties of the model cheeses were characterized respectively by confocal microscopy and by uniaxial compression test. The mobility of sodium ions was analyzed by 23Na NMR. The kinetics of sodium release was followed in water and then in saliva during consumption of the model cheeses. The retronasal release of aroma compounds was followed by nose-space APCI-MS, simultaneously with …

Regulation of the activity of the Cholesteryl Ester Tranfer Protein (CETP) : electrostatics interactions with carboxylic derivatives (Non Esterified …

1997

In human plasma, the cholesteryl ester transfer protein (CETP) plays a key role in lipoprotein metabolism by promoting the net mass transfer of cholesteryl esters from HDL towards apoB-containing lipoproteins, and the transfer of triglycerides from VLDL towards HDL and LDL. Thus CETP arises as a potentially important factor in plasma lipoproteins metabolism, a statement which raised a considerable interest in identifying the factors which may regulate CETP activity.In our in vitro studies, we demonstrated that amphipathic molecules (non esterified fatty acids and retinoic acids) constituted of one carboxylic head group and one hydrophobic carbon chain are able to increase the cholesteryl es…

The skeleton of the staghorn coral Acropora millepora: molecular and structural characterization.

2014

15 pages; International audience; The scleractinian coral Acropora millepora is one of the most studied species from the Great Barrier Reef. This species has been used to understand evolutionary, immune and developmental processes in cnidarians. It has also been subject of several ecological studies in order to elucidate reef responses to environmental changes such as temperature rise and ocean acidification (OA). In these contexts, several nucleic acid resources were made available. When combined to a recent proteomic analysis of the coral skeletal organic matrix (SOM), they enabled the identification of several skeletal matrix proteins, making A. millepora into an emerging model for biomi…

The Recent Development of a Sweet-Tasting Brazzein and its Potential Industrial Applications

2016

International audience; Brazzein is a small heat- and pH-stable sweet-tasting protein isolated from the West African plant, Pentadiplandra brazzeana. Brazzein combines a highly sweet potency, a long history of human consumption, and a remarkable stability, giving it great potential as a natural sweetener. Due to the difficulties of obtaining brazzein from its natural source, several efforts have been made to express brazzein using various heterologous expression systems. This chapter describes the biochemical, structural, sensory, and physiological properties of brazzein. We will summarize the current knowledge of the structure-activity relationship of brazzein. The biotechnological product…