Search results for "Pseudomonas Aeruginosa"
showing 10 items of 158 documents
Cladosins L-O, new hybrid polyketides from the endophytic fungus Cladosporium sphaerospermum WBS017
2019
Abstract The endophytic fungus Cladosporium sphaerospermum WBS017 was obtained from healthy bulbs of Fritillaria unibracteata var. wabuensis. Fermentation of C. sphaerospermum on solid rice medium yielded three new hybrid polyketides, cladosins L−N (1–3), and a known derivative cladodionen (4). Further cultivation of this fungus on white bean medium afforded an additional new hybrid polyketide, cladosin O (5) along with three known analogues (6–8). The structures of the new compounds were elucidated using a combination of NMR and HRESIMS data. The absolute configurations of compounds 2 and 3 were determined by Mosher’s method and TDDFT-ECD calculations. All isolated compounds were evaluated…
High susceptibility of MDR and XDR Gram-negative pathogens to biphenyl-diacetylene-based difluoromethyl-allo-threonyl-hydroxamate LpxC inhibitors
2016
International audience; Inhibitors of uridine diphosphate-3-O-(R-3-hydroxymyristoyl)-N-acetylglucosamine deacetylase (LpxC, which catalyses the first, irreversible step in lipid A biosynthesis) are a promising new class of antibiotics against Gram-negative bacteria. The objectives of the present study were to: (i) compare the antibiotic activities of three LpxC inhibitors (LPC-058, LPC-011 and LPC-087) and the reference inhibitor CHIR-090 against Gram-negative bacilli (including MDR and XDR isolates); and (ii) investigate the effect of combining these inhibitors with conventional antibiotics.MethodsMICs were determined for 369 clinical isolates (234 Enterobacteriaceae and 135 non-fermentati…
Pseudomonas aeruginosa un Acinetobacter baumanii jutība pret antibakteriālajiem līdzekļiem
2018
Pseudomonas aeruginosa un Acinetobacter baumannii ir galvenie ar veselības aprūpes saistīto infekciju (VASI) izraisītāji. Šo patogēnu rezistence pret antibakteriālajiem līdzekļiem pēdējo gadu laikā ir strauji palielinājusies, un ir viena no aktuālākajām problēmām mūsdienās. Darba mērķis: izpētīt gramnegatīvo baktēriju P. aeruginosa un A. baumannii jutību pret antibakteriālajiem līdzekļiem, salīdzināt antibakteriālās rezistences izmaiņas divu gadu laikā. Uzdevumi: identificēt no elpceļiem iegūtos izolātus ar BBL™ Crystal™ sistēmu, veikt antibakteriālo līdzekļu jutības testus, izmantojot Bauer – Kirby disku difūzijas testu un E – testu, fenotipēšanu veikt pēc antibiogrammām. Rezultātus apko…
Acinetobacter baumannii un Pseudomonas aeruginosa antibakteriālās rezistences noteikšana, lietojot fenotipiskās un molekulāri bioloģiskās metodes
2020
Acinetobacter baumannii un Pseudomonas aeruginosa ir gan Latvijā, gan visā Eiropā bieži sastopami intrahospitāli patogēni, pret kuriem antibiotiku rezistence pieaug ik gadu. Visbiežāk tie skar imūnsupresētus cilvēkus, apdraudot veselību un dzīvību. Darba mērķis: Noteikt A.baumannii un P. aeruginosa jutību pret antibakteriālajiem līdzekļiem 2019. gadā un salīdzināt rezistences izmaiņas ar 2017. gadu, kā arī ar molekulāri bioloģisko metodi identificēt baktēriju sugas. Uzdevumi: 1.No elpceļu paraugiem ar BBL CrystalTM identifikācijas sistēmām identificēt izolātus. 2. Noteikt antimikrobiālo jutību, izmantojot Bauer-KirbyTM disku difūzijas testu un MIC, lietojot ETM-testu. 3. Tipēt izdalīto bakt…
Risk factors for cystic fibrosis arthropathy: Data from the German cystic fibrosis registry
2021
Epidemiology and potential risk factors for cystic fibrosis arthropathy (CFA) were studied in a relevant cystic fibrosis (CF) patient cohort.Cohort study of patients included in the German CF registry in 2016-2017. Descriptive analysis, exploratory tests and multivariable logistic regression were used to assess prevalence of CFA and associated potential risk factors for adult patients with/without chronic Pseudomonas aeruginosa infection.6069 CF patients aged from 0 to 78 years were analysed. CFA was observed in 4.9% of the patients. Prevalence was significantly higher in adult patients (8.4%) compared to patients18 years (0.7%; p0.0001). Logistic regression analyses in adult patients (n=33…
Ceftolozane Pharmacokinetics in a Septic Critically Ill Patient under Different Extracorporeal Replacement Therapies
2019
Ceftolozane-tazobactam (C/T), a novel fifth-generation cephalosporin/β-lactamase inhibitor combination active against multidrug-resistant (MDR) Pseudomonas aeruginosa, is currently approved by the U.S. Food and Drug Administration (FDA) to treat complicated intra-abdominal and urinary tract
Can technical, functional and structural characteristics of dental units predict Legionella pneumophila and Pseudomonas aeruginosa contamination?
2011
Legionella pneumophila and Pseudomonas aeruginosa are common colonizers of water environments, particularly dental unit waterlines. The aim of this study was to assess whether the technical, functional and structural characteristics of dental units can influence the presence and the levels of opportunistic pathogens. Overall, 42 water samples were collected from dental units in a teaching hospital in Palermo, Italy, including 21 samples from the 21 taps supplied by the municipal water distribution system and 21 samples from oral rinsing cups at 21 dental units. L. pneumophila was present in 16 out of 21 water samples (76.2%) from dental units, and the median concentration was higher in samp…
Antimicrobial activity of biogenically produced spherical Se-nanomaterials embedded in organic material against Pseudomonas aeruginosa and Staphyloco…
2017
In an effort to prevent the formation of pathogenic biofilms on hydroxyapatite (HA)-based clinical devices and surfaces, we present a study evaluating the antimicrobial efficacy of Spherical biogenic Se-Nanostructures Embedded in Organic material (Bio Se-NEMO-S) produced by Bacillus mycoides SelTE01 in comparison with two different chemical selenium nanoparticle (SeNP) classes. These nanomaterials have been studied as potential antimicrobials for eradication of established HA-grown biofilms, for preventing biofilm formation on HA-coated surfaces and for inhibition of planktonic cell growth of Pseudomonas aeruginosa NCTC 12934 and Staphylococcus aureus ATCC 25923. Bio Se-NEMO resulted more e…
Anti-Pseudomonas aeruginosa activity of hemlock (Conium maculatum, Apiaceae) essential oil
2018
Conium maculatum is a nitrophilous weed belonging to the Apiaceae family and occurring in hedgerows, pastures, waste ground, along rivers and roadsides. Little is known on the chemistry and bioactivity of other secondary metabolites occurring in the plant. In the present work, we have analysed the chemical composition and antimicrobial activity of the essential oils hydrodistilled from leaves and inflorescenes of C. maculatum growing in Sicily, Italy. The composition of essential oils was achieved by gas chromatography-mass spectrometry (GC-MS) analysis, whereas the inhibitory effects on the growth of two Gram negative strains, namely Escherichia coli and Pseudomonas aeruginosa were assesse…
Comparison of pulsed-field gel electrophoresis and whole-genome-sequencing-based typing confirms the accuracy of pulsed-field gel electrophoresis for…
2020
Summary Aim To determine whether pulsed-field gel electrophoresis (PFGE) accurately recognizes isolates belonging to clusters defined by techniques based on whole-genome sequencing (WGS) using Pseudomonas aeruginosa as a model. Methods We selected 65 isolates of ST395 P. aeruginosa isolated in seven European hospitals between 1998 and 2012. Isolates were typed by PFGE and sequenced by WGS. A core genome multi-locus sequence typing (cgMLST) analysis based on 3831 genes was performed with a homemade pipeline. Findings PFGE identified eight pulsotypes and cgMLST differentiated nine clusters and nine singletons. Five cgMLST clusters and pulsotypes (31/65 isolates) coincided perfectly. Isolates …