Search results for "QToF"

showing 10 items of 14 documents

Combined use of liquid chromatography triple quadrupole mass spectrometry and liquid chromatography quadrupole time-of-flight mass spectrometry in sy…

2012

As a suitable way for routine screening of pesticides and control of other organic contaminants in water, the combination of liquid chromatography triple quadrupole tandem mass spectrometry (LC-QqQ-MS/MS) and liquid chromatography-hybrid quadrupole time-of-flight mass spectrometry (LC-QTOF-MS) has been applied to the analysis of 63 surface and waste water samples after conventional solid-phase extraction (SPE). The extracts were screened for 43 pesticides or degradation products by LC-QqQ-MS/MS achieving limits of detection (LOD) ranged from 0.04 to 2 ng L(-1). Of the 43 selected pesticides, 33 were detected in water samples. The ESI-QTOF MS instrument was run using two simultaneous acquisi…

Tandem mass spectrometryMass spectrometryBiochemistryAnalytical ChemistryLC–MS/MSLimit of DetectionTandem Mass SpectrometryEnvironmental ChemistrySample preparationSolid phase extractionPesticidesChromatography High Pressure LiquidSpectroscopyDetection limitChromatographyChemistrySolid Phase ExtractionExtraction (chemistry)WaterSurface waterTriple quadrupole mass spectrometerLC–QTOF MSMass spectrumOrganic pollutantsWaste waterWater Pollutants ChemicalAnalytica Chimica Acta
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Contaminants of emerging concern in the environment. Turia River as study case

2018

Tesis llevada a cabo para conseguir el grado de Doctor por la Universidad de Valencia.--2018-04-05

drogas de abusoQuEChERScontaminantes emergentesmétodo analíticotriple quadrupolo:QUÍMICA::Química analítica::Análisis cromatográfico [UNESCO]Tampón de McIlvaineMS/MSProductos de cuidado personalUNESCO::QUÍMICA::Química analítica::Análisis cromatográficoUNESCO::CIENCIAS DE LA TIERRA Y DEL ESPACIO::Hidrología ::Aguas superficialesHPLCQToFfármacos:CIENCIAS DE LA TIERRA Y DEL ESPACIO::Hidrología ::Aguas superficiales [UNESCO]Extracción en fase sólida
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Chemical composition, cytotoxic effects, antimicrobial and antibiofilm activity of Artemisia arborescens (Vaill.) L. growing wild in the province of …

2022

Artemisia arborescens (Vaill.) L. is a perennial shrubby plant growing along the coastal strips of the Mediterranean region. It is used in traditional medicine. Its essential oil and solvent extracts exhibit a very interesting chemotherapeutic potential, which makes this plant useful in maintaining human health. The goal of this study was to determine the phytochemical composition of the petroleum ether and methanol extracts, as well as to evaluate anticancer activities and antimicrobial and biofilm formation reduction. Thirty-nine phytochemical compounds in negative ion mode, and 25 in positive ion mode were identified by HPLC-ESI-QTOF-MS. All four extracts reduced the viability of human M…

Settore BIO/10 - BiochimicaSettore BIO/14 - FarmacologiaPlant ScienceSettore BIO/19 - Microbiologia GeneraleSettore CHIM/08 - Chimica FarmaceuticaArtemisia arborescens (Vaill.) L. HPLC-ESI-QTOF-MS antitumor effects antimicrobial activity antibiofilm activityEcology Evolution Behavior and SystematicsPlant Biosystems - An International Journal Dealing with all Aspects of Plant Biology
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Phytochemical-rich extracts of Helianthemum lippii possess antimicrobial, anticancer, and anti-biofilm activities

2022

Helianthemum lippii is a perennial shrubby plant growing in the sandy environments of Italy, Mediterranean countries of North Africa and Middle East. H. lippii is used in traditional medicine but there are very few reports referring to the phytochemical characterization, the ethnopharmacology, and the biological activity of H. lippii. The goal of this study was todetermine the phytochemical composition of different H. lippii extracts, cold (CME) and hot (HME) methanol, cold (CPEE) and hot (HPEE) petroleum ether, as well as to evaluate their anticancer and antimicrobial activities and biofilm formation reduction. Fifty-fours phytocompounds have been determined by HPLC-UV-ESI-QTOF-MS analysis…

HPLC-UV-ESI-QTOF-MS analysielianthemum lippiCytotoxic effectAntibiofilm activitySettore BIO/10 - BiochimicaSettore BIO/02 - Botanica SistematicaSettore BIO/14 - FarmacologiaPlant ScienceAntimicrobial activitySettore BIO/19 - Microbiologia GeneraleSettore CHIM/08 - Chimica FarmaceuticaEcology Evolution Behavior and SystematicsPlant Biosystems - An International Journal Dealing with all Aspects of Plant Biology
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Untargeted metabolomics to explore the oxidation processes during shelf life of pork patties treated with guarana seed extracts

2019

The changes of metabolites in pork patties with different antioxidants added (control without antioxidants, 200 mg kg(-1) butylated hydroxytoluene (BHT) and 250 mg kg(-1) guarana seed extracts (GSEs)) under modified atmosphere (80% O-2 and 20% CO2) for 18 days of refrigerated storage were evaluated. Untargeted metabolomic approach based on UHPLC-ESI-QTOF-MS analysis was applied. GSE phytochemical profile revealed a wide variety of compounds (caffeine, glycerol 1-propanoate, amino acids, alkaloids and glycerophospholipids), together with antioxidants (tyrosols, procyanidins and flavonoids). Important differences in BHT and GSE patties metabolomic profiles were found during storage. Most of t…

2. Zero hungerfood metabolomics010401 analytical chemistrySpermine04 agricultural and veterinary sciencesProtein degradationGlycerophospholipidsShelf life040401 food science01 natural sciencesAntioxidantsIndustrial and Manufacturing Engineering0104 chemical scienceschemistry.chemical_compoundPaulinia cupana0404 agricultural biotechnologylipid oxidationLipid oxidationchemistryPhytochemicalUHPLC-QTOFModified atmosphereButylated hydroxytolueneFood scienceFood ScienceInternational Journal of Food Science & Technology
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Molecular Characterization of White Wines Antioxidant Metabolome by Ultra High Performance Liquid Chromatography High-Resolution Mass Spectrometry

2020

International audience; The knowledge about the molecular fraction contributing to white wines oxidative stability is still poorly understood. However, the role of S- and N-containing compounds, like glutathione and other peptides, as a source of reductant in many oxidation reactions, and acting against heavy metals toxicity, or lipid and polyphenol oxidation as ROS-scavenger is today very well established. In that respect, the aim of the present study is to introduce an original analytical tool for the direct determination of the available nucleophilic compounds in white wine under acidic pH conditions. One step derivatization of nucleophiles has been realized directly in wines using 4-met…

AntioxidantUHPLC-QqTOF-MSPhysiologymedicine.medical_treatmentClinical BiochemistrynucleophilesMass spectrometry01 natural sciencesBiochemistryArticlechemistry.chemical_compound0404 agricultural biotechnologymedicineMetabolomeDerivatizationuntargeted analysisMolecular BiologyMETLINthiolsWineChromatographyChardonnay wine oxidationChemistry010401 analytical chemistrylcsh:RM1-950food and beverages04 agricultural and veterinary sciencesCell Biology040401 food science0104 chemical sciencesQuinonelcsh:Therapeutics. PharmacologyPolyphenolpeptides[SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
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Determination of multiple mycotoxins in feedstuffs by combined use of UPLC–MS/MS and UPLC–QTOF–MS

2018

In this report, a UPLC-ESI-MS/MS method for the simultaneous determination of aflatoxins, ochratoxin A, zearalenone, deoxynivalenol, fumonisins, T-2 and HT-2 toxins, fusarenone X, diacetoxyscirpenol, and 3- and 15-acetyldeoxynivalenol in feedstuffs was developed. A quadrupole-time-of-flight mass spectrometer detector (QTOF-MS) operating in full scan mode was combined with the UPLC-ESI-MS/MS system to confirm the identity of detected mycotoxins and to identify other possible microbial metabolites occurring in samples. Sixty-two feed samples from the Spanish market were analyzed. Extraction of metabolites was carried out with acetonitrile-water-formic acid (80:19:1, v/v/v). Method detection a…

Ochratoxin AAflatoxinMass spectrometryFumonisins01 natural sciencesMass SpectrometryDiacetoxyscirpenolAnalytical Chemistrychemistry.chemical_compound0404 agricultural biotechnologyAflatoxinsMycotoxinZearalenoneChromatography High Pressure LiquidChromatography010401 analytical chemistry04 agricultural and veterinary sciencesGeneral MedicineMycotoxinsAnimal FeedOchratoxins040401 food science0104 chemical sciencesT-2 ToxinchemistryUplc qtof msZearalenoneUplc ms msTrichothecenesFood ScienceFood Chemistry
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Metabolic signatures across the full spectrum of non-alcoholic fatty liver disease.

2022

Funder: European Commission

ALTtype 2 diabetes mellitusROC receiving operator characteristicaspartate aminotransferaseHSDLDL low-density lipoproteinUHPLC ultrahigh-performance liquid chromatographyROCHCCNon-alcoholic steatohepatitisGCPCANASHGastroenterology2-HB 2-hydroxybutanoic acid; 3-HB 3-hydroxybutanoic acid; ALT alanine aminotransferase; AST aspartate aminotransferase; CE cholesterol ester; Cer ceramide; FFA free fatty acid; FLIP Fatty Liver Inhibition of Progression; Fibrosis; GC gas chromatography; HCC hepatocellular carcinoma; HSD honest significant difference; LC lipid cluster; LDL low-density lipoprotein; LM lipid and metabolite; LMC lipid metabolite and clinical variable; LPC lysophosphatidylcholine; Lipidomics; Mass spectrometry; Metabolomics; NAFL non-alcoholic fatty liver; NAFLD non-alcoholic fatty liver disease; NAS NASH activity score; NASH non-alcoholic steatohepatitis; NIDDK NASH-CRN National Institute of Digestive Diseases and Kidney NASH Clinical Research Network; NRR non-rejection rate; Non-alcoholic steatohepatitis; PC(O) ether PC; PC phosphatidylcholine; PCA principal component analysis; PE phosphatidylethanolamine; QTOFMS quadrupole-time-of-flight mass spectrometry; ROC receiving operator characteristic; SAF steatosis activity and fibrosis; SM sphingomyelin; T2DM type 2 diabetes mellitus; TG triacylglycerol; UHPLC ultrahigh-performance liquid chromatographySAFSAF steatosis activity and fibrosisLM lipid and metabolitehonest significant differenceHSD honest significant differenceTG triacylglycerolnon-rejection ratecholesterol esterPCPEGC gas chromatographyfree fatty acidFLIPNASH non-alcoholic steatohepatitisNIDDK NASH-CRN National Institute of Digestive Diseases and Kidney NASH Clinical Research NetworkBIOMARKERST2DMPE phosphatidylethanolamineLDLlipidNAFLDFFA free fatty acid2-HBMetabolomicsNAFL non-alcoholic fatty liverLMCphosphatidylcholineScience & TechnologySM sphingomyelinGastroenterology & HepatologyMass spectrometryactivitynutritional and metabolic diseasesT2DM type 2 diabetes mellitusACIDSreceiving operator characteristicdigestive system diseasesquadrupole-time-of-flight mass spectrometryLC lipid clusterlow-density lipoproteinNAS2-HB 2-hydroxybutanoic acidNAS NASH activity scoreQTOFMSether PCNRRSCORING SYSTEMprincipal component analysisgas chromatographyLC2-hydroxybutanoic acidPROGRESSIONAST aspartate aminotransferaseLMPC phosphatidylcholinePC(O)MARKERSUHPLCsteatosisTOOLImmunology and AllergyINSULIN-RESISTANCECerSMFatty Liver Inhibition of Progressionhepatocellular carcinoma2-HB 2-hydroxybutanoic acid NIDDK NASH-CRN National Institute of Digestive Diseases and Kidney NASH Clinical Research Network NRR non-rejection rate Non-alcoholic steatohepatitis PC(O) ether PC PC phosphatidylcholine PCA principal component analysis PE phosphatidylethanolamine QTOFMS quadrupole-time-of-flight mass spectrometry ROC receiving operator characteristic SAF steatosis activity and fibrosis SM T2DM type 2 diabetes mellitus TG triacylglycerol UHPLC ultrahigh-performance liquid chromatographyultrahigh-performance liquid chromatographyCELPC3-HBNAFLnon-alcoholic fatty liverTGtriacylglycerolNRR non-rejection rateLife Sciences & BiomedicineNAFLD non-alcoholic fatty liver diseaseFLIP Fatty Liver Inhibition of Progressionalanine aminotransferasemetaboliteCer ceramideCE cholesterol estersphingomyelinlysophosphatidylcholineand fibrosisALT alanine aminotransferaseInternal MedicineceramideNational Institute of Digestive Diseases and Kidney NASH Clinical Research NetworkAST3-HB 3-hydroxybutanoic acidQTOFMS quadrupole-time-of-flight mass spectrometryPCA principal component analysisLPC lysophosphatidylcholineHepatologynon-alcoholic fatty liver diseaseand clinical variablePC(O) ether PC3-hydroxybutanoic acidFibrosisNASH activity scoreNIDDK NASH-CRNlipid clusterlipid and metabolitephosphatidylethanolamineLipidomicsLMC lipid metabolite and clinical variableFFAHCC hepatocellular carcinomaJHEP reports : innovation in hepatology
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Assessment of Human Exposure to Deoxynivalenol, Ochratoxin A, Zearalenone and Their Metabolites Biomarker in Urine Samples Using LC-ESI-qTOF.

2021

Human are exposed to a wide range of mycotoxins through dietary food intake, including processed food. Even most of the mycotoxin exposure assessment studies are based on analysis of foodstuffs, and evaluation of dietary intake through food consumption patterns and human biomonitoring methods are rising as a reliable alternative to approach the individual exposures, overcoming the limitations of the indirect dietary assessment. In this study, human urine samples were analyzed, seeking the presence of deoxynivalenol (DON), ochratoxin A (OTA), zearalenone (ZEA), and their metabolites. For this purpose, 40 urine samples from female and male adult residents in the city of Valencia (Spain) were …

Ochratoxin AAdultMaleSpectrometry Mass Electrospray IonizationAdolescentHealth Toxicology and MutagenesisUrineLC-ESI-qTOFToxicology01 natural sciencesArticleExcretionDietary Exposurechemistry.chemical_compoundYoung Adult0404 agricultural biotechnologymycotoxinsBiomonitoringHumansFood scienceMycotoxinZearalenonemetabolitesAgedChemistry010401 analytical chemistryRfood and beveragesbiomarkersrisk assessment04 agricultural and veterinary sciencesMiddle Aged040401 food scienceOchratoxinsurine0104 chemical sciencesHuman exposureBiomarker (medicine)MedicineZearalenoneFemaleTrichothecenesBiological MonitoringChromatography LiquidToxins
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Individual and Combined Effect of Zearalenone Derivates and Beauvericin Mycotoxins on SH-SY5Y Cells

2020

Beauvericin (BEA) and zearalenone derivatives, &alpha

FusariumMTTElectrosprayTime FactorsHealth Toxicology and MutagenesisNeurotoxinslcsh:MedicineToxicologyMass spectrometryArticleSH-SY5Y cells03 medical and health scienceschemistry.chemical_compoundInhibitory Concentration 500404 agricultural biotechnologyCell Line TumorDepsipeptidesHumansMTT assayMycotoxinZearalenone030304 developmental biologyNeurons0303 health scienceszearalenone derivatesChromatographybiologyCell DeathDose-Response Relationship Druglcsh:RbeauvericinDrug Synergism04 agricultural and veterinary sciencesbiology.organism_classification040401 food scienceBeauvericinqTOF–MS/MSchemistryZeranolAntagonismToxins
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