Search results for "R2"
showing 10 items of 285 documents
Recomendación para la determinación de HER2 en cáncer de mama. Consenso nacional de la Sociedad Española de Anatomía Patológica (SEAP) y de la Socied…
2009
La identificación de los carcinomas de mama con amplificación/sobreexpresión de HER2 es crítica en la práctica clínica diaria ya que estas neoplasias requieren un tratamiento específico que incluye el uso de terapias dirigidas. Tanto las técnicas de hibridación in situ como las técnicas inmunohistoquímicas son métodos apropiados para la identificación de cánceres de mama HER2 positivos. Sin embargo, numerosos estudios, incluidos los desarrollados por la Asociación para la Garantía de Calidad en Patología de la SEAP (AGCP) y la experiencia de centros de referencia nacionales en la determinación de HER2 han puesto de manifiesto importantes problemas de reproducibilidad entre laboratorios. Por…
Vizuāli semantiski vaicājumi pār relāciju datubāzēm
2020
Korektu vaicājumu izveide ir svarīgākais posms datu izguves procesā. Konstruējot vaicājumu vizuāli, tiek sniegta iespēja iegūt korektus datus ne tikai jomas speciālistiem, bet arī citu profesiju pārstāvjiem bez specifiskām tekstuālās vaicājuma valodas sintakses zināšanām. Dažādas vaicājumu bāzētas valodas kā SPARQL tiek izmantotas, lai iegūtu datus no semantiskā tīmekļa, bet, izmantojot minēto vaicājumu valodu, nav iespējams tieši izgūt datus no relāciju datubāzēm. Ontoloģijā bāzēta datu piekļuve ir kļuvusi par populāru pieeju lielo datu laikmetā. Darbā tiek apskatīta iespēja OBDA pieejas ietvaros veikt vizuāli semantisku vaicājumu izpildi, pār relāciju datubāzēm izmantojot R2RML. Izmantojo…
An experimental study of a refrigerating plant when replacing R22 with HFCs refrigerants
2011
This paper presents the results of an experimental analysis comparing the performance of a vapour compression refrigerating unit operating with R22, and its performance in comparison to some HFCs fluids, substituting the former according to reg. n. 2037/2000. In particular, the plant working efficiency was first tested with R22 and then with three HFC fluids: R417A, R407C and R404A. The investigation verified that the performance with HFCs refrigerants did not result as efficient as when using R22. An environmental analysis also was performed.
Use of HFC fluids as suitable replacements in low-temperature refrigeration plants
2014
An experimental investigation of the performance of a low-temperature refrigerating unit working with R22 and a comparison of its performance when operating with replacement HFC fluids in accordance with the European Regulation CE-1005/2009 are presented in this paper. Plant working efficiency was tested with R22, as baseline, and then compared with four different HFC fluids: R413A, R417A, R422A and R422D. The refrigerating unit was a vapour-compression plant equipped with a reciprocating double-cylinder compressor able to keep the cold room at -20ºC. Lower values of the temperature at the end of compression and polytrophic exponent can be achieved with the HFC tested. Substituting the R22 …
EBV-Induced Gene 3 Transcription Is Induced by TLR Signaling in Primary Dendritic Cells via NF-κB Activation
2005
Abstract The EBV-induced gene 3 (EBI3) is expressed in dendritic cells (DCs) and part of the cytokine IL-27 that controls Th cell development. However, its regulated expression in DCs is poorly understood. In the present study we demonstrate that EBI3 is expressed in splenic CD8−, CD8+, and plasmacytoid DC subsets and is induced upon TLR signaling. Cloning and functional analysis of the EBI3 promoter using in vivo footprinting and mutagenesis showed that stimulation via TLR2, TLR4, and TLR9 transactivated the promoter in primary DCs via NF-κB and Ets binding sites at −90 and −73 bp upstream of the transcriptional start site, respectively. Furthermore, we observed that NF-κB p50/p65 and PU.1…
Targeted Therapy Modulates the Secretome of Cancer-Associated Fibroblasts to Induce Resistance in HER2-Positive Breast Cancer
2021
The combination of trastuzumab plus pertuzumab plus docetaxel as a first-line therapy in patients with HER2-positive metastatic breast cancer has provided significant clinical benefits compared to trastuzumab plus docetaxel alone. However, despite the therapeutic success of existing therapies targeting HER2, tumours invariably relapse. Therefore, there is an urgent need to improve our understanding of the mechanisms governing resistance, so that specific therapeutic strategies can be developed to provide improved efficacy. It is well known that the tumour microenvironment (TME) has a significant impact on cancer behaviour. Cancer-associated fibroblasts (CAFs) are essential components of the…
Second-generation Langerhans cells originating from epidermal precursors are essential for CD8+ T cell priming.
2014
Abstract In vivo studies questioned the ability of Langerhans cells (LCs) to mediate CD8+ T cell priming. To address this issue, we used intradermal immunization with plasmid DNA, a system in which activation of CD8+ T cells depends on delayed kinetics of Ag presentation. We found that dendritic cells (DCs) located in the skin at the time of immunization have limited ability to activate CD8+ T cells. This activity was mediated by a second generation of DCs that differentiated in the skin several days after immunization, as well as by lymph node–resident DCs. Intriguingly, CD8+ T cell responses were not affected following treatment with clodronate liposomes, immunization of CCR2−/− mice, or …
Locally nilpotent derivations of rings graded by an abelian group
2019
International audience
miR-20b modulates VEGF expression by targeting HIF-1 alpha and STAT3 in MCF-7 breast cancer cells.
2010
MicroRNAs (miRNAs) are small non-coding RNAs that regulate the expression of different genes, including genes involved in cancer progression. A functional link between hypoxia, a key feature of the tumor microenvironment, and miRNA expression has been documented. We investigated whether and how miR-20b can regulate the expression of vascular endothelial growth factor (VEGF) in MCF-7 breast cancer cells under normoxic and hypoxia-mimicking conditions (CoCl(2) exposure). Using immunoblotting, ELISA, and quantitative real-time PCR, we demonstrated that miR-20b decreased VEGF protein levels at 4 and 24 h following CoCl(2) treatment, and VEGF mRNA at 4 h of treatment. In addition, miR-20b reduce…
Rtp1p Is a Karyopherin-Like Protein Required for RNA Polymerase II Biogenesis
2013
The assembly and nuclear transport of RNA polymerase II (RNA pol II) are processes that require the participation of many auxiliary factors. In a yeast genetic screen, we identified a previously uncharacterized gene, YMR185w (renamed RTP1), which encodes a protein required for the nuclear import of RNA pol II. Using protein affinity purification coupled to mass spectrometry, we identified interactions between Rtp1p and members of the R2TP complex. Rtp1p also interacts, to a different extent, with several RNA pol II subunits. The pattern of interactions is compatible with a role for Rtp1p as an assembly factor that participates in the formation of the Rpb2/Rpb3 subassembly complex and its bi…