Search results for "RAPA"
showing 10 items of 471 documents
Preparation, characterization and in vitro test of composites poly-lactic acid/hydroxyapatite scaffolds for bone tissue engineering.
2018
Abstract In this work, the possibility to produce composite Poly-L-lactic acid (PLLA)/Hydroxyapatite (HA) porous scaffolds via Thermally Induced Phase Separation (TIPS) for bone tissue engineering applications was investigated. Several PLLA/HA wt/wt ratios (95/5, 90/10, 70/30, 50/50, 34/66) were tested and the as-obtained scaffolds were characterized via Scanning Electron Microscopy, Wide Angle X-Ray Diffraction, Thermogravimetric analysis, Gas Pycnometry, Differential Scanning Calorimetry and mechanical compression test. Morphological analysis revealed an open structure with interconnected pores and HA particles embedded in the polymer matrix. Finally, cell cultures were carried out into t…
In vivo comparison of a granular and putty form of a sintered and a non-sintered silica-enhanced hydroxyapatite bone substitute material
2019
Improved handling characteristics of bone substitute materials may facilitate surgical handling. However, the respective modifications should not alter the biological activity. For comparison of a ...
In human endothelial cells rapamycin causes mTORC2 inhibition and impairs cell viability and function.
2008
Aim Drug-eluting stents are widely used to prevent restenosis but are associated with late endothelial damage. To understand the basis for this effect, we have studied the consequences of a prolonged incubation with rapamycin on the viability and functions of endothelial cells. Methods and results Human umbilical vein or aorta endothelial cells were exposed to rapamycin in the absence or in the presence of tumour necrosis factor α (TNFα). After a 24 h-incubation, rapamycin (100 nM) caused a significant cell loss associated with the increase of both apoptosis and necrosis, as quantified by propidium iodide staining, caspase 3 activity, and lactate dehydrogenase release. Rapamycin also impair…
Metabolism of propafenone and verapamil by cryopreserved human, rat, mouse and dog hepatocytes: comparison with metabolism in vivo
2003
In the present study we examined the metabolism of [(14)C]propafenone (P) and [(14)C]verapamil (V) using cryopreserved human, dog (Beagle), rat (Sprague-Dawley) and mouse (NMRI) hepatocytes. The percentage ratios of the metabolites were identified after extraction by HPLC with UV and radioactivity detection. Phase-II metabolites were cleaved using beta-glucuronidase. Metabolism of the drugs by cryopreserved hepatocytes was compared with that in the respective species in vivo. All phase-I and -II metabolites known from in vivo experiments: 5-hydroxy-P (5-OH-P); 4'-hydroxy-P (4'-OH-P); N-despropyl-P (NdesP) and the respective glucuronides, were identified after incubation with cryopreserved h…
Effect of two antihypertensive combinations on metabolic control in type-2 diabetic hypertensive patients with albuminuria: a randomised, double-blin…
2001
The objective of this study was to compare, at equal blood pressure (BP) reduction, the effect of two different combinations on metabolic control and albuminuria in type 2 diabetic hypertensive patients with albuminuria. This was a prospective, randomised, double-blind, parallel, controlled trial carried out in 11 Spanish hospitals. A total of 103 type 2 diabetic patients with stable albuminuria and BP not controlled on monotherapy were randomised of which 93 finished the study. After a 4-week single-blind placebo period, patients were randomised to verapamil SR/trandolapril 180/2 mg (VT) or to enalapril/hydroclorothiazide 20/12.5 mg (EH). Treatment duration was 6 months. The main outcome m…
mTOR Driven Gene Transcription Is Required for Cholesterol Production in Neurons of the Developing Cerebral Cortex
2021
AbstractDysregulated mammalian target of rapamycin (mTOR) activity is associated with various neurodevelopmental disorders ranging from idiopathic autism spectrum disorders to syndromes caused by single gene defects. This suggests that maintaining mTOR activity levels in a physiological range is essential for brain development and functioning. Upon activation, mTOR regulates a variety of cellular processes such as cell growth, autophagy and metabolism. On a molecular level, however, the consequences of mTOR activation in the brain are not well understood.Low levels of cholesterol are associated with a wide variety of neurodevelopmental disorders. We here describe numerous genes of the stero…
Cupole e campanili: forme e significato
2012
Cupole e campanili costituiscono elementi fortemente caratterizzanti i nostri panorami urbani, rappresentando spesso la testimonianza tangibile del potere degli ordini religiosi che, attraverso questi elementi di forte impatto visivo, affermano la propria presenza in un determinato contesto. Il contributo mira a offrire una sintesi sul tema delle cupole e dei campanili con riferimento in particolare all'area del trapanese, ricca di testimonianze significative.
Il Tesoro del Collegio dei Gesuiti di Trapani
2012
Nel saggio vengono per la prima volta censiti, analizzati e contestualizzati nella temperie culturale e cronologica di riferimento diciotto manufatti in argento. Si tratta del corredo di suppellettili liturgiche sopravvissute alla soppressione dell'ordine della Compagnia di Gesù di Trapani. Le inedite opere pubblicate sono state prodotte da maestranze siciliane, trapanesi e palermitane, attive tra la fine del XVI secolo e il 1819.
Il turismo a Trapani nel contesto delle destinazioni turistiche siciliane
2006
La provincia di Trapani, nel contesto delle destinazioni turistiche siciliane, rappresenta un punto di riferimento per chi crede che sia possibile sviluppare il turismo puntando sul miglioramento dei servizi di comunicazione e di ricezione offerti al turismo incoming. Il potenziamento dell’aeroporto di Trapani attraverso l’applicazione delle tratte sociali e l’aumento dei collegamenti con gli aeroporti più importanti d’Italia, ha generato un incremento degli arrivi dei passeggeri che ha avuto forti ripercussioni sul movimento turistico verso le destinazioni turistiche della provincia. Notevole è stato, in questi ultimi anni, l’interesse mostrato dagli imprenditori verso la creazione di un p…
« Mouvement est nécessité » Pour une relation au monde du mouvement et de la musique
2021
Starting from a stationary state in order to feed a process, the idea of movement in music can affect both the sound material and the listening (the Hoerspiel...), both the view (musical theater) and the ancillary disciplines (dance or maping-video)... Based on examples taken in the 20th and 21st centuries (Debussy, Berg, Webern, Stravinsky, Stockhausen, Kagel, Ferrari, La Monte Young, Xenakis, Adams, De Chizy, De Mey, Trapani?), musicological study ultimately deduces that the metaphor of movement is for man an experience considered fundamental to his particular relationship to the world.