Search results for "RATES"
showing 10 items of 1636 documents
Cell wall mannoproteins during the population growth phases in Saccharomyces cerevisiae.
1987
Mannoproteins from cell walls of Saccharomyces cerevisiae synthesized at successive stages of the population growth cycle have been solubilized with Zymolyase and subsequently analyzed. The major change along the population cycle concerned a large size mannoprotein material; the size of the newly-synthesized molecules varied from 120,000–500,000 (mean of about 200,000) at early exponential phase to 250,000–350,000 (mean of about 300,000) at late exponential phase. These differences are due to modifications in the amount of N-glycosidically linked mannose residues, since the size of the peptide moiety was 90,000–100,000 at all growth stages and the level of O-glycosylation changed only sligh…
A comparative study of the incorporation of a 1,6-beta-glucan and an O-glycosylated protein epitope into the cell wall of Candida albicans.
1996
The topological distribution of two epitopes in the cell wall of Candida albicans, the kinetics of their incorporation into the regenerating protoplast wall, and the effect of different antibiotics upon their incorporation and localization have been studied. To do so, two monoclonal antibodies that react against an O-glycosylated mannoprotein (1B12) and against a 1,6-beta-glucan epitope (JRR1) were used. The results show that the JRR1 epitope is localized in an internal layer of the cell wall, in contrast to the 1B12 epitope, which is superficial, and that the incorporation of the JRR1 epitope into walls of regenerating protoplasts precedes that of the 1B12 epitope. The JRR1 epitope is norm…
Modeling Volcanic Eruption Parameters by Near-Source Internal Gravity Waves
2016
AbstractVolcanic explosions release large amounts of hot gas and ash into the atmosphere to form plumes rising several kilometers above eruptive vents, which can pose serious risk on human health and aviation also at several thousands of kilometers from the volcanic source. However the most sophisticate atmospheric models and eruptive plume dynamics require input parameters such as duration of the ejection phase and total mass erupted to constrain the quantity of ash dispersed in the atmosphere and to efficiently evaluate the related hazard. The sudden ejection of this large quantity of ash can perturb the equilibrium of the whole atmosphere triggering oscillations well below the frequencie…
GFP-mut2 Proteins in Trehalose-Water Matrixes: Spatially Heterogeneous Protein-Water-Sugar Structures
2007
We report investigations on the properties of nanoenvironments around single-GFP-mut2 proteins in trehalose-water matrixes. Single-GFPmut2 molecules embedded in thin trehalose-water films were characterized in terms of their fluorescence brightness, bleaching dynamics, excited state lifetime, and fluorescence polarization. For each property, sets of approximately 100-150 single molecules have been investigated as a function of trehalose content and hydration. Three distinct and interconverting families of proteins have been found which differ widely in terms of bleaching dynamics, brightness, and fluorescence polarization, whose relative populations sizably depend on sample hydration. The r…
Activity of a Heterogeneous Catalyst in Deep Eutectic Solvents: The Case of Carbohydrate Conversion into 5-Hydroxymethylfurfural
2019
Obtaining industrially relevant products from renewable resources and developing processes under the light of sustainable chemistry is a prominent endeavor of present-day chemical research. In this context, herein we investigated the dehydration of fructose and sucrose in deep eutectic solvents promoted by the ion-exchange resin Amberlyst 15 as heterogeneous catalyst. We optimized the reaction conditions in terms of temperature, catalyst loading, and initial amount of substrate. Then, we looked at the effect of structural variations in the solvent components on the reaction outcome. Under the best experimental conditions, we obtained very good yields of 78% at 60 °C and 69% at 80 °C for fru…
Quantifying Excess Deaths Related to Heatwaves under Climate Change Scenarios: A multicountry time series modelling study
2018
Background: Heatwaves are a critical public health problem. There will be an increase in the frequency and severity of heatwaves under changing climate. However, evidence about the impacts of climate change on heatwave-related mortality at a global scale is limited. Methods and findings: We collected historical daily time series of mean temperature and mortality for all causes or nonexternal causes, in periods ranging from January 1, 1984, to December 31, 2015, in 412 communities within 20 countries/regions. We estimated heatwave–mortality associations through a two-stage time series design. Current and future daily mean temperature series were projected under four scenarios of greenhouse g…
Nutrition Knowledge Is Associated with Energy Availability and Carbohydrate Intake in Young Female Cross-Country Skiers
2021
The aim of this study was to provide information on energy availability (EA), macronutrient intake, nutritional periodization practices, and nutrition knowledge in young female cross-country skiers. A total of 19 skiers filled in weighted food and training logs before and during a training camp. Nutrition knowledge was assessed via a validated questionnaire. EA was optimal in 11% of athletes at home (mean 33.7 ± 9.6 kcal·kgFFM−1·d−1) and in 42% at camp (mean 40.3 ± 17.3 kcal·kgFFM−1·d−1). Most athletes (74%) failed to meet recommendations for carbohydrate intake at home (mean 5.0 ± 1.2 g·kg−1·d−1) and 63% failed to do so at camp (mean 7.1 ± 1.6 g·kg−1·d−1). The lower threshold of the pre-ex…
Smoking cessation opportunities in severe mental illness (tobacco intensive motivational and estimate risk — TIMER—): study protocol for a randomized…
2019
There is an increased risk of premature death in people with severe mental illness (SMI). Respiratory disorders and cardiovascular disease are leading causes of increased mortality rates in these patients, and tobacco consumption remains the most preventable risk factor involved. Developing new tools to motivate patients towards cessation of smoking is a high priority. Information on the motivational value of giving the lung age and prevention opportunities is unknown in this high-risk population. In the context of community care, screening and early detection of lung damage could potentially be used, together with mobile technology, in order to produce a prevention message, which may provi…
Effectiveness of a physical therapeutic exercise programme for caregivers of dependent patients: a pragmatic randomised controlled trial from Spanish…
2020
Producción Científica
Nitrate tolerance as a model of vascular dysfunction: Roles for mitochondrial aldehyde dehydrogenase and mitochondrial oxidative stress
2008
Organic nitrates are a group of very effective anti-ischemic drugs. They are used for the treatment of patients with stable angina, acute myocardial infarction and chronic congestive heart failure. A major therapeutic limitation inherent to organic nitrates is the development of tolerance, which occurs during chronic treatment with these agents. The mechanisms underlying nitrate tolerance remain incompletely defined and are likely multifactorial. One mechanism seems to be a diminished bioconversion of nitroglycerin, another seems to be the induction of vascular oxidative stress, and a third may include neurohumoral adaptations. Recent studies have revealed that mitochondrial reactive oxygen…