Search results for "RBP"

Kvalitātes nodrošināšanas procesu analīze un ieviešana mazā uzņēmumā

2019

Maģistra darba mērķis ir izpētīt, kā mazā uzņēmumā ieviest kvalitātes nodrošināšanas procesu, un veikt tā analīzi. Darbā tiek analizēts esošais kvalitātes nodrošināšanas process un, balstoties un nozares standartiem un labāko praksi, ir izstrādātas jaunas Jira Software darbplūsmas, jeb pieteikumu risināšanas procesi, kuri ir specifiski pielāgota uzņēmuma “Mapon” vajadzībām. Darba ietvaros tika apskatīti vairāki nozares standarti un labās prakses, kas varētu palīdzēt uzņēmumam procesu uzlabošanai. Darba ietvaros tika veikta analīze sākotnējam pieteikumu risināšanas procesam. Balstoties uz iegūto informāciju tika izveidots un analizēts uzlabots pieteikumu risināšanas process. Sākotnējā viena …

RNA-binding proteins expressed in differentiating rat astrocytes

2012

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2008

ROLE OF RNA BINDING PROTEIN IN THE NERVE CELL DIFFERENTIATION

2014

Synthesis of H1˚ and H3.3 histone proteins, in the developing rat brain, seems to be regulated mainly at the post-transcriptional level. Since regulation of RNA metabolism depends on a series of RNA-binding proteins (RBPs), we have been searching for RBPs involved in the post-transcriptional regulation of the H1˚ and H3.3 genes. Previously, we reported isolation, from a cDNA expression library, of an insert encoding a novel protein, the C-terminal half of which is identical to that of PEP-19, a brain-specific protein involved in calcium metabolism. The novel protein was called long PEP-19 isoform (LPI). We showed that LPI, as well as PEP-19, can bind H1˚ RNA. Since PEP19 and LPI contain a c…

Developing rat brain as well as cultured astrocytes contain H1° mRNA-protein complexes

2015

RNA-binding proteins (RBPs) regulate intracellular transport, pre-localization, stability, and translation of mRNAs [1]. We previously identified a set of proteins which interact with mRNAs encoding H1° and H3.3 histones [2-5]. All these proteins are probably part of a ribonucleoprotein particle [6]. Here we report the results of a more detailed study on the expression and intracellular localization of some of these RBPs, such as hnRNP K and A1, and Hsc70, during rat brain development and in cultured rat astrocytes. We also investigated the presence in the complexes of PIPPin/CSD-C2 protein. Affinity chromatography was performed as already described [6]. Preparation of total lysates and cel…

Darba procesa izveide platformā Microsoft SharePoint

2015

Microsoft SharePoint ir saistīts ar lieliem dokumentu apjomiem un dažādu mijiedarbību ar tiem. Lai padarītu darbu ar datiem vieglāku, SharePoint ļauj lietotajiem izveidot darbplūsmas SharePoint Designer programmatūrā. Tomēr, lietotājs var velēties izveidot darbplūsmu, kura nolasa vērtības no PDF formas laukiem. SharePoint Designer ne piedāvā darbplūsmas darbības kuras ir spējīgas to izdarīt. Tomēr, SharePoint dod iespēju lietotajiem pašiem izveidot vajadzīgas darbības un izmantot tas SharePoint dizaineri Kvalifikācijas darbs apraksta “smilškastes” (izolētā) risinājumā izveidi, kas pievieno papildus darbplūsmas darbības SharePoint dizainerī. Projekta mērķis ir izveidot darbplūsmas darbības k…

H1° mRNA-containing complexes in rat brain cells. In: Proceedings of the Abstracts

2015

Post-transcriptional regulation of gene expression depends on RNA-binding proteins (RBPs), which are able to regulate translation, stability and subcellular localization of mRNAs [1]. RNA-protein complexes start to be built up since transcription; some proteins remain then bound to the transcript, while others behave as only transient components. In the developing nervous system of mammals, the postnatal production of the histone variants H1° and H3.3 is mainly regulated at the post-transcriptional level. Synthesis and incorporation into chromatin of the two histone proteins has been suggested to be involved in the epigenetic regulation of gene expression, both in normal brain development a…

Melanoma cells release extracellular vesicle which contain H1° linker histone as well as RNA-binding proteins which bind to the H1° mRNA

2015

We previously demonstrated that G26/24 oligodendroglioma cells release EVs that contain proteins, such as FasL and TRAIL, which induce apoptosis in rat cortical neurons [1] and astrocytes [2]. We also reported that cancer cells use EVs for transferring, into the environment [3], proteins such as extracellular matrix remodelling proteases [4], and H1°, a differentiation-specific histone [5]. In particular, by releasing H1°, cells could escape differentiation cues [5]. To verify the role of EVs in releasing specific proteins and mRNAs, in this study we used as a model A375 melanoma cells. METHODS EVs were purified from cell culture media as previously reported [1, 2]. T1 RNase-protection assa…

Expression and intracellular localization of H1° mRNA-containing complexes in developing rat brain and astrocytes

2015

INTRODUCTION: Post-transcriptional regulation of gene expression relies on RNA-binding proteins (RBPs), which regulate intracellular transport, stability, and translation of mRNAs [1]. We previously identified a set of proteins which interact with mRNAs encoding H1° and H3.3 histones [2-5]. All these proteins are probably part of a ribonucleoprotein particle [6]. Here we report more details on the expression and intracellular localization of some of these RBPs, during rat brain development and in isolated rat astrocytes. METHODS: Affinity chromatography was performed as already described [6]. Preparation of total lysates and cellular sub-fractions was done as reported in [3]. Possible co-lo…

2021

Arginine-glycine(-glycine) (RG/RGG) regions are highly abundant in RNA-binding proteins and involved in numerous physiological processes. Aberrant liquid-liquid phase separation (LLPS) and stress granule (SGs) association of RG/RGG regions in the cytoplasm have been implicated in several neurodegenerative disorders. LLPS and SG association of these proteins is regulated by the interaction with nuclear import receptors, such as transportin-1 (TNPO1), and by post-translational arginine methylation. Strikingly, many RG/RGG proteins harbour potential phosphorylation sites within or close to their arginine methylated regions, indicating a regulatory role. Here, we studied the role of phosphoryla…