Search results for "REGION"
showing 10 items of 4910 documents
Ectopic hbox12 Expression Evoked by Histone Deacetylase Inhibition Disrupts Axial Specification of the Sea Urchin Embryo
2015
Dorsal/ventral patterning of the sea urchin embryo depends upon the establishment of a Nodal-expressing ventral organizer. Recently, we showed that spatial positioning of this organizer relies on the dorsal-specific transcription of the Hbox12 repressor. Building on these findings, we determined the influence of the epigenetic milieu on the expression of hbox12 and nodal genes. We find that Trichostatin-A, a potent and selective histone-deacetylases inhibitor, induces histone hyperacetylation in hbox12 chromatin, evoking broad ectopic expression of the gene. Transcription of nodal concomitantly drops, prejudicing dorsal/ventral polarity of the resulting larvae. Remarkably, impairing hbox12 …
Inhibitory activities of short linear motifs underlie Hox interactome specificity in vivo
2015
Hox proteins are well-established developmental regulators that coordinate cell fate and morphogenesis throughout embryogenesis. In contrast, our knowledge of their specific molecular modes of action is limited to the interaction with few cofactors. Here, we show that Hox proteins are able to interact with a wide range of transcription factors in the live Drosophila embryo. In this context, specificity relies on a versatile usage of conserved short linear motifs (SLiMs), which, surprisingly, often restrains the interaction potential of Hox proteins. This novel buffering activity of SLiMs was observed in different tissues and found in Hox proteins from cnidarian to mouse species. Although th…
Constitutive Promoter Occupancy by the MBF-1 Activator and Chromatin Modification of the Developmental Regulated Sea Urchin α-H2A Histone Gene
2007
The tandemly repeated sea urchin alpha-histone genes are developmentally regulated. These genes are transcribed up to the early blastula stage and permanently silenced as the embryos approach gastrulation. As previously described, expression of the alpha-H2A gene depends on the binding of the MBF-1 activator to the 5' enhancer, while down-regulation relies on the functional interaction between the 3' sns 5 insulator and the GA repeats located upstream of the enhancer. As persistent MBF-1 binding and enhancer activity are detected in gastrula embryos, we have studied the molecular mechanisms that prevent the bound MBF-1 from trans-activating the H2A promoter at this stage of development. Her…
Isolated bladder exstrophy associated with a de novo 0.9 Mb microduplication on chromosome 19p13.12
2012
BACKGROUND: The exstrophy-epispadias complex (BEEC) is a urogenital birth defect of varying severity. The causes of the BEEC are likely to be heterogeneous, with individual environmental or genetic risk factors still being largely unknown. In this study, we aimed to identify de novo causative copy number variations (CNVs) that contribute to the BEEC. METHODS: Array-based molecular karyotyping was performed to screen 110 individuals with BEEC. Promising CNVs were tested for de novo occurrence by investigating parental DNAs. Genes located in regions of rearrangements were prioritized through expression analysis in mice to be sequenced in the complete cohort, to identify high-penetrance mutati…
Sonographic Prognostic Factors in Prenatal Diagnosis of SCT
2003
<i>Objective:</i> A subset of fetuses with sacrococcygeal teratomas (SCT) develops hydrops caused by high-output heart failure. Identification of fetuses at risk for hydrops is important because surgical intervention may reverse the pathophysiology of the disease. The aim of this study was to evaluate sonographic prognostic factors regarding tumor morphology and vascularity associated with the development of hydrops in utero. <i>Methods:</i> Over a 10-year period, we identified 7 fetuses with SCT diagnosed antenatally and managed at the University of Mainz. We retrospectively reviewed the charts of mothers and infants and recorded data on prenatal diagnosis, tumor si…
Cloning of Several Genes Coding for Retinoic Acid Nuclear Receptors in the Mouse Embryonal Carcinoma Cell Line PCC7–MZ1
1993
Mouse embryonal carcinoma cell line PCC7-Mz1 can be induced by retinoic acid (RA) to differentiate into several well defined phenotypes of neuroectodermal origin (Lang, E. et al. (1989) J. Cell. Biol. 109, 2481-2493). Several subclones of the cell line (clonal variants) differ from each other in their developmental potential. To test whether these differences in cellular fate are due to somatic mutations in specific genes of these cells, we have cloned full length cDNAs coding for the alpha 1 and beta 2 isoforms, and partial length cDNAs coding for the alpha 2, beta 1 and beta 3 isoforms of the retinoic acid nuclear receptor (RAR). The cloned cDNAs did not differ in sequence from those of n…
Application of GPS tracking for monitoring spatially unconstrained outdoor recreational activities in protected areas – A case study of ski touring i…
2018
Abstract New trends in leisure and outdoor recreation show an increased penetration of remote areas and the development of spatially unconstrained outdoor recreational activities. Here we present the application of GPS tracking to monitor a spatially unconstrained recreational activity, using ski touring in the Tatra National Park (TNP) as a study case. Particular focus of the study was the spatial and temporal behaviour of ski tourers with regard to existing management measures implemented in the Park, where recreation is limited to designated zones. The research is based upon empirical data (n = 427 ski touring GPS tracks). ArcGIS and statistics software SPSS were used for spatial and sta…
Deviations in pedestrian itineraries in urban areas: a method to assess the role of environmental factors
2010
Walking has long been neglected in urban-mobility research, but it is now making its way into numerous studies using various approaches. Empirical data are often processed in well-known models of flow allocation to study the behaviour of pedestrians and to identify their preferences. However, these models assume that route choices are predetermined at the start of each trip and do not admit any possible intervening decision along these trips. We propose to overcome this limitation through a new method for the analysis of pedestrian behaviour. This method, which we call ‘deviation analysis’ consists of (1) identifying the intersections from which a pedestrian has chosen a route longer than …
Developing cross-industry innovation capability: regional drivers and indicators within firms
2017
ABSTRACTThe role of firms in the process of regional renewal and path development is a somewhat neglected area in the existing literature. With few exceptions, the literature is mainly concerned with aggregated development paths. To cover this gap, the current study turns its attention to cross-industry innovation capability (CIIC) building in firms and discusses how conditions for innovation and learning in a region drive this process. We introduce a new concept of CIIC – that is, the firm’s ability to transform knowledge and ideas from different industries into new products, processes and systems and/or its ability to adapt existing products, processes and systems to new industries – and …
An Exploratory Spatial Data Analysis of European Regional Disparities, 1980–1995
2003
European integration has stimulated numerous studies of regional economic convergence within the European Union in recent macroeconomic and regional science literature (e.g. Abraham and Von Rompuy 1995; Armstrong 1995a; Neven and Gouyette 1995; Martin 2001). Most of the time, the empirical methods that have been used are identical to the methods employed in international studies. However, spatial effects, particularly spatial autocorrelation and spatial heterogeneity, must be taken into account when analyzing the convergence process at regional scale. There are number of factors — trade between regions, technology, knowledge diffusion and more generally regional spillovers — that lead to ge…