Search results for "REPLICATION"
showing 10 items of 489 documents
Structure‐Activity Relationships of Benzamides and Isoindolines Designed as SARS‐CoV Protease Inhibitors Effective against SARS‐CoV‐2
2020
Abstract Inhibition of coronavirus (CoV)‐encoded papain‐like cysteine proteases (PLpro) represents an attractive strategy to treat infections by these important human pathogens. Herein we report on structure‐activity relationships (SAR) of the noncovalent active‐site directed inhibitor (R)‐5‐amino‐2‐methyl‐N‐(1‐(naphthalen‐1‐yl)ethyl) benzamide (2 b), which is known to bind into the S3 and S4 pockets of the SARS‐CoV PLpro. Moreover, we report the discovery of isoindolines as a new class of potent PLpro inhibitors. The studies also provide a deeper understanding of the binding modes of this inhibitor class. Importantly, the inhibitors were also confirmed to inhibit SARS‐CoV‐2 replication in …
Measuring Behavior in the Home Cage: Study Design, Applications, Challenges, and Perspectives
2021
Contains fulltext : 239279.pdf (Publisher’s version ) (Open Access) The reproducibility crisis (or replication crisis) in biomedical research is a particularly existential and under-addressed issue in the field of behavioral neuroscience, where, in spite of efforts to standardize testing and assay protocols, several known and unknown sources of confounding environmental factors add to variance. Human interference is a major contributor to variability both within and across laboratories, as well as novelty-induced anxiety. Attempts to reduce human interference and to measure more "natural" behaviors in subjects has led to the development of automated home-cage monitoring systems. These syste…
Concurrent Computing with Shared Replicated Memory
2019
Any concurrent system can be captured by a concurrent Abstract State Machine (cASM). This remains valid, if different agents can only interact via messages. It even permits a strict separation between memory managing agents and other agents that can only access the shared memory by sending query and update requests. This paper is dedicated to an investigation of replicated data that is maintained by a memory management subsystem, where the replication neither appears in the requests nor in the corresponding answers. We specify the behaviour of a concurrent system with such memory management using concurrent communicating ASMs (ccASMs), provide several refinements addressing different replic…
Case and Contact Linguistics
2012
Abstract Language contact affects case categories in various ways. This article examines the effects of contacts between linguistic codes (languages, unrelated or related, or language varieties): changes in one code on the model of another. It deals with inflectional case markers, affixes, and adpositions from which they evolve. Though most adpositions express more specific relations, some are relatively desemanticised. Affixes and case-like adpositions may fulfil similar functions; the close correspondences between Dravidian case suffixes and Indic postpositions. Case markers and case functions are acquired through what is called ‘borrowing’, ‘diffusion’, ‘transfer’, ‘interference’, ‘repli…
Vortex replication in Bose-Einstein condensates trapped in double-well potentials
2009
In this work we demonstrate, by means of numerical simulations, the possibility of replicating matter-wave vortices in a Bose-Einstein condensate trapped in a double-well potential. The most remarkable result is the generation of replicas of an initial vortex state located in one side of the double potential, which evolves into two copies, each one located in one of the potential minima. A simple linear theory gives the basic explanation of the phenomenon and predicts experimental realistic conditions for observation. A complementary strategy of easy experimental implementation to dramatically decrease the replication time is presented and numerically tested for the general case of nonlinea…
Dynamics and interactions of parvoviral NS1 protein in the nucleus
2007
Summary Nuclear positioning and dynamic interactions of viral proteins with nuclear substructures play essen- tial roles during infection with DNA viruses. Visual- ization of the intranuclear interactions and motility of the parvovirus replication protein (NS1) in living cells gives insight into specific parvovirus protein- cellular structure interactions. Confocal analysis of highly synchronized infected Norden Laboratory Feline Kidney cells showed accumulation of nuclear NS1 in discrete interchromosomal foci. NS1 fused with enhanced yellow fluorescence protein (NS1- EYFP) provided a marker in live cells for dynamics of NS1 traced by photobleaching techniques. Fluo- rescence Recovery after…
Further Evidence that Papillomavirus Capsids Exist inTwo DistinctConformations
2003
ABSTRACT Cell surface heparan sulfate proteoglycans (HSPGs) serve as primary attachment receptors for human papillomaviruses (HPVs). To demonstrate that a biologically functional HPV-receptor interaction is restricted to a specific subset of HSPGs, we first explored the role of HSPG glucosaminoglycan side chain modifications. We demonstrate that HSPG O sulfation is essential for HPV binding and infection, whereas de-N-sulfated heparin interfered with VLP binding but not with HPV pseudoinfection. This points to differences in VLP-HSPG and pseudovirion-HSPG interactions. Interestingly, internalization kinetics of VLPs and pseudovirions, as measured by fluorescence-activated cell sorting analy…
Direct Instruction vs. Cooperative Learning in Physical Education: Effects on Student Learning, Behaviors, and Subjective Experience
2020
(1) Background: The objective was to analyze if cooperative learning (CL) can have benefits compared to direct instruction (DI) in learning, behavior, and subjective experience of pupils. (2) Methods: An intervention was performed with a counterbalanced within-subjects design. To perform the intervention, 75 schoolchildren aged 10 to 12 from four primary classes were divided into two groups, and two units were taught in each one, namely &ldquo
Roflumilast inhibits respiratory syncytial virus infection in human differentiated bronchial epithelial cells.
2013
Respiratory syncytial virus (RSV) causes acute exacerbations in COPD and asthma. RSV infects bronchial epithelial cells (HBE) that trigger RSV associated lung pathology. This study explores whether the phosphodiesterase 4 (PDE4) inhibitor Roflumilast N-oxide (RNO), alters RSV infection of well-differentiated HBE (WD-HBE) in vitro. WD-HBE were RSV infected in the presence or absence of RNO (0.1-100 nM). Viral infection (staining of F and G proteins, nucleoprotein RNA level), mRNA of ICAM-1, ciliated cell markers (digital high speed videomicroscopy, β-tubulin immunofluorescence, Foxj1 and Dnai2 mRNA), Goblet cells (PAS), mRNA of MUC5AC and CLCA1, mRNA and protein level of IL-13, IL-6, IL-8, T…
ICTV Virus Taxonomy Profile: Cystoviridae
2017
The family Cystoviridae includes enveloped viruses with a tri-segmented dsRNA genome and a double-layered protein capsid. The innermost protein shell is a polymerase complex responsible for genome packaging, replication and transcription. Cystoviruses infect Gram-negative bacteria, primarily plant-pathogenic Pseudomonas syringae strains. This is a summary of the International Committee on Taxonomy of Viruses (ICTV) Report on the taxonomy of the Cystoviridae, which is available at http://www.ictv.global/report/cystoviridae.