Search results for "RESISTANCE"
showing 10 items of 3641 documents
Metodo per la genotipizzazione di HIV-1 e relativi kit diagnostici per la rilevazione di farmaco-resistenza (geni GAG/POL).
2007
DESCRIPTION OF THE INVENTION This invention concerns a laboratory method for genotyping human immunodeficiency virus type 1 (HIV-1) through gene sequencing of pol. It allows to detect the nucleotide mutations conferring resistance to antiretroviral drugs active to pol. The present method allows to analyze also low viraemia samples at around 101 -102 copies HIV-RNA/ml. This method offers the advantage of being easily adaptable to both plasma and cellular samples and is therefore able to value "in advance" the genetic mutations potentially involved in the resistance phenomena to anti-retroviral drugs, even in patients with optimal/sub-optimal virologic control. The kit use the one-step RT-PCR…
Expression of IAPs (Inhibitory of Apoptosis Proteins) and of their alternative splice variants in hepatocellular carcinoma tissues and cells
2004
IAPs (inhibitors of apoptosis proteins) might have a major role in the apoptotic resistance that marks many cancers. The studies on IAPs in human HCC have focused on survivin or XIAP, indicating that their new or increased expression in this tumor is associated with a more unfavorable prognosis. The present results corroborate these findings, emphasizing the role that the coordinated expression of different IAPs and alternative splice variants might play in the adverse biology of hepatocellular carcinoma.
Decoding tuberculosis transmission and drug resistance in Valencia Region using whole genome sequencing
2020
La tuberculosis (TB) es una enfermedad infecciosa causada y transmitida por miembros patógenos que pertenecen al complejo de Mycobacterium tuberculosis (MTBC). En el 2018, la Organización Mundial de la Salud (OMS) estimó que 10 millones de personas se infectaron de TB, de los cuales, 1,45 millones de casos murieron a causa de esta. Con estos números, la TB es una de las diez principales causas de muerte en todo el mundo. La TB se transmite por el aire a través de gotas en aerosol generadas al toser por una persona contagiada, por lo que, el sitio de infección más común es el sistema respiratorio. La transmisión es la causa principal de TB en países de alta y baja incidencia. Por este motivo…
Calcestruzzi rinforzati con fibre di PET. Sperimentazioni sull'impiego delle fibre di PET provenienti dalla raccolta differenziata nella produzione d…
2013
La sperimentazione esposta tratta dell’impiego di fibre di PET, ricavate dal semplice taglio di contenitori per bevande, nel rinforzo di calcestruzzi destinati anche a impieghi strutturali. Indaga la durabilità delle fibre in ambiente fortemente basico e le caratteristiche meccaniche di calcestruzzi rinforzati con differenti dosaggi di fibre. Le osservazioni al microscopio elettronico a scansione (SEM) di fibre di PET immerse per 13 giorni a 65°C in acqua distillata e in due soluzioni fortemente basiche mostrano la buona durabilità delle fibre in soluzione 0,103 M di NaOH, che simula la basicità della soluzione acquosa che permea la matrice cementizia. L’influenza delle fibre e del loro dos…
Earth resistance measurements in urban contexts: Problems and possible solutions
2017
Both EN 50522 and IEEE Std. 81 propose the Fall of Potential Method (FPM) to carry out the measurement of the resistance to earth of an Earthing System (ES). However, in urban areas, the recommended distances between the ES and auxiliary electrodes are not easy to respect, due to the presence of buildings and tarmac. Moreover, unknown buried metallic parts, as well as the interconnection among the ESs made by the Distributor System Operator, could modify the earth potential profile of the area, affecting the measurement results. In this paper, the issues that could affect the measurement result if FPM is used in an urban context are presented. A parametric analysis, carried out with Comsol …
Lack of cross‐resistance to otherBacillus thuringiensiscrystal proteins in a population ofPlutella xylostellahighly resistant to cryia(b)
1994
Competition experiments were performed with brush border membrane vesicles of diamondback moth larvae using 125I‐labelled CryIA(b) and unlabelled CryIA(a), CryIA(b) and CryIA(c). The results suggested a model with a single binding site for CryIA(b). Heterologous competition showed that CryIA(c) competed as effectively as CryIA(b) for the CryIA(b) binding site, whereas CryIA(a) competed less effectively. Toxicity tests were performed on third instar larvae with trypsin‐activated insecticidal crystal proteins (ICPs) and a commercial formulation of Bacillus thuringiensis (Bt) (Dipel). A laboratory colony was found to be susceptible to all four ICPs tested and to Dipel. CryIA(b), CryIA(c) and C…
Targeting antibiotic resistant bacteria with phages reduces bacterial density in an insect host
2018
Phage therapy is attracting growing interest among clinicians as antibiotic resistance continues becoming harder to control. However, clinical trials and animal model studies on bacteriophage treatment are still scarce and results on the efficacy vary. Recent research suggests that using traditional antimicrobials in concert with phage could have desirable synergistic effects that hinder the evolution of resistance. Here, we present a novel insect gut model to study phage-antibiotic interaction in a system where antibiotic resistance initially exists in very low frequency and phage specifically targets the resistance bearing cells. We demonstrate that while phage therapy could not reduce th…
Potential Resistance of Employees to Change in the Transition to Industry 5.0
2021
The unprecedented evolution of the technological environment inevitably generates resistance to change in the population. The paper aims to detect these resistances in the conditions of a future transition to Industry 5.0. Firstly, we present the results of a bibliographic study of the specialized literature, in order to answer the dilemma of the existence or absence of the differences between the two concepts, that of Industry 4.0 and that of Industry 5.0. Secondly, the paper contains the results of an online survey based on a questionnaire conducted among employees in the central area of Romania. The research is related to the employees’ perception on the possible evolution of the economi…
Cardiovascular diseases and HCV infection: a simple association or more?
2013
HCV infection, metabolic disorders and cardiovascular alterations, considered alone or in combination, are common conditions in a large proportion of the general population. Consequently, determining whether the association of HCV infection with cardiometabolic disorders is simply coincidental or, conversely, caused by pathogenetic mechanisms (in)directly linking chronic HCV infection to these disorders, would be of extreme relevance. Several clinical studies have shown that metabolic disorders—namely, type 2 diabetes,1 insulin resistance (IR)2 and hepatic steatosis3—are highly prevalent in patients with chronic hepatitis C (CHC) compared with non-infected patients. Experimental and clinica…
Variation in mortality among populations is higher for pymetrozine than for imidacloprid and spiromesifen inTrialeurodes vaporariorumin greenhouses i…
2014
BACKGROUND Insecticide resistance in Trialeurodes vaporariorum W. is unknown in the species' northern distribution range where it inhabits mainly commercial greenhouses. Resistance development in whiteflies feeding on year-round crops in greenhouses is possible owing to the use of chemical treatments to back up biocontrol. The authors tested the response levels to spiromesifen, pymetrozine and imidacloprid in whiteflies collected from seven greenhouses within a 35 km radius in western Finland. RESULTS All except one (PR) population had LC50 values below the recommended concentrations for the tested compounds. However, some populations showed reduced susceptibility to pymetrozine in comparis…