Search results for "REU"
showing 10 items of 1466 documents
Bases moleculares de la actividad señalizadora de dUTPasas
2016
Tesis doctoral: 356 páginas
Discovery of 2-aryl and 2-pyridinylbenzothiazoles endowed with antimicrobial and aryl hydrocarbon receptor agonistic activities
2020
Highlights • Sixteen functionalized benzothiazoles were evaluated as antimicrobial agents and as AhR modulators. • The benzothiazoles showed noticeable antimicrobial effects against Gram-positive and Gram-negative pathogens and against the yeast C. albicans. • Six benzothiazoles exhibited significant AhR agonist effects in a cell-based reporter gene assay. • Structure-activity relationship analysis exposed some relevant headings on the substituent's contributions to the studied biological effects. • Compound 12 displayed promising biocide activity and AhR agonism as well as an adequate ADMET profile and binding similarities with FICZ.
A subunit of eukaryotic translation initiation factor 2α-phosphatase (CreP/PPP1R15B) regulates membrane traffic.
2012
The constitutive reverter of eIF2α phosphorylation (CReP)/PPP1r15B targets the catalytic subunit of protein phosphatase 1 (PP1c) to phosphorylated eIF2α (p-eIF2α) to promote its dephosphorylation and translation initiation. Here, we report a novel role and mode of action of CReP. We found that CReP regulates uptake of the pore-forming Staphylococcus aureus α-toxin by epithelial cells. This function was independent of PP1c and translation, although p-eIF2α was involved. The latter accumulated at sites of toxin attack and appeared conjointly with α-toxin in early endosomes. CReP localized to membranes, interacted with phosphomimetic eIF2α, and, upon overexpression, induced and decorated a pop…
Staphylococcus aureus rezistence pret antimikrobajām vielām un meticilīnrezistences gēna mecA noteikšana
2022
Staphylococcus aureus ir viens no galvenajiem patogēniem, kas spēj izraisīt ādas un mīksto audu infekcijas. Rezistence pret antibiotikām ir saistīta ar plašo virulences faktoru klāstu un tā spēju izmantot horizontālu gēnu pārnesi, it īpaši meticilīnrezistences gēna mecA pārnesi. MRSA celmi vēl joprojām ir izplatīta problēma veselības aprūpē. Darba mērķis: Noteikt Staphylococcus aureus klīnisko izolātu jutību pret antimikrobiālajiem līdzekļiem un mecA gēna klātesamību, izmantojot BBL™ disku difūzijas testu, E-testu un FilmArray ® Multiplex PCR metodi. Rezultāti: Visaugstākā antibakteriālā jutība bija pret rifampicīnu (98,91%), kā arī augsta jutība bija pret cefoksitīnu (96,74%) un trimetopri…
Meticilīnrezistences gēna mecA un toksīna PVL sintēzi noteicošā gēna luk molekulāri bioloģiskā noteikšana Staphylococcus aureus izolātos
2021
Staphylococcus aureus, it īpaši tā meticilīnrezistentie celmi ir vieni no izturīgākajiem mikroorganismiem visā pasaulē, kas pateicoties tiem piemītošajai ģenētiskajai daudzveidībai spēj mainīt sev piederošo virulences faktoru ekspresiju. Tā rezultātā kļūstot par strukturāli un ķīmiski daudzveidīgiem mikroorganismiem, kam ir būtiska loma ar veselības aprūpi saistīto infekciju izplatībā. Mūsdienās, neskatoties uz pēdējos gados vērojamo MRSA celmu izplatības samazināšanos lielākajā daļā ES lielvalstu, arvien biežāk tiek ziņots par pieaugošu Panton-Valentine leikocidīna pozitīvu S. aureus celmu izplatību. Pateicoties, kam tiek sekmēta gan antibakteriālās rezistences attīstība, gan organisma imū…
Elpceļu infekciju gadījumos izdalīto Staphylococcus aureus jutība pret antibakteriālajiem līdzekļiem
2017
S.aureus ir gram + pozitīva baktērija, kas ir lokalizējusies degunā elpošanas traktā un uz ādas un ne vienmer ir patogēns mikroorganisms. Spēj ģēnētiski izmainīt savu šūnapvalka olbaltumvielas, padarot tos rezistentus pret antibiotikām. Meticilīn-rezistentais S. aureus (MRSA) satur mecA gēnu, kas nodrošina mikroorganismam rezistenci ne tikai pret meticilīnu un b - laktāma grupas antibiotikām, bet arī pret cefalosporīniem un citām grupām. Rezistences attīstība patogēniem ir saistīta ar plašu un bieži nepamatotu antibiotiku lietošanu terapijā. Darba mērķis: Noteikt S. aureus jutību pret antibakteriālajiem līdzekļiem, celmiem, kas izdalīti no elpceļu materiāla. Darba uzdevumi: Veikt laboratori…
Comparison of Antibiotic Resistance Profile and Biofilm Production of
2019
Background: The diffusion of antimicrobial resistance is a significant concern for public health worldwide. Staphylococcus aureus represents a paradigm microorganism for antibiotic resistance in that resistant strains appear within a decade after the introduction of new antibiotics. Methods: Fourteen S. aureus isolates from human specimens and twenty-one from samples of animal origin, were compared for their antimicrobial resistance and biofilm capability. In addition, they were characterized at the molecular level to detect the antimicrobial resistance mecA gene and genes related with enterotoxin, toxin, and biofilm production. Results: Both phenotypic and molecular analysis showed main di…
Photoactivated chlorophyllin-based gelatin films and coatings to prevent microbial contamination of food products
2008
[EN] The aim of this work was to develop antimicrobial photosensitizer-containing edible films and coatings based on gelatin as the polymer matrix. incorporating sodium magnesium chlorophyllin (E-140) and sodium copper chlorophyllin (E-141). Chlorophyllins were incorporated into the gelatin film-forming solution and the inhibiting effect of the cast films was tested against Staphylococcus aureus and Listeria monocytogenes. The results demonstrated that water soluble sodium magnesium chlorophyllin and water soluble sodium copper chlorophyllin reduced the growth of S. aureus and L monocytogenes by 5 log and 4 log respectively. Subsequently, the activity of self-standing films and coatings con…
A POLYCARBOXYLIC/AMINO FUNCTIONALIZED HYALURONIC ACID DERIVATIVE FOR THE PRODUCTION OF pH SENSIBLE HYDROGELS IN THE PREVENTION OF BACTERIAL ADHESION …
2014
A graft copolymer derivative of hyaluronic acid bearing pendant amino and short polymethacrylate portions (HA-EDA-BMP-MANa) has been employed for the production of a pH sensible vancomycin releasing hydrogel and studied in vitro to test its potential anti adhesive property against Staphylococcus aureus colonization. The copolymer obtained through atom transfer radical polymerization bears chargeable (carboxyl and amino groups) portions and it could be formulated as a hydrogel at a concentration of 10% w/v. The HA-EDA-BMP-MANa hydrogels, produced at three different pH values (5, 6 and 7, respectively), were formulated with or without the addition of vancomycin (2% w/v). The vancomycin releas…
In vitro antibacterial and biofilm inhibitory activity of the sawdust essential oil of Tetraclinis articulata (vahl) against catheter-associated Stap…
2021
Abstract The aim of the present study was to determine the chemical composition, antibacterial and antibiofilm activities of the essential oil of sawdust from root burl wood of T. articulata. Nine strains including 8 clinical isolates from catheters-related infections and one ATCC 25923 were tested. Twenty-seven different compounds were identified by GC–MS in the essential oil of T. articulata extracted by hydrodistillation. Carvacrol and cedrol were the principal compounds representing 34.37 ± 0.07 and 11.07 ± 0.09 respectively. The EO had a strong antibacterial activity against all strains including one Multidrug-Resistant (MDR) and two Methicillin-Resistant S. aureus strains. Inhibition …