Search results for "RICH"

Fumarate regulation of gene expression in Escherichia coli by the DcuSR (dcuSR genes) two-component regulatory system.

1998

ABSTRACT In Escherichia coli the genes encoding the anaerobic fumarate respiratory system are transcriptionally regulated by C 4 -dicarboxylates. The regulation is effected by a two-component regulatory system, DcuSR, consisting of a sensory histidine kinase (DcuS) and a response regulator (DcuR). DcuS and DcuR are encoded by the dcuSR genes (previously yjdHG ) at 93.7 min on the calculated E. coli map. Inactivation of the dcuR and dcuS genes caused the loss of C 4 -dicarboxylate-stimulated synthesis of fumarate reductase ( frdABCD genes) and of the anaerobic fumarate-succinate antiporter DcuB ( dcuB gene). DcuS is predicted to contain a large periplasmic domain as the supposed site for C 4…

Human histidine-rich glycoprotein expressed in SF9 insect cells inhibits apatite formation

1997

Histidine-rich glycoprotein (HRG) is structurally related to the alpha2-HS glycoprotein/fetuin family of mammalian plasma proteins; both belong to the cystatin superfamily of proteins. We expressed recombinant human HRG and alpha2-HS in Sf9 insect cells for functional analysis. Recombinant HRG bound heparin and fibrinogen while alpha2-HS did not. Both proteins inhibited the formation of apatite, recombinant HRG (IC50 approximately 1 microM) with 2-fold lower molar activity than alpha2-HS (IC50 approximately 0.5 microM). The inhibition in vitro of apatite formation suggests a new function for plasma HRG protein, inhibition of phase separation in blood vessels.

Histidine-rich glycoprotein-induced vascular normalization improves EPR-mediated drug targeting to and into tumors

2018

Tumors are characterized by leaky blood vessels, and by an abnormal and heterogeneous vascular network. These pathophysiological characteristics contribute to the enhanced permeability and retention (EPR) effect, which is one of the key rationales for developing tumor-targeted drug delivery systems. Vessel abnormality and heterogeneity, however, which typically result from excessive pro-angiogenic signaling, can also hinder efficient drug delivery to and into tumors. Using histidine-rich glycoprotein (HRG) knockout and wild type mice, and HRG-overexpressing and normal t241 fibrosarcoma cells, we evaluated the effect of genetically induced and macrophage-mediated vascular normalization on th…

André Richir, Anatomy assistant and moulder at the Alfort veterinary school in the 20th Century

2006

Biography of André Richir (1887-1962), one of the most famous anatomical moulders of the 20th Century, including information on his family, his training, his professional activities and his career at the Alfort veterinary school. An inventory of his works and a description of the techniques that he used, namely for anatomic mouldings.

2021

The intestinal microbiota influences mammalian host physiology in health and disease locally in the gut but also in organs devoid of direct contact with bacteria such as the liver and brain. Extracellular vesicles (EVs) or outer membrane vesicles (OMVs) released by microbes are increasingly recognized for their potential role as biological shuttle systems for inter‐kingdom communication. However, physiologically relevant evidence for the transfer of functional biomolecules from the intestinal microbiota to individual host cells by OMVs in vivo is scarce. By introducing Escherichia coli engineered to express Cre‐recombinase (E. coli Cre) into mice with a Rosa26.tdTomato‐reporter background, …

Functional assays of oxidative stress using genetically engineered Escherichia coli strains.

2003

Oxidative stress may be induced in bacteria by exogenous biocidal agents and is involved in endogenous metabolism. The oxyR operon is a main sensor of oxidative stress and oxyR-deficient bacteria show enhanced sensitivity to oxidative stress and increased accumulation of intracellular reactive oxygen species (ROS). Flow cytometric functional assays in bacteria are limited by the impaired penetration of vital dyes trough the cell wall. Escherichia coli B WP2 strains possess an altered cell-wall lipopolysaccharide that leads to increased membrane permeability. Flow cytometric analysis of WP2 strains is a convenient alternative for cytometric assays of bacterial function. This unit presents pr…

Activation of Human Osteoblasts via Different Bovine Bone Substitute Materials With and Without Injectable Platelet Rich Fibrin in vitro

2021

IntroductionThe aim of the in vitro study was to compare the effect of four bovine bone substitute materials (XBSM) with and without injectable platelet-reach fibrin for viability and metabolic activity of human osteoblasts (HOB) as well as expression of alkaline phosphatase (ALP), bone morphogenetic protein 2 (BMP-2), and osteonectin (OCN).Materials and MethodsCerabone® (CB), Bio-Oss® (BO), Creos Xenogain® (CX) and MinerOss® X (MO) ± i-PRF were incubated with HOB. At day 3, 7, and 10, cell viability and metabolic activity as well as expression of ALP, OCN, and BMP-2, was examined.ResultsFor non-i-PRF groups, the highest values concerning viability were seen for CB at all time points. Pre-t…

El manuscrito del 'Cancionero de Baena' (PN1) : Descripción codicológica y evolución histórica

2018

The present article attempts to establish as accurately as possible the chronological trajectory of the unique codex of the Cancionero de Baena (PN1 in the Dutton nomenclature). It begins with a detailed examination of the codicological aspects of the manuscript, which serve to date its origin to around 1465. This origin, combined with the historical data, supports a conjecture that the manuscript probably belonged to Gonzalo de Beteta, an official of both Enrique IV and the Catholic Kings. It would have passed from him to his grandson, Jorge de Beteta y Cardenas, who gave it to the Real Biblioteca de El Escorial in 1576. The article then follows the vicissitudes of the manuscript from its …

Digitization and preservation of cultural heritage: The CEPROQHA approach

2017

The humanity has always learned from the previous experiences for many reasons. The national heritage proves to be a great way to discover a nation's history. As a result, these priceless cultural items have a special attention. However, Since the wide adoption of new digital technologies, documenting, storing, and exhibiting cultural heritage assets became more affordable and reliable. These digital records are then used in several applications. Researchers saw the opportunity to use digital heritage recordings for long-term preservation. In this paper, we present the research progress in cultural heritage digital processing and preservation, highlighting the most impactful advances. Addit…

Optogenetic Control of Bacterial Expression by Red Light

2022

In optogenetics, as in nature, sensory photoreceptors serve to control cellular processes by light. Bacteriophytochrome (BphP) photoreceptors sense red and far-red light via a biliverdin chromophore and, in response, cycle between the spectroscopically, structurally, and functionally distinct Pr and Pfr states. BphPs commonly belong to two-component systems that control the phosphorylation of cognate response regulators and downstream gene expression through histidine kinase modules. We recently demonstrated that the paradigm BphP from Deinococcus radiodurans exclusively acts as a phosphatase but that its photosensory module can control the histidine kinase activity of homologous receptors.…