Search results for "RICH"

Tiedon alkuperästä : Friedrich Nietzschen naturalistinen epistemologia

2003

Kolme näkemystä väkivallan väistämättömyydestä ihmisyhteisöissä ja luonnossa: Joseph de Maistre, Friedrich Nietzsche ja René Girard

2007

Täytetty Nietzsche : Weimarin Nietzsche-arkisto elämyksellisenä Nietzschelandiana

2016

Preparation of hepatitis C virus structural and non-structural protein fragments and studies of their immunogenicity

2006

Abstract Plasmids pQE-60 and pQE-30 containing 6× His-tag sequence were used for expression of fragments of HCV structural and non-structural proteins in Escherichia coli (E. coli). The following fragments were used: core (1–98 aa), NS3 (202–482 aa), and tetramer of hypervariable region 1 (HVR1) of E2 protein. The constructed plasmids directed high levels of expression of HCV proteins in E. coli JM109. After purification by the metal-affinity chromatography on nickel–nitrilotriacetic acid (Ni–NTA) agarose, the His-tagged HCV proteins were used for immunization of BALB/c mice. All three proteins were able to induce high levels of specific antibodies and, in the case of the NS3 and HVR1 tetra…

A catalytic highly enantioselective direct synthesis of 2-bromo-2-nitroalkan-1-ols through a Henry reaction.

2008

Highly enantiomerically enriched 2-bromo-2-nitroalkan-1-ols are prepared by direct condensation of aliphatic and aromatic aldehydes with bromonitromethane in the presence of a catalytic amount of copper(II) acetate and a C1-symmetric camphorderived amino pyridine ligand. Blay Llinares, Gonzalo, Gonzalo.Blay@uv.es ; Hernandez Olmos, Victor, Victor.Hernandez@uv.es ; Pedro Llinares, Jose Ramon, Jose.R.Pedro@uv.es

A review on some discrete variational techniques for the approximation of essential boundary conditions

2022

We review different techniques to enforce essential boundary conditions, such as the (nonhomogeneous) Dirichlet boundary condition, within a discrete variational framework, and especially techniques that allow to account for them in a weak sense. Those are of special interest for discretizations such as geometrically unfitted finite elements or high order methods, for instance. Some of them remain primal, and add extra terms in the discrete weak form without adding a new unknown: this is the case of the boundary penalty and Nitsche techniques. Others are mixed, and involve a Lagrange multiplier with or without stabilization terms. For a simple setting, we detail the different associated for…

MAST-2D diffusive model for flood prediction on domains with triangular Delaunay unstructured meshes

2011

Abstract A new methodology for the solution of the 2D diffusive shallow water equations over Delaunay unstructured triangular meshes is presented. Before developing the new algorithm, the following question is addressed: it is worth developing and using a simplified shallow water model, when well established algorithms for the solution of the complete one do exist? The governing Partial Differential Equations are discretized using a procedure similar to the linear conforming Finite Element Galerkin scheme, with a different flux formulation and a special flux treatment that requires Delaunay triangulation but entire solution monotonicity. A simple mesh adjustment is suggested, that attains t…

In-depth evaluation of software tools for data-independent acquisition based label-free quantification.

2015

Label-free quantification (LFQ) based on data-independent acquisition workflows currently experiences increasing popularity. Several software tools have been recently published or are commercially available. The present study focuses on the evaluation of three different software packages (Progenesis, synapter, and ISOQuant) supporting ion mobility enhanced data-independent acquisition data. In order to benchmark the LFQ performance of the different tools, we generated two hybrid proteome samples of defined quantitative composition containing tryptically digested proteomes of three different species (mouse, yeast, Escherichia coli). This model dataset simulates complex biological samples con…

Morphological determination of the phototrophic community composition of biological soil crusts in coastal sand dunes in northern Germany

2022

This dataset comprises the microbial community composition of biological soil crusts in north-German sand dunes. For this we obtained enrichment cultures of phototrophic microorganisms, by placing fragments of biocrusts of the same Petri dishes as used for sequencing, in Petri dishes with Bold Basal (1N BBM) agarized medium (Bischoff and Bold 1963). Cultures were grown under standard laboratory conditions: with a 12-hour alteration of light and dark phases and irradiation of 25 μmol photons m-2 s-1 at a temperature 20 ± 5 ºС. Microscopic study of these raw cultures began in the third week of cultivation. Morphological examinations were performed using Olympus BX53 light microscope with Noma…

Phytoplankton strategies and diversity under different nutrient levels and planktivorous fish densities in a shallow Mediterranean lake

2005

Two mesocosm experiments were carried out to investigate the dynamic effects of nutrients (nitrogen and phosphorus) and planktivorous fish additions on phytoplankton strategies and diversity. The phylogenetic and functional approaches were used to understand phytoplankton ecology in shallow Mediterranean lakes. The experimental approach is new for the study of algal functional groups. Nutrient loading and fish stocks enhanced biomass of small algae but decreased phytoplankton diversity and species richness. Faster species replacement and fluctuations in diversity occurred above loadings of 1 μM P and 21 μM N. Mesotrophic conditions favoured a diverse pool of species, including nostocales an…