Search results for "RICH"
showing 10 items of 3360 documents
Two Patients With History of STEC-HUS, Posttransplant Recurrence and Complement Gene Mutations
2013
Hemolytic uremic syndrome (HUS) is a disease of microangiopathic hemolytic anemia, thrombocytopenia and acute renal failure. About 90% of cases are secondary to infections by Escherichia coli strains producing Shiga-like toxins (STEC-HUS), while 10% are associated with mutations in genes encoding proteins of complement system (aHUS). We describe two patients with a clinical history of STEC-HUS, who developed end-stage renal disease (ESRD) soon after disease onset. They received a kidney transplant but lost the graft for HUS recurrence, a complication more commonly observed in aHUS. Before planning a second renal transplantation, the two patients underwent genetic screening for aHUS-associat…
A flux-split algorithm applied to conservative models for multicomponent compressible flows
2003
In this paper we consider a conservative extension of the Euler equations for gas dynamics to describe a two-component compressible flow in Cartesian coordinates. It is well known that classical shock-capturing schemes applied to conservative models are oscillatory near the interface between the two gases. Several authors have addressed this problem proposing either a primitive consistent algorithm [J. Comput. Phys. 112 (1994) 31] or Lagrangian ingredients (Ghost Fluid Method by Fedkiw et al. [J. Comput. Phys. 152 (1999) 452] and [J. Comput. Phys. 169 (2001) 594]). We solve directly this conservative model by a flux-split algorithm, due to the first author (see [J. Comput. Phys. 125 (1996) …
Discovery of the proton emitting nucleus $^{159}$Re
2007
Fund. para Cienc. Tecnol., FCT, Minist. Cienc. Tecnol.;Fundacao Calouste Gulbenkian;Fundacao Luso-Americana
Iron-binding compounds and related outer membrane proteins in Vibrio cholerae non-O1 strains from aquatic environments
1990
A total of 156 strains of Vibrio cholerae non-O1 from aquatic origins were examined for the presence of iron uptake mechanisms and compared with O1 strains and other Vibrio species. All non-O1 strains were able to grow in iron-limiting conditions, with MICs of ethylenediaminedi (O-hydroxyphenylacetic acid) ranging from 20 microM to 2 mM. The production of siderophores was demonstrated by growth in chrome azurol S agar and cross-feeding assays. All strains produced phenolate-type compounds, as assessed by the chemical tests and by bioassays with Salmonella typhimurium enb-7. Some of the strains also promoted the growth of S. typhimurium enb-1 (which can use only enterobactin as a siderophore…
Complete sequence, expression and evolution of two members of the hexamerin protein family during the larval development of the rice moth, Corcyra ce…
2002
Three distinct types of storage hexamerins are expressed in the "last-instar" larvae of the rice moth, Corcyra cephalonica. A cDNA expression library was constructed from fat body-RNA and screened with a polyclonal antibody raised against purified hexamerin (SP2) of Corcyra cephalonica. Two slightly different "full-length" hexamerin cDNA clones (Hex2a and Hex2b) were isolated and sequenced. Both include open reading frames of 2109 bp which are translated into polypeptides of 703 amino acids with 92.5% identity. Signal peptides of 19 amino acids are present at the N-termini. The 684 amino acids native proteins have a high content of aryl groups (17.6%). According to both the criteria for ami…
A novel cell wall protein specific to the mycelial form of Yarrowia lipolytica.
1996
A cDNA clone specifying a cell wall protein was isolated from a Yarrowia lipolytica cDNA library. The cDNA library was constructed in the expression vector lambda gt 11, with the RNA isolated from actively growing mycelial cells. The deduced amino acid sequence shows that the encoded protein contains an N-terminal hydrophobic signal peptide. We have designated this protein YWP1 for Yarrowia lipolytica cell Wall Protein. Northern hybridization identified YWP1 transcript only when Y. lipolytica was growing in the mycelial form. The encoded protein seems to be covalently bound to the glucan cell wall since it is not released from the cell walls by sodium dodecyl sulphate extraction, but it is …
Efficient production of active chicken avidin using a bacterial signal peptide in Escherichia coli
2004
Chicken avidin is a highly popular tool with countless applications in the life sciences. In the present study, an efficient method for producing avidin protein in the periplasmic space of Escherichia coli in the active form is described. Avidin was produced by replacing the native signal sequence of the protein with a bacterial OmpA secretion signal. The yield after a single 2-iminobiotin–agarose affinity purification step was approx. 10 mg/l of virtually pure avidin. Purified avidin had 3.7 free biotin-binding sites per tetramer and showed the same biotin-binding affinity and thermal stability as egg-white avidin. Avidin crystallized under various conditions, which will enable X-ray cryst…
F17-like fimbriae from an invasive Escherichia coli strain producing cytotoxic necrotizing factor type 2 toxin
1994
The F17b fimbriae encoded by the transmissible virulence plasmid Vir, also coding for cytotoxic necrotizing factor type 2, were characterized. A 5.7-kb region of Vir mediates in vitro N-acetylglucosamine-sensitive adhesion to calf intestinal villi. Sequence analysis revealed that this region codes for a structural subunit and an adhesin closely related to the F17-A and F17-G proteins encoded by the F17 fimbrial gene cluster. The F17b-A gene presents an open reading frame of 540 bp encoding a polypeptide of 180 amino acids with a putative signal peptide of 21 residues. The mature protein shows an identity of 74% with the F17-A structural subunit. This 20-kDa protein is recognized by antiseru…
Sustainable Exploitation and Conservation of the Endemic Lake Baikal Sponge (Lubomirskia baicalensis) for Application in Nanobiotechnology
2009
The large sub-continent of Siberia is one of the richest mineral and oil resources on Earth. In its center, one region has gained prominence: Lake Baikal. It is one of the oldest, the deepest, and the lake with the greatest volume on Earth and is inhabited by more than 1,500 endemic species. It was Pallas (1771) who discovered in the lake a sponge species, Lubomirskia baicalensis (Porifera: Demospongiae), which dominates Lake Baikal's littoral-zone benthos. This sponge species has a distinguished, pronounced body plan which is composed of modules. The application of molecular biological and cell biological techniques has allowed an insight into the richness of the genomic regulatory systems…
Boron doping of silicon rich carbides: Electrical properties
2013
Boron doped multilayers based on silicon carbide/silicon rich carbide, aimed at the formation of silicon nanodots for photovoltaic applications, are studied. X-ray diffraction confirms the formation of crystallized Si and 3C-SiC nanodomains. Fourier Transform Infrared spectroscopy indicates the occurrence of remarkable interdiffusion between adjacent layers. However, the investigated material retains memory of the initial dopant distribution. Electrical measurements suggest the presence of an unintentional dopant impurity in the intrinsic SiC matrix. The overall volume concentration of nanodots is determined by optical simulation and is shown not to contribute to lateral conduction. Remarka…