Search results for "RICH"
showing 10 items of 3360 documents
Plasmonic nanosensors reveal a height dependence of MinDE protein oscillations on membrane features
2018
6 p.-4 fig.
Reinstatement of the Patagonian moss Ulota glabella Mitt. (Bryophyta, Orthotrichaceae)
2020
In 1842, J.D. Hooker collected a number of mosses on Hermite Island (Cape Horn region). From one of those gatherings, Hooker 141, four species of Ulota have been described: U. luteola, U. fuegiana, U. glabella, and U. eremitensis. The first two species are widely accepted, whereas the identity of the latter two has been recently discussed, and the names are now synonymized under U. fuegiana, the more widely distributed species in the Tierra del Fuego archipelago. Our studies, based on recent collections of Orthotrichaceae from Patagonia, show that specimens different from those of U. fuegiana and agreeing with the protologues of both U. glabella and U. eremitensis are common in Patagonia. C…
A singular elliptic equation and a related functional
2021
We study a class of Dirichlet boundary value problems whose prototype is [see formula in PDF] where 0 < p < 1 and f belongs to a suitable Lebesgue space. The main features of this problem are the presence of a singular term |u|p−2u and a datum f which possibly changes its sign. We introduce a notion of solution in this singular setting and we prove an existence result for such a solution. The motivation of our notion of solution to problem above is due to a minimization problem for a non–differentiable functional on [see formula in PDF] whose formal Euler–Lagrange equation is an equation of that type. For nonnegative solutions a uniqueness result is obtained.
Pathogenic Escherichia coli Among Asymptomatic Children and Associated Factors
2020
Abstract Although Escherichia coli is a part of the commensal flora of the gastrointestinal tract, pathogenic types of E. coli can cause diarrhea, especially in children. Pathogenic types are found also in healthy individuals, but prevalence of pathogenic E. coli among asymptomatic children varies and has not been studied in Latvia. The aim of the study was to determine prevalence of pathogenic E. coli among asymptomatic children and identify factors associated with presence of bacterium. Children (aged 0.5–8 years) without acute gastrointestinal symptoms were included in a cross-sectional study. Parents were asked to answer a questionnaire (demographic data, parental education, type of del…
Techniques to Analyze sRNA Protein Cofactor Self-Assembly In Vitro
2018
Post-transcriptional control of gene expression by small regulatory noncoding RNA (sRNA) needs protein accomplices to occur. Past research mainly focused on the RNA chaperone Hfq as cofactor. Nevertheless, recent studies indicated that other proteins might be involved in sRNA-based regulations. As some of these proteins have been shown to self-assemble, we describe in this chapter protocols to analyze the nano-assemblies formed. Precisely, we focus our analysis on Escherichia coli Hfq as a model, but the protocols presented here can be applied to analyze any polymer of proteins. This chapter thus provides a guideline to develop commonly used approaches to detect prokaryotic protein self-ass…
Roles for RpoS in survival of Escherichia coli during protozoan predation and in reduced moisture conditions highlight its importance in soil environ…
2017
The soil is a complex ecosystem where interactions between biotic and abiotic factors determine the survival and fate of microbial inhabitants of the system. Having previously shown that Escherichia coli requires the general stress response regulator, RpoS, to survive long term in soil, it was important to determine what specific conditions in this environment necessitate a functional RpoS. This study investigated the susceptibility of soil-persistent E. coli to predation by the single-celled eukaryotes Acanthamoeba polyphaga and Tetrahymena pyriformis, and the role RpoS plays in resisting this predation. Strain-specific differences were observed in the predation of E. coli strains, with so…
Cellular Concentrations of the Transporters DctA and DcuB and the Sensor DcuS of Escherichia coli and the Contributions of Free and Complexed DcuS to…
2017
ABSTRACT In Escherichia coli , the catabolism of C 4 -dicarboxylates is regulated by the DcuS-DcuR two-component system. The functional state of the sensor kinase DcuS is controlled by C 4 -dicarboxylates (like fumarate) and complexation with the C 4 -dicarboxylate transporters DctA and DcuB, respectively. Free DcuS (DcuS F ) is known to be constantly active even in the absence of fumarate, whereas the DcuB-DcuS and DctA-DcuS complexes require fumarate for activation. To elucidate the impact of the transporters on the functional state of DcuS and the concentrations of DcuS F and DcuB-DcuS (or DctA-DcuS), the absolute levels of DcuS, DcuB, and DctA were determined in aerobically or anaerobic…
Successful aerobic bioremediation of groundwater contaminated with higher chlorinated phenols by indigenous degrader bacteria
2018
The xenobiotic priority pollutant pentachlorophenol has been used as a timber preservative in a polychlorophenol bulk synthesis product containing also tetrachlorophenol and trichlorophenol. Highly soluble chlorophenol salts have leaked into groundwater, causing severe contamination of large aquifers. Natural attenuation of higher-chlorinated phenols (HCPs: pentachlorophenol + tetrachlorophenol) at historically polluted sites has been inefficient, but a 4-year full scale in situ biostimulation of a chlorophenol-contaminated aquifer by circulation and re-infiltration of aerated groundwater was remarkably successful: pentachlorophenol decreased from 400 μg L−1 to <1 μg L−1 and tetrachlorophen…
Effect of resource availability on evolution of virulence and competition in an environmentally transmitted pathogen
2018
Understanding ecological and epidemiological factors driving pathogen evolution in contemporary time scales is a major challenge in modern health management. Pathogens that replicate outside the hosts are subject to selection imposed by ambient environmental conditions. Increased nutrient levels could increase pathogen virulence by pre-adapting for efficient use of resources upon contact to a nutrient rich host or by favouring transmission of fast-growing virulent strains. We measured changes in virulence and competition in Flavobacterium columnare, a bacterial pathogen of freshwater fish, under high and low nutrient levels. To test competition between strains in genotype mixtures, we devel…
Soil-transmitted Helminth Infections in Schoolchildren of Laguna de Perlas (Nicaragua).
2016
Soil-transmitted helminth (STH) prevalence, intensity, polyparasitism and co-infections in 425 children from 3 schools of Laguna de Perlas (Nicaragua) were investigated. Single stool samples were analysed by the formalin-ether method and the Kato-Katz. A total of 402 (94.6%) children were infected. Trichuris trichiura 308 (72.4%), Ascaris lumbricoides 115 (27.1%) and Hookworms 54 (12.7%) were the most prevalent STHs. Polyparasitism (322; 75.8%) with two species was most prevalent (109; 25.6%). T. trichiura with A. lumbricoides (19.3%) and T. trichiura with Hookworm (6.8%) were the most common combinations. Positive associations were observed between T. trichiura and A. lumbricoides and T. t…