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RESEARCH PRODUCT
Techniques to Analyze sRNA Protein Cofactor Self-Assembly In Vitro
Valeria MilitelloVéronique ArluisonSergio MarcoSergio MarcoDavid PartoucheDavid PartoucheSylvain TrépoutSylvain TrépoutChristophe SandtThomas BizienMarisela VélezAntoine MalabiradeFrank Wiensubject
0301 basic medicine030103 biophysicsbiologyChemistryNoncoding RNA cofactorComputational biologyNon-coding RNAmedicine.disease_causeIn vitroCofactorProtein self-assembly03 medical and health sciences030104 developmental biologyGene expressionTransfer RNARNA chaperoneFunctional amyloidmedicinebiology.proteinEscherichia colidescription
Post-transcriptional control of gene expression by small regulatory noncoding RNA (sRNA) needs protein accomplices to occur. Past research mainly focused on the RNA chaperone Hfq as cofactor. Nevertheless, recent studies indicated that other proteins might be involved in sRNA-based regulations. As some of these proteins have been shown to self-assemble, we describe in this chapter protocols to analyze the nano-assemblies formed. Precisely, we focus our analysis on Escherichia coli Hfq as a model, but the protocols presented here can be applied to analyze any polymer of proteins. This chapter thus provides a guideline to develop commonly used approaches to detect prokaryotic protein self-assembly, with a special focus on the detection of amyloidogenic polymers.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2018-01-01 |