Search results for "RICH"

showing 10 items of 3360 documents

DECORIN EFFECTS ON PROTEOMIC PROFILING OF BREAST CANCER CELLS: AN UPDATED STUDY

2015

The malignant carcinomas are characterized by several capabilities acquired by the neoplastic cells, among which the ability to invade the extracellular matrix (ECM) and to establish a crosstalk with several ECM components. Under this respect, the extracellular microenvironment is an entity extraordinarily rich of information with opposite signals. Our group has long undertaken the study of the effects of ECM molecules on the behavior of cancer cells in vitro. Among the studied molecules, the decorin was found to exert a non-permissive effect on the growth and motility of the transfected tumor cells. The decorin, belongs to the family of small leucine-rich proteoglycans (SLRP) and is involv…

The malignant carcinomas are characterized by several capabilities acquired by the neoplastic cells among which the ability to invade the extracellular matrix (ECM) and to establish a crosstalk with several ECM components. Under this respect the extracellular microenvironment is an entity extraordinarily rich of information with opposite signals. Our group has long undertaken the study of the effects of ECM molecules on the behavior of cancer cells in vitro. Among the studied molecules the decorin was found to exert a non-permissive effect on the growth and motility of the transfected tumor cells. The decorin belongs to the family of small leucine-rich proteoglycans (SLRP) and is involved physiologically in the fibrillogenesis of collagen. In the last few year a new anti-oncogenic role has been proposed for decorin1. This study aimed to implement the knowledge on the effects of ectopic decorin on breast cancer cells using as a reference point the results already achieved by our research group2 on the experimental model format. By breast cancer cell line 8701-BC and its transfected clone DEC-C2. The extension of the proteomic analysis combined with the mass spectrometry allowed to triplicate the number of identified proteins in our model. Among the newly identified proteins were members of the classes of metabolic enzymes S100 family and cell motility proteins which revealed a net decrease in the decorin transfected cells. Of considerable importance is the observation that these classes of proteins are the most involved in metastatic progression. These results confirm and reinforce the anti-oncogenic role hypothesized for decorin. The work was co-funded by the Italian 5x1000 to COBS.DECORIN
researchProduct

Tommaso Maria Napoli Utriusque Architecturae Compendium Roma 1688 Breve trattato sulle fabbriche civili e militari e la conservazione delle architett…

2013

Il volume Tommaso Maria Napoli Utriusque Architecturae Compendium Roma 1688 Breve trattato sulle fabbriche civili e militari e la conservazione delle architetture del frate domenicano Tommaso Maria Napoli, vuole contribuire ad accrescere le nostre conoscenze sui principi che dovevano sottendere alla realizzazione dell’architettura civile e militare del periodo barocco e tardo barocco, così come espressi nel breve trattato scritto dal frate architetto, nato a Palermo, Tommaso Maria Napoli (1659-1725), e pubblicato a Roma alla fine del Seicento. Alla traduzione dal latino del trattato, redatta da esperti (Fabio Zarbo e Pietro Zarbo), è inclusa un’interessante ricerca, redatta congiuntamente c…

The volume Tommaso Maria Napoli Utriusque Architecturae Brief Compendium 1688 Rome treaty on civil and military factories and preservation of the architecture of the Dominican friar Tommaso Maria Napoli wants to help increase our understanding of the principles that should underpin the realization of civil and military baroque and late baroque period as expressed in the short treatise written by the monk architect born in Palermo Tommaso Maria Napoli (1659-1725) and published in Rome at the end of the seventeenth century. This translation from the Latin of the treaty drawn up by experts (Fabio Zarbo e Pietro Zarbo) included is an interesting research drafted jointly with Giuseppe Tantillo status of some of the architecture without realizing or attributed to the architect Tommaso Maria Napoli. In fact some cards are attached to the volume on the substance and state of preservation of the cathedral of the Assumption of Mary in Dubrovnik (Croatia) of the villas Valguarnera and Palagonia in Bagheria (near Palermo Italy) of the monument to the Immaculate and one of the towers of the church of San Domenico in Palermo (Italy). The volume is enriched by the presentation and afterword by Francesco Tomaselli that among other things starting from the definition of Restoration expressed by Tommaso Maria Napoli in his book presents a stimulating reflection on the meaning of Restoration. The essay Tommaso Maria Napoli Utriusque Architecturae Compendium 1688 ... is the first of the series Monument Document for the conservation of the architectural heritage and environment: Theory knowledge interventions publisher Aracne in Rome with director Francesco Tomaselli and with Comittee Scientific and editorial international.Settore ICAR/19 - Restauro
researchProduct

Thermal and hygrometric properties of traditional calcarenite stones in the area of Palermo

2017

Il contributo espone le misure di conducibilità termica svolte su tre conci di calcarenite conchiliare e la caratterizzazione igrometrica di uno di essi. I risultati sono confrontati con i dati termici e igrometrici riportati dalle norme tecniche esistenti (UNI 10351, UNI EN ISO 10456, UNI EN 1745). The energy improvement of historic buildings requires detailed knowledge of the thermal and hygrometric properties of traditional materials and components. These data should be collected for specific local contexts, where the features of historic constructions are comparable. For the purpose of developing this tool for the architectural heritage of Palermo, this research focuses on calcarenite s…

Thermophysical characterization calcarenite stone thermal conductivity water vapour resistance factor water absorption hygroscopic sorption drying curve porous material capillarity Palermobusiness.industry020209 energy0211 other engineering and technologiesSettore ICAR/10 - Architettura Tecnica02 engineering and technologyMasonryCivil engineeringCalcareniteCaratterizzazione termofisica calcarenite conducibilità termica proprietà igrometriche PalermoArchitectural heritage021105 building & construction0202 electrical engineering electronic engineering information engineeringbusinessGeology
researchProduct

Combining in the melt physical and biological properties of poly(caprolactone) and chlorhexidine to obtain antimicrobial surgical monofilaments.

2012

Bacterial infections on a sutured wound represent a critical problem, and the preparation of suture threads possessing antimicrobial properties is valuable. In this work, poly(caprolactone) (PCL) monofilaments were compounded at the concentration of 1, 2 and 4 % (w/w), respectively, to the antiseptic chlorhexidine diacetate (CHX). The incorporation was carried out in the melt by a single-step methodology, i.e. “online” approach. Mechanical tests revealed that the incorporation of CHX does not significantly change tensile properties of PCL fibres as the thermal profile adopted to prepare the compounded fibres does not compromise the antibacterial activity of CHX. In fact, CHX confers to comp…

Thermoplasticmedicine.drug_classCell SurvivalPolyestersSettore BIO/19 - Microbiologia GeneraleApplied Microbiology and Biotechnologychemistry.chemical_compoundAntisepticTensile StrengthPolymer chemistryUltimate tensile strengthmedicineEscherichia coliHumanschemistry.chemical_classificationpoly(caprolactone)biologyChemistryChlorhexidinechlorhexidineChlorhexidineSuture TechniquesSpectrometry X-Ray EmissionGeneral MedicineFibroblastsbiology.organism_classificationAntimicrobialMicrococcus luteusSettore ING-IND/22 - Scienza E Tecnologia Dei MaterialiEquipment and Suppliessurgical monofilamentsAnti-Infective Agents LocalMicroscopy Electron ScanningMicrococcus luteusAntibacterial activityCaprolactoneBiotechnologyNuclear chemistrymedicine.drugBacillus subtilisApplied microbiology and biotechnology
researchProduct

Thibaut in Italien und ins Italienische. Erste Schritte zu einer Forschungsperspektive

2017

Thibauts RezeptionSettore IUS/18 - Diritto Romano E Diritti Dell'Antichita'Anton Friedrich Justus Thibaut
researchProduct

Thiosulfate Reduction in Salmonella enterica Is Driven by the Proton Motive Force

2012

ABSTRACT Thiosulfate respiration in Salmonella enterica serovar Typhimurium is catalyzed by the membrane-bound enzyme thiosulfate reductase. Experiments with quinone biosynthesis mutants show that menaquinol is the sole electron donor to thiosulfate reductase. However, the reduction of thiosulfate by menaquinol is highly endergonic under standard conditions (Δ E °′ = −328 mV). Thiosulfate reductase activity was found to depend on the proton motive force (PMF) across the cytoplasmic membrane. A structural model for thiosulfate reductase suggests that the PMF drives endergonic electron flow within the enzyme by a reverse loop mechanism. Thiosulfate reductase was able to catalyze the combined …

ThiosulfatesSulfurtransferaseElectron donorNaphtholsBiologyPhotochemistryMicrobiologyGene Expression Regulation Enzymologicchemistry.chemical_compoundElectron transferSulfiteEscherichia coliFormateMolecular BiologyExergonic reactionThiosulfateTerpenesChemiosmosisProton-Motive ForceSalmonella entericaGene Expression Regulation BacterialArticleschemistryBiochemistrySulfurtransferasesThermodynamicsProtonsOxidation-ReductionJournal of Bacteriology
researchProduct

Total Synthesis of the Glycopeptide Recognition Domain of the P-Selectin Glycoprotein Ligand 1

2008

ThreonineGlycosylationGlycosylationOligosaccharidesCatalysischemistry.chemical_compoundSolid-phase synthesisProtein structureAcetamidesHumansChloroacetatesTrichloroacetic AcidBinding siteThreonineAntigens Viral TumorSialyl Lewis X AntigenBinding SitesMembrane GlycoproteinsGlycopeptidesTotal synthesisGeneral ChemistryGlycopeptideProtein Structure TertiaryP-SelectinchemistryBiochemistryP-selectin glycoprotein ligand-1Angewandte Chemie International Edition
researchProduct

Trichinella spiralisThymidylate Synthase: Developmental Pattern, Isolation, Molecular Properties, and Inhibition by Substrate and Cofactor Analogues

1996

Abstract Thymidylate synthase specific activity was found to remain at a constant level in crude extracts from muscle larvae, isolated (1-15 months after infection) by pepsin-HCl digestion, as well as from adult worms ofTrichinella spiralis.The enzyme was purified and its molecular (monomer mol. wt 35 kD) and kinetic (sequential mechanism with the Kmvalues 3.1 and 19 μM for dUMP and N5,10-methylenetetrahydrofolate, respectively) properties determined. 5-Fluoro-dUMP was a competitive, slow-binding inhibitor of the parasite enzyme. N5,10-methylenetetrahydrofolate analogues 10-propargyl-5,8-dideazafolate (CB3717), ZD1694, BW1843U89, and AG337 were weaker inhibitors of the parasite than regener…

Thymidine kinase activityBiophysicsThiophenesBiologyBiochemistryThymidylate synthaseChromatography AffinityGene Expression Regulation EnzymologicCofactorStructure-Activity RelationshipFolic AcidNon-competitive inhibitionFluorodeoxyuridylateAnimalsHumansEnzyme InhibitorsMolecular BiologyTrichinella spiralischemistry.chemical_classificationATP synthaseMusclesGene Expression Regulation DevelopmentalSubstrate (chemistry)Thymidylate SynthaseCell BiologyMolecular biologyLiver RegenerationRatsKineticsEnzymeLiverchemistryBiochemistryLarvaQuinazolinesbiology.proteinSpecific activityBiochemical and Biophysical Research Communications
researchProduct

Chromatogrphic Profiles of Polyphenols and Terpenoids in the Herbs of Some Thymus L. Represntatives

2022

The aim of the work. To perform the identification of phenolic compounds and terpenoids in the aerial parts of Thymus pulegioides L. (cultivar ‘2/6-07’), Thymus richardii Pers. (cultivar 'Fantasy') and Thymus vulgaris L. (cultivar ‘Jalos’) using thin layer chromatography (TLC), as well as to conduct the gaschromatographic analysis of Thymus richardii essential oil. Materials and Methods. The TLC method was used in this study for the qualitative analyses of polyphenols and non-polar compounds in the aerial part of three Thyme (Thymus L.) species from the Lamiaceae Martinov family cultivated in Kherson region (Ukraine). The volatile compounds were determined in the essential oil of Thymus ric…

Thymus pulegioidesThymus vulgarisherbterpenoidsGC/MSTLCThymus richardiipolyphenolscultivarФармацевтичний Часопис
researchProduct

Does low concentration mycotoxin exposure induce toxicity in HepG2 cells through oxidative stress?

2020

The purpose of this study was to determine whether exposure to low concentrations of deoxynivalenol (DON), T-2 toxin (T-2) and patulin (PAT) in a human hepatocellular carcinoma cell line (HepG2) exerts toxic effects through mechanisms related to oxidative stress, and how cells deal with such exposure. Cell viability was determined by the MTT and protein content (PC) assays over 24, 48 and 72 h. The IC

Time FactorsCell SurvivalHealth Toxicology and MutagenesisMitochondria LiverHepatic carcinoma010501 environmental sciencesToxicologymedicine.disease_cause01 natural sciencesPatulinInhibitory Concentration 5003 medical and health scienceschemistry.chemical_compoundmedicineHumansMycotoxinVolume concentration0105 earth and related environmental sciencesMembrane Potential Mitochondrial0303 health sciencesDose-Response Relationship DrugToxinChemistry030302 biochemistry & molecular biologyfood and beveragesHep G2 CellsMycotoxinsMolecular biologydigestive system diseasesOxidative StressT-2 ToxinPatulinHepg2 cellsToxicityHepatocytesLipid PeroxidationReactive Oxygen SpeciesTrichothecenesOxidative stressToxicology Mechanisms and Methods
researchProduct