Search results for "RIMI"

How DNA lesions are turned into powerful killing structures: Insights from UV-induced apoptosis

2008

Mammalian cells treated with ultraviolet (UV) light provide one of the best-known experimental systems for depicting the biological consequences of DNA damage. UV irradiation induces the formation of DNA photoproducts, mainly cyclobutane pyrimidine dimers (CPDs) and (6-4) pyrimidine-pyrimidone photoproducts [(6-4)PPs], that drastically impairs DNA metabolism, culminating in the induction of cell death by apoptosis. While CPDs are the most important apoptosis-inducing lesions in DNA repair proficient cells, recent data indicates that (6-4)PPs also signals for apoptosis in DNA repair deficient cells. The toxic effects of these unrepaired DNA lesions are commonly associated with transcription …

Modulation of Base Excision Repair Alters Cellular Sensitivity to UVA1 but not to UVB¶

2007

Abstract Oxidative DNA damage has been implicated in some of the biological properties of UVA but so far not in the acute photosensitivity or cellular sensitivity. In contrast to pyrimidine dimers, oxidative DNA damage is predominantly processed by base excision repair (BER). In order to further clarify the role of oxidative DNA damage and its repair in the acute cellular response to UV light, we studied UVA1 and UVB sensitivities in three different cell model systems with modified BER. 8-Oxoguanine-DNA-glycosylase 1–/– (OGG1–/–) mouse embryonal fibroblasts and human fibroblasts in which BER was inhibited by incubation with methoxyamine were hypersensitive to UVA1, in particular to low dose…

Experimental and theoretical studies on thymine photodimerization mediated by oxidatively generated DNA lesions and epigenetic intermediates.

2020

[EN] Interaction of nucleic acids with light is a scientific question of paramount relevance not only in the understanding of life functioning and evolution, but also in the insurgence of diseases such as malignant skin cancer and in the development of biomarkers and novel light-assisted therapeutic tools. This work shows that the UVA portion of sunlight, not absorbed by canonical DNA nucleobases, can be absorbed by 5-formyluracil (ForU) and 5-formylcytosine (ForC), two ubiquitous oxidatively generated lesions and epigenetic intermediates present in living beings in natural conditions. We measure the strong propensity of these molecules to populate triplet excited states able to transfer th…

Nuclear expression of apurinic/apyrimidinic endonuclease increases with progression of ovarian carcinomas.

2003

Apurinic/apyrimidinic endonuclease (APE alias Ref-1) is a key enzyme in the base excision repair pathway. Besides its function in DNA repair, APE serves to maintain several transcription factors in an active reduced state such as c-Fos, c-Jun, NF-kappaB, p53 and HIF-1alpha, all of which have been shown to play a role in tumorigenesis. Because of the importance of APE in maintaining genomic stability and gene regulation, we examined whether APE expression is associated with survival and histopathological parameters of patients with ovarian cancer.Tissue sections of primary epithelial ovarian carcinomas from 141 patients were immunostained using a monoclonal antibody directed against APE.Nucl…

Treponema isoptericolens sp. nov., a novel spirochaete from the hindgut of the termite Incisitermes tabogae.

2008

A novel spirochaete, Treponema sp. strain SPIT5T, was isolated from hindgut contents of the drywood termite Incisitermes tabogae (Snyder). The cells of strain SPIT5T were motile, helical in shape, 0.4-0.5 microm in diameter and generally 12-20 microm long. The strain is obligately anaerobic and ferments different mono-, di- and oligosaccharides by forming ethanol as the main liquid fermentation end product. Furthermore, strain SPIT5T was able to grow anaerobically with yeast extract as sole carbon and energy source. Fastest growth was obtained at 30 degrees C, the temperature at which the termites were also grown. The optimum pH for growth was 7.2, with a range of pH 6.5-8.0. The cells poss…

la domesticazione dei bovidi italiani: ipotesi suggerite dallo studio paleogenetico di antichi reperti di Bos primigenius

2008

Nuevos datos biográficos sobre Juan de Miralles

1997

Aportación de nuevos datos biográficos inéditos de Juan Miralles Trayllon, negociante establecido en La Habana en 1740, elemento importante en el tráfico esclavista y agente de España durante los años de la guerra de Emancipación de los EE. UU. de América. Contribution of new unpublished biographical data of Juan Miralles Trayllon, a dealer who was established in La Havana in 1740, important element in the slaveholding traffic and agent of Spain during the years of the Emancipation war of the United States of America.

Nemo teneatur ad impossibile. Las consecuencias de la pragmática para la extirpación del bandolerismo valenciano: cláusulas relativas a la punición d…

2014

Obsesionado con atajar a cualquier precio el problema del bandolerismo, a mediados de 1586 el virrey Aytona publicó en Valencia una pragmática que hacía recaer sobre los dueños de lugares y las autoridades municipales la responsabilidad principal de la lucha contra el crimen. A tenor de las cuantiosas multas que, en aplicación de la misma, se les impondrían durante los 18 años en que la norma estuvo en vigor, en particular por incumplir las cláusulas concernientes al esclarecimiento y sanción de homicidios, cabe concluir que la corona encontró en ella un poderoso instrumento para obligar a los señores y a las oligarquías locales a colaborar más estrechamente en la ardua tarea de asegurar la…

Fattispecie indennitaria e danno da processo penale

2017

L’indennizzo per i danni del processo penale è inquadrato nell’ambito della responsabilità da attività lecita della pubblica amministrazione. Nel diritto amministrativo la concezione “assistenzialistica”, ispirata a un regime di specialità, si affianca a un modello fondato sul paradigma civilistico, con influenze derivanti dalla responsabilità dell’imprenditore da rischio, ovvero dalle molteplici fattispecie di responsabilità oggettiva. Spesso il parametro indennitario si confonde con quello risarcitorio extracontrattuale o precontrattuale. In alcuni casi, gli sviluppi della fattispecie pongono l’accento sulla “tutela dell’affidamento” del privato o sulla «colpa di apparato». Analoghe forme…

Coordinated Interpersonal Behaviour in Collective Dance Improvisation: The Aesthetics of Kinaesthetic Togetherness

2018

International audience; Collective dance improvisation (e.g., traditional and social dancing, contact improvisation) is a participatory, relational and embodied art form which eschews standard concepts in aesthetics. We present our ongoing research into the mechanisms underlying the lived experience of "togetherness" associated with such practices. Togetherness in collective dance improvisation is kinaesthetic (based on movement and its perception), and so can be simultaneously addressed from the perspective of the performers and the spectators, and be measured. We utilise these multiple levels of description: the first-person, phenomenological level of personal experiences, the third-perso…