Search results for "RME"

Saccharomyces cerevisiae signature genes for predicting nitrogen deficiency during alcoholic fermentation

2007

Genome-wide analysis of the wine yeast strain Saccharomyces cerevisiae PYCC4072 identified 36 genes highly expressed under conditions of low or absent nitrogen in comparison with a nitrogen-replete condition. Reverse transcription-PCR analysis for four of these transcripts with this strain and its validation with another wine yeast strain underlines the usefulness of these signature genes for predicting nitrogen deficiency and therefore the diagnosis of wine stuck/sluggish fermentations.

Magnesium alloys laser (Nd:YAG) cladding and alloying with side injection of aluminium powder

2003

International audience; The development of the high purity magnesium alloys (such as WE43) incites the designers to reconsider them, and the car industry appears, these days, the most interested to use this kind of alloy. However, due to their ignitability and tendency to oxidation, magnesium alloys require surface treatments. A series of experiments was carried out in order to improve the corrosion resistance of two types of magnesium alloys, WE43 and ZE41. In most cases, the substrate surface was treated before laser interaction. Side injection of aluminium powder under a 3 kW cw Nd:YAG laser was employed. The deposited layers present very good bond properties. The resulting microstructur…

MoSi2 laser cladding—elaboration, characterisation and addition of non-stabilized ZrO2 powder particles

2003

International audience; The cladding process using laser beam radiation comprises different operational regimes, depending on the involved lasers (usually CO2 or Nd:YAG) and materials. A series of experiments has been carried out to investigate Nd:YAG laser cladding using MoSi2 powder. Procedures and operating parameters for producing clad layers has been developed and their properties evaluated. The feasibility of the laser cladding technique, using a high power Nd:YAG laser, by projecting MoSi2 powder on steel substrate was demonstrated. The results indicate a low density of cracks, elevated powder catchment efficiency (between 65 and 90%) and hardness values around 1200–1300 HV. Our goal…

MoSi2 laser cladding—A new experimental procedure: double-sided injection of MoSi2 and ZrO2

2003

International audience; In the last decade, development of low density advanced material systems for service at temperatures up to 1300 °C was one of the goals of many researches. This kind of material should mainly have moderate fracture toughness and should exhibit oxidation resistant behaviour at low and intermediate temperature. One of the most studied materials continues to be the intermetallic compound MoSi2. The molybdenum disilicide has been considered as an attractive candidate due to its melting point (2030 °C) and excellent oxidation resistance at high temperatures. The main problem associated with the MoSi2 layer synthesized using laser beam is the layer fragility. To avoid this…

Cautionnement. Redressement judiciaire, Absence de déchéance du terme, Renonciation de la caution, Clause non écrite

1995

International audience; (Civ. 1re, 24 janv. 1995, Epoux Lemaire c/ Sté Union bancaire du Nord)

Enzymatic cleaning of inorganic ultrafiltration membranes used for whey protein fractionation

2003

Abstract The aim of this work was to study the cleaning of inorganic membranes fouled by whey protein solutions using proteolytic enzymes. Tami ® 150+4T membranes (Tami Industries, S.A., 26110 Nyons, France) of 400 kg/mol molecular weight cut-off (MWCO) and a ZrO 2 filtering layer were selected to carry out the tests and Maxatase ® XL (Genencor International, 2333 CN Leiden, The Netherlands) and P3-Ultrasil ® 62 (Henkel Iberica, S.A., 08025 Barcelona, Spain) were investigated as cleaning agents. Cleaning efficiency was observed to be a function of the operating conditions. The operating conditions studied were the pH of the cleaning solution, the enzymatic agent concentration and cleaning t…

Permeability and toxicological profile estimation of organochlorine compounds by biopartitioning micellar chromatography

2008

This paper points out the usefulness of biopartitioning micellar chromatography (BMC) as a high-throughput primary screening tool providing key information about the oral absorption, skin permeability (Kp), brain–blood distribution coefficient (BB) and ecotoxicological parameters such as median lethal concentration (LC50) and bioconcentration factors of 15 organochloride compounds. The retention data of compounds in BMC conditions were interpolated in previously developed quantitative–retention activity relationships by our research group. Results show that the compounds studied readily cross the intestinal barrier (oral absorption >ercnt;) and the blood–brain barrier (log BB >p;0.4). In ad…

Chemotactic migration of human diploid fibroblasts is inhibited by contactinhibin.

1992

Strategies for improving production and purification of a recombinant protein: rP30 of Toxoplasma gondii expressed in the yeast Schizosaccharomyces p…

2007

Abstract Many problems concerned with the production and the purification of recombinant proteins must be addressed prior to launching an industrial production process. Among these problems, attention is focused on low-level expression that complicates the purification step and can jeopardise the process. The expression of a membrane protein, rP30, of Toxoplasma gondii in the yeast Schizosaccharomyces pombe led to a secretion of only 0.5 μg ml−1. In order to obtain a sufficient quantity for biochemical characterization and evaluation in vitro diagnostic test development, strategies for both production and purification had to be optimized. First, the influence of four nitrogen sources (three…

The protection of the person in medical research in the Spanish law.

2002

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