6533b856fe1ef96bd12b3285

RESEARCH PRODUCT

Saccharomyces cerevisiae signature genes for predicting nitrogen deficiency during alcoholic fermentation

Ana Mendes-ferreiraAna Mendes-ferreiraElena Jiménez-martíM. Del OlmoJosé García-martínezJosé E. Pérez-ortínArlete Mendes-faiaCecília Leão

subject

Ciências Agrárias::Biotecnologia Agrária e Alimentar:Biotecnologia Agrária e Alimentar [Ciências Agrárias]Saccharomyces cerevisiae ProteinsNitrogenSaccharomyces cerevisiaeGenes FungalSaccharomyces cerevisiaeEthanol fermentationBiologyApplied Microbiology and BiotechnologySaccharomycesGenètica molecular03 medical and health sciencesSaccharomycesGene Expression Regulation Fungal030304 developmental biologyOligonucleotide Array Sequence AnalysisWineGenetics0303 health sciencesScience & TechnologyEcologyModels Genetic030306 microbiologyNitrogen deficiencyReverse Transcriptase Polymerase Chain Reactionfood and beveragesbiology.organism_classificationPhysiology and BiotechnologyYeastYeast in winemakingBiochemistryAlcoholsFermentationFermentationFood ScienceBiotechnology

description

Genome-wide analysis of the wine yeast strain Saccharomyces cerevisiae PYCC4072 identified 36 genes highly expressed under conditions of low or absent nitrogen in comparison with a nitrogen-replete condition. Reverse transcription-PCR analysis for four of these transcripts with this strain and its validation with another wine yeast strain underlines the usefulness of these signature genes for predicting nitrogen deficiency and therefore the diagnosis of wine stuck/sluggish fermentations.

10.1128/aem.01029-07http://dx.doi.org/10.1128/AEM.01029-07