Search results for "RNA processing"
showing 3 items of 63 documents
Evidence for a novel cytoplasmic processing event in ribosome maturation in the sea urchin Paracentrotus lividus
2010
In this work, we demonstrate the existence of a cytoplasmic processing step, never before described, involving both the pre-ribosomal subunits in the sea urchin Paracentrotus lividus. Northern-blot hybridization, primer extension, S1 mapping experiments and in situ hybridizations allowed us to demonstrate that cytoplasmic processed particles are successively re-imported into the nucleus, where maturation of their RNAs is completed prior to being exported to the cytoplasm. Our findings lead to the proposal of a new model of ribosome maturation and shuttling. Moreover, preliminary data from our laboratory suggest that the maturation pathway we propose in P. lividus may not be unique to the se…
Stem Cell-Derived, microRNA-Carrying Extracellular Vesicles: A Novel Approach to Interfering with Mesangial Cell Collagen Production in a Hyperglycae…
2016
Extracellular vesicles (EVs) that are derived from stem cells are proving to be promising therapeutic options. We herein investigate the therapeutic potential of EVs that have been derived from different stem cell sources, bone-marrow (MSC) and human liver (HLSC), on mesangial cells (MCs) exposed to hyperglycaemia. By expressing a dominant negative STAT5 construct (ΔNSTAT5) in HG-cultured MCs, we have demonstrated that miR-21 expression is under the control of STAT5, which translates into Transforming Growth Factor beta (TGFβ) expression and collagen production. A number of approaches have been used to show that both MSC- and HLSC-derived EVs protect MCs from HG-induced damage via the trans…
Nonsense polarity, RNA processing and decay in phage f1.
2011
Nonsense polarity in most cases depends on activation of cryptic transcription terminators. We found that the strong polar effect observed in the nonsense polar mutant R4 of phage f1, mapping in the 5’ proximal region of gene III, instead depends on enhanced instability of mutant mRNAs, whose pattern can be restored by reduction of RNase E activity. rne -(ts) E. coli strains allowed to explore the mechanisms underlying f1 mRNA processing and degradation. The major gene III species, a 1.8 Kb long molecule, appeared to be a secondary transcript, whose decay is modulated by a REP, located at its 3' end. The RNA pool of a mutagenized phage unable to form that structure, lacks completely that tr…