Search results for "RNA."

showing 10 items of 44327 documents

Late Quaternary changes in bat palaeobiodiversity and palaeobiogeography under climatic and anthropogenic pressure: new insights from Marie-Galante, …

2016

25 pages; International audience; Data on Lesser Antillean Late Quaternary fossil bat assemblages remains limited, leading to their general exclusion from studies focusing on Caribbean bat palaeobiodiversity and palaeobiogeography. Additionally, the role of climatic versus human pressure driving changes in faunal communities remains poorly understood. Here we describe a fossil bat assemblage from Blanchard Cave on Marie-Galante in the Lesser Antilles, which produced numerous bat remains from a well-dated, stratified context. Our study reveals the occurrence of at least 12 bat species during the Late Pleistocene and Early Holocene on Marie-Galante, whereas only eight species are currently kn…

0106 biological sciences010506 paleontologyArcheologyPleistoceneAnthropogenic impactWest IndiesContext (language use)010603 evolutionary biology01 natural sciencesCaveGlacial periodEcology Evolution Behavior and SystematicsHolocene[ SDU.STU.PG ] Sciences of the Universe [physics]/Earth Sciences/Paleontology0105 earth and related environmental sciencesGlobal and Planetary Changegeographygeography.geographical_feature_categoryExtinctionFossil chiropteraHoloceneEcologyGeology15. Life on landLate pleistoceneBlanchard CavePalaeoenvironmental changesInterglacialBat fauna turnoverQuaternary[SDU.STU.PG]Sciences of the Universe [physics]/Earth Sciences/PaleontologyGeology
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Diversity of Fish Scales in Late Triassic deposits of Krasiejów (SW Poland)

2018

Abstract. Taxonomy of extinct fishes is mostly based on the shapes of their bodies, teeth and skeletons and sometimes the coverage of the body. Analysis of modern fishes shows that sometimes even single-scale morphology can also be used as a taxonomic tool. In spite of the fact that variation in scales character in one species can be broad, some specific features distinguish species of the same genus. Analysis of the fossilized scales of fishes found in the Late Triassic deposits of Krasiejow (SW Poland) shows that the microstructure of the external surface of scales can also be considered as a taxonomic tool in the fossil record. Description of the ornamentation pattern of several scales o…

0106 biological sciences010506 paleontologyFossil RecordbiologyActinopterygiiActinopterygiiPaleontologybiology.organism_classification010603 evolutionary biology01 natural sciencesPaleontologyornamentationGeographyGenusctenoidTaxonomy (biology)cycloidganoidDipnoiEcology Evolution Behavior and SystematicsSensu stricto0105 earth and related environmental sciencesPaleontological Research
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Comment on the letter of the Society of Vertebrate Paleontology (SVP) dated April 21, 2020 regarding “Fossils from conflict zones and reproducibility…

2020

International audience

0106 biological sciences010506 paleontologyNETHERLANDSARTHROPITYS-BISTRIATAAREACENTRAL-EUROPEPaleontologyPERMIAN PETRIFIED FORESTSAAR-NAHE BASIN010603 evolutionary biology01 natural sciencesArchaeologyPaleontologíaBALTIC AMBEREvoluciónGeographyPARNAIBA BASINSaar–Nahe BasinTHEROPOD DINOSAURBaltic amberDECAPODAVertebrate paleontology[SDU.STU.PG]Sciences of the Universe [physics]/Earth Sciences/PaleontologyComputingMilieux_MISCELLANEOUS0105 earth and related environmental sciences
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Development of Rich Fen on the SE Baltic Coast, Latvia, during the Last 7500 Years, Using Paleoecological Proxies: Implications for Plant Community D…

2016

We present the paleoecological development of a rich fen located in the dune area on the SE Baltic coast, during the last 7500 years. The Apsuciems Mire hosts rare and endangered plant communities in Europe, such as Schoenus ferrugineus and Cladium mariscus. Analysis at high-resolution of plant macroremains in two peat cores was carried out to reconstruct local vegetation succession and fluctuations in moisture availability on the peatland, while a pollen record was developed to reconstruct plant succession, moisture variability and human activity at the regional scale. Based on the presence or the absence of macroremains of plants that occur in wet habitat e.g. Cladium mariscus, Schoenople…

0106 biological sciences010506 paleontologygeographyPeatgeography.geographical_feature_categoryEcologyCladium mariscusbiologyEcologyWetlandPlant communityEcological successionVegetationbiology.organism_classification010603 evolutionary biology01 natural sciencesEnvironmental Science(all)MireEnvironmental ChemistryEnvironmental scienceSchoenoplectus tabernaemontani0105 earth and related environmental sciencesGeneral Environmental ScienceWetlands
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Lowering histamine formation in a red Ribera del Duero wine (Spain) by using an indigenous O. oeni strain as a malolactic starter

2016

This study demonstrates for the first time that a non-commercial selected autochthonous O. oeni strain has been used to conduct malolactic fermentation (MLF) while lowering histamine formation in the same winery. Lactic acid bacteria (LAB) were isolated from 13 vats before and after spontaneous MLF at the Pago de Carraovejas winery from the Ribera del Duero region (Spain). Only O. oeni were present, typed and characterized, and both histamine producer and non-producers existed. From the non-producers, one strain was selected to become a starter according to its genetic profile, prevalence in the different wines in the winery, resistance to alcoholic degree, resistance to high polyphenolic c…

0106 biological sciences0301 basic medicine030106 microbiologyMalatesWine01 natural sciencesMicrobiology03 medical and health scienceschemistry.chemical_compoundStarterMalate DehydrogenaseRNA Ribosomal 16S010608 biotechnologyMalolactic fermentationLactic AcidFood scienceOenococcusWinebiologyStrain (chemistry)food and beveragesGeneral Medicinebiology.organism_classificationWineryRandom Amplified Polymorphic DNA TechniqueLactic acidchemistrySpainFermentationFood MicrobiologyHistamineBacteriaHistamineFood ScienceInternational Journal of Food Microbiology
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Effects of acclimation time and epigenetic mechanisms on growth of Neurospora in fluctuating environments

2017

AbstractReaction norms or tolerance curves have often been used to predict how organisms deal with fluctuating environments. A potential drawback is that reaction norms measured in different constant environments may not capture all aspects of organismal responses to fluctuating environments. We examined growth of the filamentous fungusNeurospora crassain fluctuating temperatures and tested if growth in fluctuating temperatures can be explained simply by growth in different constant temperatures or if more complex models are needed. In addition, as previous studies on fluctuating environments have revealed that past temperatures that organisms have experienced can affect their response to c…

0106 biological sciences0301 basic medicineAcclimatizationMutantEnvironmentMethylation010603 evolutionary biology01 natural sciencesAcclimatizationNeurosporaArticleEpigenesis GeneticNeurospora crassaHistones03 medical and health sciencesGeneticsEpigeneticsGenetics (clinical)030304 developmental biology0303 health sciencesbiologyCell CyclefungiTemperatureAcetylationDNA MethylationModels Theoreticalbiology.organism_classificationFilamentous fungusNeurospora030104 developmental biologyRNA Interference PathwayH3k4 methylationDNA methylationBiophysicsGene-Environment InteractionRNA Interference
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Highly efficient construction of infectious viroid-derived clones

2019

[Background] Viroid research generally relies on infectious cDNA clones that consist of dimers of the entire viroid sequence. At present, those dimers are generated by self-ligation of monomeric cDNA, a strategy that presents several disadvantages: (i) low efficiency, (ii) it is a non-oriented reaction requiring tedious screenings and (iii) additional steps are required for cloning into a binary vector for agroinfiltration or for in vitro RNA production.

0106 biological sciences0301 basic medicineAgroinfiltrationIIs enzymesViroidvirusesPlant ScienceComputational biologylcsh:Plant cultureBiology01 natural sciences03 medical and health sciencesAgro-infiltrationComplementary DNAGeneticsLethal allelelcsh:SB1-1110Vector (molecular biology)Dimerslcsh:QH301-705.5CloningViroidMethodologyRNAbiology.organism_classificationRestriction enzyme030104 developmental biologylcsh:Biology (General)010606 plant biology & botanyBiotechnologyCloning
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Ocean acidification at a coastal CO2 vent induces expression of stress-related transcripts and transposable elements in the sea anemone Anemonia viri…

2019

Published version, available at: https://doi.org/10.1371/journal.pone.0210358 Ocean acidification threatens to disrupt interactions between organisms throughout marine ecosystems. The diversity of reef-building organisms decreases as seawater CO2 increases along natural gradients, yet soft-bodied animals, such as sea anemones, are often resilient. We sequenced the polyA-enriched transcriptome of adult sea anemone Anemonia viridis and its dinoflagellate symbiont sampled along a natural CO2 gradient in Italy to assess stress levels in these organisms. We found that about 1.4% of the anemone transcripts, but only ~0.5% of the Symbiodinium sp. transcripts were differentially expressed. Processe…

0106 biological sciences0301 basic medicineAtmospheric ScienceMolecular biologyMarine and Aquatic SciencesGene ExpressionRetrotransposonSea anemone01 natural sciencesAnemoniaSequencing techniquesMobile Genetic ElementsMultidisciplinarybiologyQREukaryotaOcean acidificationAnemoneRNA sequencingGenomicsChemistryRetrotransposonsPhysical SciencesMedicineTranscriptome AnalysisResearch ArticleScienceZoology010603 evolutionary biology03 medical and health sciencesGreenhouse GasesCnidariaGenetic ElementsSea WaterGeneticsVDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470Environmental ChemistryAnimalsMarine ecosystemBiology and life sciencesEcology and Environmental SciencesDinoflagellateChemical CompoundsOrganismsTransposable ElementsCorrectionAquatic EnvironmentsComputational BiologyCarbon Dioxidebiology.organism_classificationGenome AnalysisMarine EnvironmentsInvertebratesVDP::Mathematics and natural science: 400::Basic biosciences: 470Research and analysis methods:Genetikk og genomikk: 474 VDP::Marinbiologi:497 VDP::Økologi:488 [VDP]030104 developmental biologySea AnemonesMolecular biology techniquesAtmospheric ChemistryEarth SciencesSeawater
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Host dispersal shapes the population structure of a tick-borne bacterial pathogen

2020

Birds are hosts for several zoonotic pathogens. Because of their high mobility, especially of longdistance migrants, birds can disperse these pathogens, affecting their distribution and phylogeography. We focused on Borrelia burgdorferi sensu lato, which includes the causative agents of Lyme borreliosis, as an example for tick-borne pathogens, to address the role of birds as propagation hosts of zoonotic agents at a large geographical scale. We collected ticks from passerine birds in 11 European countries. B. burgdorferi s.l. prevalence in Ixodes spp. was 37% and increased with latitude. The fieldfare Turdus pilaris and the blackbird T. merula carried ticks with the highest Borrelia prevale…

0106 biological sciences0301 basic medicineBORRELIA-BURGDORFERIACARImedicine.disease_causemigrationBURGDORFERI SENSU-LATO01 natural sciencesSongbirdsLyme diseaseTicksAcariMigration11832 Microbiology and virologyLyme DiseasebiologyPlan_S-Compliant_NOBLACKBIRDS TURDUS-MERULAPREVALENCEEuropehost-parasite interactionsMIGRATORY BIRDSinternational1181 Ecology evolutionary biology[SDE]Environmental SciencesHost-paraste InterationsHost-parasite interactionsAvesTRANSMISSIONZoology010603 evolutionary biologyticksBirds03 medical and health sciencesBorreliaparasitic diseasesGeneticsmedicineAnimalsHumansBorrelia burgdorferiEcology Evolution Behavior and SystematicsLyme borreliosisInfecções Sistémicas e ZoonosesIxodesBird DiseasesBorreliaLYME-DISEASEIXODES-RICINUS TICKSBorrelia gariniiEcologíabiology.organism_classificationmedicine.diseasebacterial infections and mycoses030104 developmental biologybirdsCandidatusWILD BIRDSMultilocus sequence typingBorrelia gariniiIxodesMultilocus Sequence Typing
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A Metabarcoding Survey on the Fungal Microbiota Associated to the Olive Fruit Fly.

2017

The occurrence of interaction between insects and fungi is interesting from an ecological point of view, particularly when these interactions involve insect pests and plant pathogens within an agroecosystem. In this study, we aimed to perform an accurate analysis on the fungal microbiota associated to Bactrocera oleae (Rossi) through a metabarcoding approach based on 454 pyrosequencing. From this analysis, we retrieved 43,549 reads that clustered into 128 operational taxonomic units (OTUs), of which 29 resulted in the “core” associate fungi of B. oleae. This fungal community was mainly represented by sooty mould fungi, such as Cladosporium spp., Alternaria spp. and Aureobasidium spp., by pl…

0106 biological sciences0301 basic medicineBactrocera oleaeOlive fruit flySoil ScienceAureobasidium01 natural sciences03 medical and health sciencesMicrobial ecologyPseudocercospora454 PyrosequencingAscomycotaOleaBotanyColletotrichumAnimalsDNA Barcoding TaxonomicPseudocercosporaEcology Evolution Behavior and SystematicsEcological nicheHigh-throughput sequencingEcologybiologyBase SequenceEcologyfungiTephritidaefood and beveragesSettore AGR/12 - Patologia VegetaleAlternariaHigh-Throughput Nucleotide SequencingSequence Analysis DNAbiology.organism_classificationAlternaria030104 developmental biologySettore AGR/11 - Entomologia Generale E ApplicataColletotrichumDNA Intergenic454 Pyrosequencing; Bactrocera oleae; Cladosporium; Colletotrichum; High-throughput sequencing; PseudocercosporaCladosporium010606 plant biology & botanyCladosporiumMycobiomeMicrobial ecology
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