Search results for "RNAi"
showing 10 items of 41 documents
Intra-eruptive gas emissions and shallow magma storage after the 2007 summit caldera collapse of Piton de la Fournaise, Reunion island
2012
The tumor suppressor p14ARF hampers proliferation of aneuploid cells induced by CENP-E partial depletion
The Spindle Assembly Checkpoint (SAC) is a cellular surveillance mechanism that ensures faithfully segregation of chromosomes. Reduced expression of some of its components weakens the SAC and induces chromosome instability and aneuploidy, both hallmarks of tumor cells. Centromere Protein-E (CENP-E) is a crucial component of the SAC and facilitates kinetochore microtubule attachment required to achieve and maintain chromosome alignment. To investigate the possible role of p14ARF on aneuploid cells proliferation we induced aneuploidy in primary human fibroblasts (IMR90) and in near diploid tumor cells (HCT116) by partial depletion of CENP-E obtained by RNA interference. Our results show that …
"Ultra vestimenta seu ornamenta ecclesiastica que etiam dedi eidem ecclesie". Tracce di un'eredità palermitana dell'arcivescovo Jean de Carondelet (1…
2016
Lo studio, dopo una breve biografia aggiornata di Jean Carondelet, importante colto ecclesiastico e politico fiammingo, che fu anche arcivescovo di Palermo dal 1520 al 1544, ne indaga la committenza artistica (vetrate istoriate, paramenti sacri ricamati, stalli corali lignei intagliati, manoscritti liturgici miniati, dipinti, sculture marmoree) tra Franca Contea, Paesi Bassi borgognoni e la stessa Palermo, col supporto di un'inedito testamento secondo cui egli avrebbe donato già in vita "vestimenta seu ornamenta ecclesiastica" di grande valore alla cattedrale palermitana, tra cui forse la mitria e il paliotto già tradizionalmente identificati col suo cognome.
Kalmbergin kartasto R VI : List 7
1855
A novel antiviral approach.
2012
Viral infections are often the etiological agents of severe acute and chronic human diseases. Their peculiar biology usually leads to the need of design specific therapies for each virus, and the eradication of the viruses and the healing of the patients very often are not reached also after decades of theoretical and applied researches. HIV is a classical example of how the efforts of the researchers may be disappointed in eradicating a virus infection in an infected patient. Here I present a hypothesis for a new antiviral approach that may be suitable for the treatment of HIV infected patients. The same approach, with opportune modifications, may be also applied as healing strategy for a …
Efficient selection of silenced primary cells by flow cytometry
2007
Background: RNA interference has emerged as a new and potent tool to knockdown the expression of target genes and to investigate their functions. For short time experiments with mammalian cell lines, RNA interference is typically induced by transfecting small interfering RNAs (siRNAs). Primary cells constitute important experimental systems in many studies because of their similarity to their in vivo counterparts; however, transfection of these cells has been found to be difficult. As a consequence, RNA interference of primary cells may result in mixed phenotypes because of the simultaneous presence in the same preparation of transfected and nontransfected cells. This may be particularly in…
RNAi mediated acute depletion of Retinoblastoma protein (pRb) promotes aneuploidy in human primary cells via micronuclei formation
2009
BACKGROUND: Changes in chromosome number or structure as well as supernumerary centrosomes and multipolar mitoses are commonly observed in human tumors. Thus, centrosome amplification and mitotic checkpoint dysfunctions are believed possible causes of chromosomal instability. The Retinoblastoma tumor suppressor (RB) participates in the regulation of synchrony between DNA synthesis and centrosome duplication and it is involved in transcription regulation of some mitotic genes. Primary human fibroblasts were transfected transiently with short interfering RNA (siRNA) specific for human pRb to investigate the effects of pRb acute loss on chromosomal stability. RESULTS: Acutely pRb-depleted fibr…
Morphologic variation in the P1 element of Mississippian species of the conodont genus Pseudognathodus
2018
A revision of the Austin Conodont Collection at the Natural History Museum, London, has revealed a diverse assemblage of P1 elements of Pseudognathodus . Pseudognathodus homopunctatus, Ps. lineatus, Ps. mermaidus and Ps. symmutatus are di?erentiated and a new species, Ps. posadachaconae , is de?ned from material from North Wales. The systematic of these species is updated. A revised diagnosis is provided for the genus Pseudognathodus , as well as for the species Ps. lineatus and Ps. symmutatus . The types of Ps. symmutatus cannot be differentiated from immature specimens of Ps. homopunctatus . The evolutionary relationships of Pseudognathodus species are discussed. Stratigraphic distributio…
Étude de deux formations carbonifères du Morvan méridional dans le cadre de l'orogenèse hercynienne (Région du Creusot, Saône-et-Loire).
2006
18 pages; National audience; Dans la région creusotine, deux formations sédimentaires, l'une d'un âge incertain, Dévonien, Dévono-Dinantien, Dinantien, selon les auteurs, et l'autre du Stéphanien supérieur, ont été étudiées. La première nommée Formation (Fm) des Alouettes, est bordée au nord par le granite d'Uchon responsable d'un métamorphisme de contact. Des filons de microgranite la recoupent. Elle est d'abord constituée par une alternance de grès et de siltites laminées charbonneuses dans lesquelles on rencontre des rides de courant, figures d'érosion ainsi que des racines de Lépidophytes (Stigmaria) et des tiges d'Equisétales (Bornia). Cette partie est recouverte par des conglomérats à…
Catàleg de cavallers i religiosos de l’Orde de Montesa (1592-1701)
2014
Catàleg de cavallers i religiosos de l’Orde de Montesa (1592-1701) The goal of this research was to identify the members of the Order of Montesa in the seventeenth century, based on the situation of the religious and military order at the time when it was united with the Crown in 1592 and its subsequent evolution throughout the whole of the following century. The result was the identification through individualized records of the 431 knights, 205 monks and 25 bearded friars of the Order of Montesa in the seventeenth century.