Search results for "RNAi"
showing 10 items of 41 documents
Le Quaternaire franc-comtois. Essai chronologique et paléoclimatique.
1982
Le but de ce travail est d'établir le cadre chronologique et paléoclimatique du Quaternaire en Franche-Comté à partir de l'étude des formations édifiées au cours de cette période.Deux grands types de dépôts ont été plus particulièrement étudiés: ·- Les remplissages karstiques d'avens, de porches de grottes et d'abris sous roche, donnant un éclairage intense, mais ponctuel, de l'environnement physique et biologique, grâce aux faunes abondantes et aux indices climatiques d'ordre sédimentologique.- Les dépôts glaciaires et paraglaciaires (moraines, fluvioglaciaires, glacio-lacustres) qui permettent de situer des coupures climatiques au cours du temps dans l'espace géographique étudié.L'étude d…
First results of the Piton de la Fournaise STRAP 2015 experiment: multidisciplinary tracking of a volcanic gas and aerosol plume
2016
Abstract. The STRAP (Synergie Transdisciplinaire pour Répondre aux Aléas liés aux Panaches volcaniques) campaign was conducted in 2015 to investigate the volcanic plumes of Piton de La Fournaise (La Réunion, France). For the first time, measurements at the local (near the vent) and at the regional scales around the island were conducted. The STRAP 2015 campaign has become possible thanks to a strong cross-disciplinary collaboration between volcanologists and meteorologists. The main observations during four eruptive periods (85 days) are summarized. They include the estimates of SO2, CO2 and H2O emissions, the altitude of the plume at the vent and over different areas of La Réunion Island, …
Inducing Cold-Sensitivity in the Frigophilic Fly Drosophila montana by RNAi
2016
The work was supported by CNPq (Fellowship to FMV) and a NERC Studentship to DJP. Cold acclimation is a critical physiological adaptation for coping with seasonal cold. By increasing their cold tolerance individuals can remain active for longer at the onset of winter and can recover more quickly from a cold shock. In insects, despite many physiological studies, little is known about the genetic basis of cold acclimation. Recently, transcriptomic analyses in Drosophila virilis and D.montana revealed candidate genes for cold acclimation by identifying genes upregulated during exposure to cold. Here, we test the role of myo-inositol-1-phosphate synthase (Inos), in cold tolerance in D. montana …
Cell biology: Refined siRNA screens.
2012
A large set of high-content RNAi screens investigating mammalian virus infection and multiple cellular activities is analysed to reveal the impact of population context on phenotypic variability and to identify indirect RNAi effects.
IP 3 signalling regulates exogenous RNA i in C aenorhabditis elegans
2015
RNA interference (RNAi) is a widespread and widely exploited phenomenon. Here, we show that changing inositol 1,4,5-trisphosphate (IP3) signalling alters RNAi sensitivity in Caenorhabditis elegans. Reducing IP3 signalling enhances sensitivity to RNAi in a broad range of genes and tissues. Conversely up-regulating IP3 signalling decreases sensitivity. Tissue-specific rescue experiments suggest IP3 functions in the intestine. We also exploit IP3 signalling mutants to further enhance the sensitivity of RNAi hypersensitive strains. These results demonstrate that conserved cell signalling pathways can modify RNAi responses, implying that RNAi responses may be influenced by an animal's physiology…
Dystroglycan regulates structure, proliferation and differentiation of neuroepithelial cells in the developing vertebrate CNS.
2007
AbstractIn the developing CNS α- and β-dystroglycan are highly concentrated in the endfeet of radial neuroepithelial cells at the contact site to the basal lamina. We show that injection of anti-dystroglycan Fab fragments, knockdown of dystroglycan using RNAi, and overexpression of a dominant-negative dystroglycan protein by microelectroporation in neuroepithelial cells of the chick retina and optic tectum in vivo leads to the loss of their radial morphology, to hyperproliferation, to an increased number of postmitotic neurons, and to an altered distribution of several basally concentrated proteins. Moreover, these treatments also altered the oriented growth of axons from retinal ganglion c…
Mariamas Petrosjanas romāna "Māja, kurā..." žanra poētika.
2016
Anna Kļimjuka Mariamas Petrosjanas romāna "Māja, kurā..." žanra poētika: maģistra darbs. – Rīga, 2016. - 76 lpp. Šīs pētījums ir veltīts mūsdienu Marjamas Petrosjanas romāna “Māja, kurā…” žanra poēti-kai. Pētijuma mērķis ir identificēt mūsdienu romāna nozīmes un uzbūves pamatprincipus gan literārā žanra ietvaros, gan Marjamas Petrosjanas literārajā darbā. Darbs ir sadalīts četrās daļās. Pirmajā daļā ir iezīmētie galvenie teorijas un praktikas jautā-jumi par romāna žanra kanonisko izpratni; otrajā, trešajā un ceturtajā daļās – saskaņā ar izvirzītiem mērķim tiek identificētas un parādītas mūsdienu romāna transformācijas iezīmes, romāna kinematografitātes, kā arī postfeminsma probēmas atspoguļ…
Data from: Quantitative PCR primer design affects quantification of dsRNA-mediated gene knockdown
2020
RNA interference (RNAi) is a powerful tool for studying functions of candidate genes in both model and non-model organisms and a promising technique for therapeutic applications. Successful application of this technique relies on the accuracy and reliability of methods used to quantify gene knockdown. With the limitation in the availability of antibodies for detecting proteins, quantitative PCR (qPCR) remains the preferred method for quantifying target gene knockdown after dsRNA treatment . We evaluated how qPCR primer binding site and target gene expression levels affect quantification of intact mRNA transcripts following dsRNA-mediated RNAi. The use of primer pairs targeting the mRNA sequ…
Single-cell analysis of population context advances RNAi screening at multiple levels
2012
Isogenic cells in culture show strong variability, which arises from dynamic adaptations to the microenvironment of individual cells. Here we study the influence of the cell population context, which determines a single cell's microenvironment, in image‐based RNAi screens. We developed a comprehensive computational approach that employs Bayesian and multivariate methods at the single‐cell level. We applied these methods to 45 RNA interference screens of various sizes, including 7 druggable genome and 2 genome‐wide screens, analysing 17 different mammalian virus infections and four related cell physiological processes. Analysing cell‐based screens at this depth reveals widespread RNAi‐induce…
Vusja žanrs ķīniešu modernajā literatūrā un kino
2017
Bakalaura darba tēma ir „Vusja žanrs ķīniešu modernajā literatūrā un kino.” Vusja ir ķīniešu daiļliteratūras un kino žanrs, kas balstīts uz cīņas mākslu un fantastikas elementiem. Vusja kino ar fantastikas elementiem savu sākotnējo izcelsmi ir radis ķīniešu daiļliteratūrā.Darba mērķis ir noskaidrot vusja žanra iezīmes ķīniešu modernajā literatūrā un kino. Darba gaitā tiek analizēti literatūras avoti, apskatīti vusja žanra raksturojošie elementi un noskaidrotas vusja žanra iezīmes ķīniešu modernajā literatūrā un kino. Bakalaura darbs sastāv no trīs daļām. Pirmajā daļā tiek skaidrots vusja jēdziens ķīniešu kultūrā, noskaidroti žanra aizsākumi un tā raksturojošie elementi. Otrā daļa iepazīstin…