Search results for "RULE"
showing 10 items of 1403 documents
Bacteriophage Adherence to Mucus Mediates Preventive Protection against Pathogenic Bacteria
2019
The mucosal surfaces of animals are habitat for microbes, including viruses. Bacteriophages—viruses that infect bacteria—were shown to be able to bind to mucus. This may result in a symbiotic relationship in which phages find bacterial hosts to infect, protecting the mucus-producing animal from bacterial infections in the process. Here, we studied phage binding on mucus and the effect of mucin on phage-bacterium interactions. The significance of our research is in showing that phage adhesion to mucus results in preventive protection against bacterial infections, which will serve as basis for the development of prophylactic phage therapy approaches. Besides, we also reveal that exposure to m…
Staphylococcus epidermidis virulences faktori un epidemioloģiskā nozīme
2016
Staphylococcus epidermidis svarīgākais virule nces faktors ir biofilmas veidošana. Lai diferencētu komensālos celmus no kolonizējošiem un invazīviem celmiem, pētījumā tika izmantotas fenotipiskās metodes, nosakot biofilmas veidošanu, antibiogrammu, un molekulārās bioloģijas metodes, nosakot virulences gēnus aap / ica A un mec A. Rezultāti liecina, ka biofilmu veidošana raksturīga klīniskajiem celmiem, statistiski ticami biežāk gēni aap/ica A bija noteikti klīniskajos S.epidermidis celmos, tie vis biežāk bija rezistenti pret meticilīnu (97,8%). S.epidermidis tipēšanai pirmo reizi tika pielietota MLST (multilokusu sekvenču tipēšana) metode. Pētījuma rezultāti ļauj secināt par S.epidermidis ge…
Mitochondrial cannabinoid receptors gate corticosterone impact on novel object recognition
2023
: Corticosteroid-mediated stress responses require the activation of complex brain circuits involving mitochondrial activity, but the underlying cellular and molecular mechanisms are scantly known. The endocannabinoid system is implicated in stress coping, and it can directly regulate brain mitochondrial functions via type 1 cannabinoid (CB1) receptors associated with mitochondrial membranes (mtCB1). In this study, we show that the impairing effect of corticosterone in the novel object recognition (NOR) task in mice requires mtCB1 receptors and the regulation of mitochondrial calcium levels in neurons. Different brain circuits are modulated by this mechanism to mediate the impact of cortico…
New Modalities in Knee Osteoarthritis Treatment Using Autologous Bone Marrow-Derived Mononuclear Cells
2018
Abstract The clinical effects on knee osteoarthritis (OA) symptoms and tissue structure were evaluated after bone marrow-derived mononuclear cell intraarticular injection. A group of 32 patients with 34 knee joints in stage II–III osteoarthritis were treated by intraarticular injection of mononuclear cell suspension. Clinical results were obtained by KOOS (Knee Osteoarthritis Outcome Score) and KSS (Knee Society Score) scores during a 12 months follow-up period. Radiological evaluation was performed using magnetic resonance imaging. A comparison with a control group of 28 patients treated with routinely used three hyaluronic acid intra-articular injections was made. No adverse effects were …
Taking the acid test: Mediterranean limpets face up to climate change
2014
Impacts of climate change involve the interactions of multiple stressors on intertidal organisms, but rarely are the impacts of these stressors examined together. Many tests are also conducted in artificial, controlled laboratory conditions, without making use of natural opportunities to test performance of organisms under different environmental stressors. Given its small tidal range, the Mediterranean Sea provides such an opportunity, with a very fine scale environmental gradient and species living very close to each other over the tidal gradient. The vertical distribution of the limpets, Patella rustica and P. caerulea overlap in Palermo, Sicily, but despite this they have different ther…
General Aspects Regarding the Situations when the Rules of Good Conduct in Scientific Research Can Become Offences
2017
Abstract The purpose of the paper is to clarify the situations when deviations from the rules of good conduct in scientific research are considered to be offenses. The paper, in the first part, it is focused on the idea of offence and it definition, because it is important to define this term in order to compare deviations and offences. In the second part, the paper refers to those situations when the deviations from the rules of good conduct in scientific research can become offences according to the Criminal Code. In addition, in the paper are analyse the regulation from art. 21 Law no. 206/2004 and some examples of situations when deviations can became offences. To complete this picture …
Certifcazione ambientale di prodotti agroalimentari LCA dell’olio d’oliva
2009
Oggi viene ampiamente riconosciuta l’importanza di valutare gli impatti ambientali connessi alla produzione dei prodotti agroalimentari. L’applicazione della LCA (Life Cycle Assessment) per la valutazione degli impatti ambientali generati dall’intero ciclo di vita del prodotto risulta particolarmente complessa nel campo alimentare. Ciò a causa delle numerose variabili in gioco, connesse, ad esempio, ai sistemi di coltivazione, specie nell’ottica della definizione delle PCR (Product Category Rules) per la redazione della EPD (Environmental Product Declaration). Il presente studio LCA, effettuato su un olio extravergine d’oliva ottenuto da olive prodotte in Sicilia con sistema di coltivazione…
Hydrological simulation using a bivariate approach based on copulas for the assessment of inundation risk scenarios down-stream of flood control rese…
2021
Aim of this study was to assess operational reservoir rules for evaluating flood risk scenarios using a MonteCarlo bivariate framework. It involves a stochastic procedure to calculate flood hydrographs and, then, flood inundation area evaluation through a 2D hydraulic model to estimate the inundation risk and, as consequence, the relative damage evaluation, under different operation strategies of an upstream reservoir. The entire procedure can be summarised as follows: single synthetic stochastic rainfall events have been derived using a Monte Carlo proce-dure through a bivariate copulas analysis; synthetic bivariate stochastic inflow hydrographs have been derived by using a conceptual full…
A novel intrinsically disordered outer membrane lipoprotein ofAggregatibacter actinomycetemcomitansbinds various cytokines and plays a role in biofil…
2017
Intrinsically disordered proteins (IDPs) do not have a well-defined and stable 3-dimensional fold. Some IDPs can function as either transient or permanent binders of other proteins and may interact with an array of ligands by adopting different conformations. A novel outer membrane lipoprotein, bacterial interleukin receptor I (BilRI) of the opportunistic oral pathogen Aggregatibacter actinomycetemcomitans binds a key gatekeeper proinflammatory cytokine interleukin (IL)-1b. Because the amino acid sequence of the novel lipoprotein resembles that of fibrinogen binder A of Haemophilus ducreyi, BilRI could have the potential to bind other proteins, such as host matrix proteins. However, from th…
Evolutionary Dynamics of Pathoadaptation Revealed by Three Independent Acquisitions of the VirB/D4 Type IV Secretion System in Bartonella
2017
The α-proteobacterial genus Bartonella comprises a group of ubiquitous mammalian pathogens that are studied as a model for the evolution of bacterial pathogenesis. Vast abundance of two particular phylogenetic lineages of Bartonella had been linked to enhanced host adaptability enabled by lineage-specific acquisition of a VirB/D4 type IV secretion system (T4SS) and parallel evolution of complex effector repertoires. However, the limited availability of genome sequences from one of those lineages as well as other, remote branches of Bartonella has so far hampered comprehensive understanding of how the VirB/D4 T4SS and its effectors called Beps have shaped Bartonella evolution. Here, we repor…