Search results for "Rat brain"

Long survival time of an isolated perfused rat brain

1972

Nicotinamide adenine dinucleotides in the convulsant rat brain

1970

Quantitative analysis of the EEG effects produced by imipramine, desipramine, promazine, and monodesmethyl promazine in the isolated perfused rat bra…

1974

The effects of imipramine, desipramine, promazine and monodesmethyl promazine on the EEG of the isolated perfused rat brain were studied. The brain preparation was perfused for 30 min with simulated blood, containing of the drugs in a concentration of 10−5 M. Control experiments were performed without a drug added to the simplified blood. The EEG was recorded at various times on a magnetic tape and was evaluated visually and quantitatively (amplitude and interval histography). The EEG effects of imipramine and promazine as well as the effects of these drugs with their monodesmethyl metabolites were compared. The drugs produced clear EEG changes compared with the control EEG. An increase of …

Uptake and storage of choline by rat brain: influence of dietary choline supplementation.

1991

In order to elucidate the regulation of the levels of free choline in the brain, we investigated the influence of chronic and acute choline administration on choline levels in blood, CSF, and brain of the rat and on net movements of choline into and out of the brain as calculated from the arteriovenous differences of choline across the brain. Dietary choline supplementation led to an increase in plasma choline levels of 50% and to an increase in the net release of choline from the brain as compared to a matched group of animals which were kept on a standard diet and exhibited identical arterial plasma levels. Moreover, the choline concentration in the CSF and brain tissue was doubled. In th…

Steady state concentrations of clomipramine and its major metabolite desmethylclomipramine in rat brain and serum after oral administration of clomip…

2000

Twenty male Sprague-Dawley rats received five oral doses of clomipramine 20 mg/kg at 4-h intervals. The animals were decapitated 1, 2, 3, 5 and 12 h after the last dose for determination of clomipramine and desmethylclomipramine in serum and frontal cerebral cortex. Time dependent concentrations of clomipramine and desmethylclomipramine paralleled in serum and brain. Half-lives were similar in serum and brain with 7.8 versus 6.2 h and 5.5 versus 5.0 h for clomipramine and desmethylclomipramine, respectively. Absolute concentrations, however, were markedly higher in brain than in serum - 12.5 fold for clomipramine and 7.4 fold for desmethylclomipramine. The data indicate that serum and brain…

Identification of calcium sensing receptor (CaSR) mRNA-expressing cells in normal and injured rat brain

2009

Calcium sensing receptor (CaSR), isolated for the first time from bovine and human parathyroid, is a G-protein-coupled receptors that has been involved in diverse physiological functions. At present a complete in vivo work on the identification of CaSR mRNA-expressing cells in the adult brain lacks and this investigation was undertaken in order to acquire more information on cell type expressing CaSR mRNA in the rat brain and to analyse for the first time its expression in different experimental models of brain injury. The expression of CaSR mRNAs was found mainly in scattered cells throughout almost all the brain regions. A double labeling analysis showed a colocalization of CaSR mRNA expr…

Aspartate aminotransferase and carbohydrate metabolism of rat brain

1969

E stato determinato il rapporto tra forma «piridossalica» e forma «piridossaminica» dell'aspartato aminotransferasi nell'encefalo totale di ratti albini durante l'ipoglicemia da insulina e l'iperglicemia da allossana. Il rapporto tra le due forme catalitiche dell'enzima e stato considerato un indice dell'efficienza del ciclo degli acidi tricarbossilici, ed e stata prospettata la possibilita che l'aspartato aminotransferasi abbia una funzione regolativa nei riguardi del ciclo.

Lactate Dehydrogenase Isozymes in the Developing Rat Brain

1962

THE immaturity and the lack of differentiation of the rat brain at birth has been shown some fifty years ago by Sugita1 in a classic paper on the postnatal morphogenesis of the brain in this animal. The sequence of physiological and biochemical changes which parallel the histological maturation of the rat brain has been investigated, however, only recently.

Quantitative Topography of the Hemodynamic-Oxidative Metabolic Interrelation by Multispot Measurement Approach in Rat Ischemia Models

2001

We applied the multispot measurement approach for both laser Doppler (LD) and a microspectrophotometric method to measure local cerebral blood flow (1CBF) and local tissue hemoglobin oxygenation (IHbO2). We demonstrated that the current technology permits in vivo observation, allowing us to establish a close topographical relation between CBF and HbO2. We measured 1CBF by LD flowmetry and tissue HbO2 by a microspectrophotometric method at multiple corresponding locations using a “scanning” technique that employs a computer-controlled micromanipulator. CBF and HbO2 data from predefined locations were correlated to the topographical situation and then arranged in a three-dimensional (3D) imag…

Fingolimod (FTY720-P) Does Not Stabilize the Blood–Brain Barrier under Inflammatory Conditions in an in Vitro Model

2015

Breakdown of the blood-brain barrier (BBB) is an early hallmark of multiple sclerosis (MS), a progressive inflammatory disease of the central nervous system. Cell adhesion in the BBB is modulated by sphingosine-1-phosphate (S1P), a signaling protein, via S1P receptors (S1P\(_1\)). Fingolimod phosphate (FTY720-P) a functional S1P\(_1\) antagonist has been shown to improve the relapse rate in relapsing-remitting MS by preventing the egress of lymphocytes from lymph nodes. However, its role in modulating BBB permeabilityin particular, on the tight junction proteins occludin, claudin 5 and ZO-1has not been well elucidated to date. In the present study, FTY720-P did not change the transendotheli…