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showing 10 items of 22191 documents

Inter- and intraspecific variability in invertebrate acute toxicity response to Arsenic and Fluoride exposure

2018

The adverse effects of arsenic and fluoride exposure on six groups of freshwater invertebrates were investigated. Acute toxicity tests (48-h) with arsenic trioxide (As2NO3) resulted in the following pattern of sensitivity: Daphnia magna 24-h-old = Brachionus patulus 72-h-old = Daphnia. cf. prolata, 21-d-old = D. magna 5-d-old > Heterocypris incongruens juvenile instars > Culex sp. Heterocypris juv. incongruens instars were the second group more tolerant to arsenic and the second group that bioconcentrates arsenic the least. In contrast, invertebrates exposed to sodium fluoride (NaF), showed a different pattern of sensitivity: H. incongruens instars > B. patulus = D. magna 24-h-old > D. cf. …

0301 basic medicine030102 biochemistry & molecular biologyfungiZoologychemistry.chemical_elementBiologyAcute toxicityIntraspecific competition03 medical and health scienceschemistry.chemical_compound030104 developmental biologychemistryZoologiaFluorideInvertebrats d'aigua dolçaArsenicArsènic ToxicologiaInvertebrate
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Cellular Concentrations of the Transporters DctA and DcuB and the Sensor DcuS of Escherichia coli and the Contributions of Free and Complexed DcuS to…

2017

ABSTRACT In Escherichia coli , the catabolism of C 4 -dicarboxylates is regulated by the DcuS-DcuR two-component system. The functional state of the sensor kinase DcuS is controlled by C 4 -dicarboxylates (like fumarate) and complexation with the C 4 -dicarboxylate transporters DctA and DcuB, respectively. Free DcuS (DcuS F ) is known to be constantly active even in the absence of fumarate, whereas the DcuB-DcuS and DctA-DcuS complexes require fumarate for activation. To elucidate the impact of the transporters on the functional state of DcuS and the concentrations of DcuS F and DcuB-DcuS (or DctA-DcuS), the absolute levels of DcuS, DcuB, and DctA were determined in aerobically or anaerobic…

0301 basic medicine030106 microbiologyBiologymedicine.disease_causeMicrobiologyDNA-binding proteinMass Spectrometry03 medical and health sciencesFumaratesTranscriptional regulationmedicineEscherichia coliDicarboxylic AcidsAnaerobiosisPhosphorylationMolecular BiologyTranscription factorEscherichia coliDicarboxylic Acid TransportersCatabolismKinaseEscherichia coli ProteinsAutophosphorylationGene Expression Regulation BacterialAerobiosisDNA-Binding Proteins030104 developmental biologyBiochemistryPhosphorylationProtein KinasesSignal TransductionTranscription FactorsResearch ArticleJournal of bacteriology
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Antiviral properties of silver nanoparticles against norovirus surrogates and their efficacy in coated polyhydroxyalkanoates systems

2017

Silver nanoparticles (AgNP) have strong broad-spectrum antimicrobial activity and gained increased attention for the development of AgNP based products, including medical and food applications. Initially, the efficacy of AgNP and silver nitrate (AgNO3) was evaluated for inactivating norovirus surrogates, the feline calicivirus (FCV) and the murine norovirus (MNV). These norovirus surrogates were exposed to AgNO3 and AgNP solutions for 24 h at 25 °C and then analyzed by cell-culture assays. Both AgNP and silver ions significantly decreased FCV and MNV infectivity in a dose-dependent manner between concentrations of 2.1 and 21 mg/L. Furthermore, poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (…

0301 basic medicine030106 microbiologyved/biology.organism_classification_rank.speciesNoroviruses02 engineering and technologyengineering.materialSilver nanoparticlePolyhydroxyalkanoatesMicrobiology03 medical and health scienceschemistry.chemical_compoundCoatingFiberFeline calicivirusbiologyElectrospinningved/biologyChemistryPolyhydroxyalkanoates021001 nanoscience & nanotechnologybiology.organism_classificationElectrospinningSilver nitrateengineeringActive packagingSilver nanoparticles0210 nano-technologyFood ScienceMurine norovirusNuclear chemistry
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Adolescent Feeding: Nutritional Risk Factors

2018

AbstractThe aim of this review is to describe adolescent nutritional requirements from a scientific, nutritional, and psychosocial point of view, emphasizing the main risks of malnutrition at this age. Currently, too many teenagers are alone at home and prepare their own meals. The influence of friends replaces parental rules. The habits of adolescents are characterized by a tendency to skip meals, especially breakfast, and, less frequently, the midday meal; to purchase carry-out meals away from home; to consume snacks, especially sweets; to eat at fast food restaurants, almost always with high-calorie content; and to eat fried foods, soft drinks, and sweets. Toxic habits, such as smoking, …

0301 basic medicine030109 nutrition & dieteticsCaloriebusiness.industrySaturated fatdigestive oral and skin physiologyPhysical activitymedicine.diseaseHealthy diet03 medical and health sciencesMalnutritionEnvironmental healthPediatrics Perinatology and Child HealthmedicineSurgeryMidday mealbusinessNutritional riskPsychosocialJournal of Child Science
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2021

Proteins play a crucial role in metabolism, in maintaining fluid and acid-base balance and antibody synthesis. Dietary proteins are important nutrients and are classified into: 1) animal proteins (meat, fish, poultry, eggs and dairy), and, 2) plant proteins (legumes, nuts and soy). Dietary modification is one of the most important lifestyle changes that has been shown to significantly decrease the risk of cardiovascular (CV) disease (CVD) by attenuating related risk factors. The CVD burden is reduced by optimum diet through replacement of unprocessed meat with low saturated fat, animal proteins and plant proteins. In view of the available evidence, it has become acceptable to emphasize the …

0301 basic medicine030109 nutrition & dieteticsNutrition and DieteticsCholesterolbusiness.industrySaturated fatfood and beverages030209 endocrinology & metabolismCritical Care and Intensive Care Medicinemedicine.diseaseLower riskObesity03 medical and health scienceschemistry.chemical_compound0302 clinical medicineInsulin resistancechemistryWeight lossmedicineRed meatFood scienceMetabolic syndromemedicine.symptombusinessClinical Nutrition
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Environmental radiation alters the gut microbiome of the bank vole Myodes glareolus.

2018

International audience; Gut microbiota composition depends on many factors, although the impact of environmental pollution is largely unknown. We used amplicon sequencing of bacterial 16S rRNA genes to quantify whether anthropogenic radionuclides at Chernobyl (Ukraine) impact the gut microbiome of the bank vole Myodes glareolus. Exposure to elevated levels of environmental radionuclides had no detectable effect on the gut community richness but was associated with an almost twofold increase in the Firmicutes:Bacteroidetes ratio. Animals inhabiting uncontaminated areas had remarkably similar gut communities irrespective of their proximity to the nuclear power plant. Hence, samples could be c…

0301 basic medicine16STechnologymetsämyyräFirmicutessuolistomikrobisto030106 microbiologyMicrobial metabolismgut microbiomeZoologyFirmicutesEnvironmental pollutionGut floraBrief CommunicationMicrobiologysäteilybiologia03 medical and health sciencesMicrobial ecologyRNA Ribosomal 16SGeneticsAnimalsMicrobiomebank voleLife Below WaterEcology Evolution Behavior and Systematics[SDV.EE]Life Sciences [q-bio]/Ecology environmentRibosomalbiologyBacteriaBacteroidetesArvicolinaeBacteroidetessäteilyBiological Sciencesbiology.organism_classificationGastrointestinal MicrobiomeBank vole030104 developmental biologyChernobyl Nuclear Accident13. Climate actionenvironmental radionuclidesRNACarbohydrate MetabolismEnvironmental SciencesRadioactive Pollutants
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Decline in the incidence of colorectal cancer and the associated mortality in young Italian adults

2020

Objective The incidence of colorectal cancer (CRC) declines among subjects aged 50 years and above. An opposite trend appears among younger adults. In Europe, data on CRC incidence among younger adults are lacking. We therefore aimed to analyse European trends in CRC incidence and mortality in subjects younger than 50 years. Design Data on age-related CRC incidence and mortality between 1990 and 2016 were retrieved from national and regional cancer registries. Trends were analysed by Joinpoint regression and expressed as annual percent change. Results We retrieved data on 143.7 million people aged 20–49 years from 20 European countries. Of them, 187 918 (0.13%) were diagnosed with CRC. On a…

0301 basic medicine2312ColonPopulationSocio-culturalecolorectal cancercolorectal cancer screening03 medical and health sciencesYoung Adult0302 clinical medicineEpidemiology of cancerMedicineHumans1506Risk factoreducationeducation.field_of_studycancer epidemiology Colorectal cancer colorectal cancer screeningbusiness.industryIncidence (epidemiology)Mortality ratescreeningIncidenceGastroenterologyCancermedicine.diseaseObesityAnnual Percent ChangeEurope030104 developmental biologyItaly030211 gastroenterology & hepatologyepidemiologybusinessColorectal NeoplasmsDemographycancer epidemiologySEER Program
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Hybrid Biopolymer and Lipid Nanoparticles with Improved Transfection Efficacy for mRNA

2020

Cells 9(9), 2034 (1-19) (2020). doi:10.3390/cells9092034

0301 basic medicine570small angle scatteringNanoparticlecationic lipid02 engineering and technologyengineering.materialTransfectionArticleCell LineFatty Acids Monounsaturated03 medical and health sciencesMiceBiopolymersddc:570AnimalsHumansRNA MessengerParticle Sizelcsh:QH301-705.5chemistry.chemical_classificationMice Inbred BALB Ccancer immunotherapySmall-angle X-ray scatteringHeparinOptical ImagingCationic polymerizationGeneral MedicinePolymerTransfection021001 nanoscience & nanotechnologyvaccinationSmall-angle neutron scatteringLipidslipid-polymer hybrid nanoparticlesQuaternary Ammonium Compounds030104 developmental biologychemistrylcsh:Biology (General)cationic polymerBiophysicsengineeringNanoparticlesRNAFemalePolymer blendBiopolymer0210 nano-technologyCovid-19
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Downregulation of thioredoxin-1-dependent CD95 S-nitrosation by Sorafenib reduces liver cancer

2020

Hepatocellular carcinoma (HCC) represents 80% of the primary hepatic neoplasms. It is the sixth most frequent neoplasm, the fourth cause of cancer-related death, and 7% of registered malignancies. Sorafenib is the first line molecular targeted therapy for patients in advanced stage of HCC. The present study shows that Sorafenib exerts free radical scavenging properties associated with the downregulation of nuclear factor E2-related factor 2 (Nrf2)-regulated thioredoxin 1 (Trx1) expression in liver cancer cells. The experimental downregulation and/or overexpression strategies showed that Trx1 induced activation of nitric oxide synthase (NOS) type 3 (NOS3) and S-nitrosation (SNO) of CD95 rece…

0301 basic medicine:Anatomy::Cells::Cells Cultured::Cell Line::Cell Line Tumor [Medical Subject Headings]Factor 2 relacionado con NF-E2Regulación hacia abajomedicine.medical_treatment[SDV]Life Sciences [q-bio]Clinical Biochemistry:Chemicals and Drugs::Amino Acids Peptides and Proteins::Proteins::Thioredoxins [Medical Subject Headings]ApoptosisBiochemistry:Phenomena and Processes::Chemical Phenomena::Biochemical Phenomena::Biochemical Processes::Nitrosation [Medical Subject Headings]Targeted therapyNeoplasias hepáticas:Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans [Medical Subject Headings]Mice0302 clinical medicineThioredoxins:Organisms::Eukaryota::Animals [Medical Subject Headings]lcsh:QH301-705.5Cell proliferationlcsh:R5-920GSNORChemistry:Diseases::Neoplasms::Neoplasms by Site::Digestive System Neoplasms::Liver Neoplasms [Medical Subject Headings]Liver NeoplasmsSorafenibFas receptor3. Good healthHepatocellular carcinomaCD95Liver cancerlcsh:Medicine (General)NOS3Liver cancerCarcinoma hepatocelularResearch Papermedicine.drugSorafenibHepatocarcinomaProliferación celularCarcinoma HepatocellularNitrosationDown-RegulationMice Nude[SDV.CAN]Life Sciences [q-bio]/CancerAntineoplastic AgentsNrf203 medical and health sciencesDownregulation and upregulationCell Line TumormedicineAnimalsHumansS-NitrosoglutatiónTiorredoxinas:Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell Growth Processes::Cell Proliferation [Medical Subject Headings]:Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Rodentia::Muridae::Murinae::Mice [Medical Subject Headings]:Diseases::Neoplasms::Neoplasms by Site::Digestive System Neoplasms::Liver Neoplasms::Carcinoma Hepatocellular [Medical Subject Headings]:Phenomena and Processes::Chemical Phenomena::Biochemical Phenomena::Biochemical Processes::Down-Regulation [Medical Subject Headings]Cell growthPhenylurea CompoundsOrganic Chemistry:Chemicals and Drugs::Chemical Actions and Uses::Pharmacologic Actions::Therapeutic Uses::Antineoplastic Agents [Medical Subject Headings][SDV.MHEP.HEG]Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology:Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Rodentia::Muridae::Murinae::Mice::Mice Mutant Strains::Mice Nude [Medical Subject Headings]medicine.diseasedigestive system diseases030104 developmental biologylcsh:Biology (General)ApoptosisDownregulation:Chemicals and Drugs::Organic Chemicals::Hydrocarbons::Hydrocarbons Cyclic::Hydrocarbons Aromatic::Benzene Derivatives::Phenylurea Compounds [Medical Subject Headings][SDV.SP.PHARMA]Life Sciences [q-bio]/Pharmaceutical sciences/PharmacologyCancer researchÓxido nítrico sintasa de tipo III030217 neurology & neurosurgery
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Melatonin Targets Metabolism in Head and Neck Cancer Cells by Regulating Mitochondrial Structure and Function.

2021

This study was funded by grants from the Ministerio de Economia, Industria y Competitividad y por el Fondo de Desarrollo Regional FEDER, Spain nº SAF2013-49019, SAF2017-85903-P, and from the Consejería de Innovación, Ciencia y Empresa, Junta de Andalucía (P07- CTS- 03135, P10- CTS- 5784, and CTS- 101), Spain. J.F. and L.M. have FPU fellowships from the Ministerio de Educación Cultura y Deporte, Spain. C.R.S. was a schorlarship holder from the Plan Propio de Investigación of the University of Granada.

0301 basic medicine:Phenomena and Processes::Chemical Phenomena::Biochemical Phenomena::Biochemical Processes::Phosphorylation::Oxidative Phosphorylation [Medical Subject Headings]PhysiologyClinical BiochemistrymelatoninMitochondrionBiochemistryMelatonina:Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans [Medical Subject Headings]0302 clinical medicine:Anatomy::Cells::Cells Cultured::Cell Line [Medical Subject Headings]head and neck cancer cells:Phenomena and Processes::Physiological Phenomena::Pharmacological Phenomena::Drug Resistance::Drug Resistance Neoplasm [Medical Subject Headings]MitophagyMitocondriasChemistryapoptosisglycolysisOXPHOSmitochondria030220 oncology & carcinogenesishormones hormone substitutes and hormone antagonistsmedicine.drug:Phenomena and Processes::Chemical Phenomena::Biochemical Phenomena::Biochemical Processes::Carbohydrate Metabolism::Glycolysis [Medical Subject Headings]Neoplasias de cabeza y cuello:Diseases::Neoplasms::Neoplasms by Site::Head and Neck Neoplasms [Medical Subject Headings]:Chemicals and Drugs::Inorganic Chemicals::Free Radicals::Reactive Oxygen Species [Medical Subject Headings]Mitofagiafree radicalsOxidative phosphorylationArticleMelatonin03 medical and health sciencesmedicine:Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell Growth Processes::Cell Proliferation [Medical Subject Headings]Molecular BiologyRadicales libresCell growth:Chemicals and Drugs::Amino Acids Peptides and Proteins::Proteins::DNA-Binding Proteins::Receptors Cytoplasmic and Nuclear::Receptors Melatonin [Medical Subject Headings]:Chemicals and Drugs::Chemical Actions and Uses::Pharmacologic Actions::Therapeutic Uses::Antineoplastic Agents [Medical Subject Headings]lcsh:RM1-950:Anatomy::Cells::Cellular Structures::Subcellular Fractions::Mitochondria [Medical Subject Headings]Cell Biologymedicine.diseaseHead and neck squamous-cell carcinoma:Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell Death::Apoptosis [Medical Subject Headings]Glucólisis030104 developmental biologylcsh:Therapeutics. PharmacologymitophagyApoptosisCancer cellCancer research:Chemicals and Drugs::Hormones Hormone Substitutes and Hormone Antagonists::Hormones::Melatonin [Medical Subject Headings]
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