Search results for "Reaction"

Indoor air pollutants stimulate interleukin-8-specific mRNA expression and protein secretion of alveolar macrophages.

1998

Indoor air pollutants may cause inflammatory changes of the airways and adjacent pulmonary tissue. After phagocytosis of inhaled particles alveolar macrophages (AM) release chemotactic mediators capable of attracting inflammatory cells into the lung tissue. To evaluate these mechanisms further peripheral blood mononuclear cells (PBMNC) and human AM (freshly recovered from the lower respiratory tract) were exposed to the indoor particles Soot FR 101 and Printex 90, the asbestos fiber Chrysotile B, and titanium dioxide (TiO2) at concentrations of 10 or 50 microg/10(6) cells for up to 8 h. The migration of granulocytes into the conditioned supernatants of AM and PBMNC was quantified by chemota…

Oral N-acetylcysteine attenuates the rat pulmonary inflammatory response to antigen.

2003

Oxidative stress is involved in the pathophysiology of inflammatory airway diseases including asthma; therefore, antioxidants might be of clinical benefit in asthma treatment. In the present study, the effects of N-acetylcysteine on sensitised brown Norway rats were examined. N-Acetylcysteine (3 mmol kg body weight(-1) administered orally) was given daily for 1 week before challenge and various antigen-induced pulmonary responses were studied. Antigen exposure increased lipid peroxidation in bronchoalveolar lavage fluid (BALF) and oxidised glutathione levels in lung tissue 2 h after challenge. Lung nuclear transcription factor-KB-binding activity was increased 2 h after challenge, and BALF …

Chronological expression of Ciliated Bronchial Epithelium 1 during pulmonary development

2009

Ciliated Bronchial Epithelium (CBE) 1 is a novel gene, which is expressed in ciliated cells. As cilia are important during embryogenesis, the present authors characterised the murine homologue of CBE1 (Cbe1) and compared its temporal expression during murine and human lung development. Cbe1 cDNA was cloned and characterised using sequencing, standard PCR and Western blotting. Mouse and human embryonic/fetal lungs (HELs) were harvested for mRNA analysis and protein localisation in vivo and in vitro using RT-PCR and immunohistochemistry. The Cbe1 amino acid sequence was >75% identical with CBE1 and its alternative splicing and tissue distribution were highly conserved. Pulmonary expression of…

GM-CSF expression by human lung microvascular endothelial cells: in vitro and in vivo findings.

2002

Recently, many findings indicate that granulocyte-macrophage colony-stimulating factor (GM-CSF) plays an important role in the pathogenesis of acute and chronic lung diseases. In the present paper, the production of this cytokine in human pulmonary microvascular endothelial cells (HPMEC) is investigated. In an in vitro study, quiescent HPMEC did not express GM-CSF, either at the transcriptional or at the protein level. After activation for 4 h with tumor necrosis factor (TNF)-α (30/300 U/ml), lipopolysaccharide (LPS; 0.1/1 μg/ml), or interleukin (IL)-1β (100 U/ml), a significant release of GM-CSF was measured by enzyme-linked immunosorbent assay, with a time-dependent increase over 72 h. IL…

Ist die Tuberkulinallergie Ausdruck einer Antigen-Antikörper-Reaktion?

1963

Tropomyosin: A panallergen that causes a worldwide allergic problem

2021

Background: Panallergens are proteins that take part in key processes of organisms and, therefore, are ubiquitously distributed with highly conserved sequences and structures. One class of these panallergens is composed of the tropomyosins. The highly heat-stable tropomyosins comprise the major allergens in crustaceans and mollusks, which make them important food allergens in exposed populations. Tropomyosins are responsible for a widespread immunoglobulin E cross-reactivity among allergens from different sources. Allergic tropomyosins are expressed in many species, including parasites and insects. Methods: This panallergen class is divided, according to it capacity of induced allergic symp…

Synthesis of chlorinated alkylbibenzyls and 9-chlororetene

1995

Abstract The synthesis of chlorinated alkylbibenzyls and 9-chlororetene is described. These compounds were synthesized for use as model compounds in analytical and toxicological characterization. The structures of the synthesized model compounds found to be very similar to those identified in pulp mill effluent and products. The 1H NMR and mass spectral data for the compounds are also reported.

Parameter dependence for the positive solutions of nonlinear, nonhomogeneous Robin problems

2020

We consider a parametric nonlinear Robin problem driven by a nonlinear nonhomogeneous differential operator plus an indefinite potential. The reaction term is $$(p-1)$$-superlinear but need not satisfy the usual Ambrosetti–Rabinowitz condition. We look for positive solutions and prove a bifurcation-type result for the set of positive solutions as the parameter $$\lambda >0$$ varies. Also we prove the existence of a minimal positive solution $$u_\lambda ^*$$ and determine the monotonicity and continuity properties of the map $$\lambda \rightarrow u_\lambda ^*$$.

Solutions and positive solutions for superlinear Robin problems

2019

We consider nonlinear, nonhomogeneous Robin problems with a (p − 1)-superlinear reaction term, which need not satisfy the Ambrosetti-Rabinowitz condition. We look for positive solutions and prove existence and multiplicity theorems. For the particular case of the p-Laplacian, we prove existence results under a different geometry near the origin.We consider nonlinear, nonhomogeneous Robin problems with a (p − 1)-superlinear reaction term, which need not satisfy the Ambrosetti-Rabinowitz condition. We look for positive solutions and prove existence and multiplicity theorems. For the particular case of the p-Laplacian, we prove existence results under a different geometry near the origin.

A multiplicity theorem for parametric superlinear (p,q)-equations

2020

We consider a parametric nonlinear Robin problem driven by the sum of a \(p\)-Laplacian and of a \(q\)-Laplacian (\((p,q)\)-equation). The reaction term is \((p-1)\)-superlinear but need not satisfy the Ambrosetti-Rabinowitz condition. Using variational tools, together with truncation and comparison techniques and critical groups, we show that for all small values of the parameter, the problem has at least five nontrivial smooth solutions, all with sign information.