Search results for "Reaction"
showing 10 items of 6134 documents
Triazolopyridines 23.1 Synthesis of 5,5'-bi[1,2,3]triazolo[5,1-a]isoquinoline
2004
5,5'-Bi[1,2,3]triazolo[5,1-a]isoquinoline 6 has been synthesised from [1,2,3]triazolo[5,1-a] isoquinoline 5 by four procedures, dimerisation by LDA, Stille and Suzuki self-coupling reactions, and Suzuki cross-coupling reaction. The last is the best. Compound 6 gives 1,1'- bi(acetoxymethyl)-3,3'-biisoquinoline 10 by triazolo ring opening by acetic acid. Abarca Gonzalez, Belen, Belen.Abarca@uv.es ; Ballesteros Campos, Rafael, Rafael.Ballesteros@uv.es ; Gay Roig, Bernat, bergay@alumni.uv.es
NURE: An ERC project to study nuclear reactions for neutrinoless double beta decay
2017
Neutrinoless double beta decay (0{\nu}\b{eta}\b{eta}) is considered the best potential resource to determine the absolute neutrino mass scale. Moreover, if observed, it will signal that the total lepton number is not conserved and neutrinos are their own anti-particles. Presently, this physics case is one of the most important research beyond Standard Model and might guide the way towards a Grand Unified Theory of fundamental interactions. Since the \b{eta}\b{eta} decay process involves nuclei, its analysis necessarily implies nuclear structure issues. The 0{\nu}\b{eta}\b{eta} decay rate can be expressed as a product of independent factors: the phase-space factors, the nuclear matrix elemen…
Value of the Axial-Vector Coupling Strength in β and ββ Decays : A Review
2017
In this review the quenching of the weak axial-vector coupling constant, $g_{\rm A}$, is discussed in nuclear $\beta$ and double-$\beta$ decays. On one hand, the nuclear-medium and nuclear many-body effects are separated, and on the other hand the quenching is discussed from the points of view of different many-body methods and different $\beta$-decay and double-$\beta$-decay processes. Both the historical background and the present status are reviewed and contrasted against each other. The theoretical considerations are tied to performed and planned measurements, and possible new measurements are urged, whenever relevant and doable. Relation of the quenching problem to the measurements of …
Rejuvenative Efficacy and NF-κB Pathway-Dependent Mechanism of Human Platelet Lysate on Skin Aging
2021
Background: Skin aging is an irreversible process associated with poor self-regenerative capability. Human platelet lysate (hPL) contains rich amounts of growth factors, benefiting tissue regeneration. To explore the anti-aging efficacy and mechanism of hPL on skin, this study employed nude mice and human dermal fibroblasts (HDFs) to perform in vitro and in vivo assays. Methods: Flow cytometry and ELISA were conducted for quality control of hPL. In vivo, skin texture grading, histopathological observation, immunofluorescence analysis, and SOD and MDA assays were conducted to determine the effects of hPL on epidermis and dermis of aged skin. In vitro, senescence-associated-beta-galactosidase…
Age-associated telomere shortening in mouse oocytes
2013
Abstract Background Oocytes may undergo two types of aging. The first is induced by exposure to an aged ovarian microenvironment before being ovulated, known as ‘reproductive or maternal aging’, and the second by either a prolonged stay in the oviduct before fertilization or in vitro aging prior to insemination, known as ‘postovulatory aging’. However, the molecular mechanisms underlying these aging processes remain to be elucidated. As telomere shortening in cultured somatic cells triggers replicative senescence, telomere shortening in oocytes during reproductive and postovulatory aging may predict developmental competence. This study aimed to ascertain the mechanisms underlying altered te…
Yeast vectors for the integration/expression of any sequence at theTYR1 locus
2007
We have constructed new yeast vectors for targeted integration and conditional expression of any sequence at the Saccharomyces cerevisiae TYR1 locus which becomes disrupted. We show that vector integration is not neutral, causing prototrophy for tyrosine and auxotrophy for the vector's selectable marker (uracil or leucine, depending on the vector used). This feature allows a double screening of transformed yeast cells, improving the identification of colonies with the desired chromosomal structure. The GAL10 gene promoter has been added to drive conditional expression of cloned sequences. Using these vectors, chromosomal structure verification of recombinant clones is no longer necessary, s…
ABO genotyping by PCR-RFLP and cloning and sequencing
2005
A refined PCR-RFLP based method was established to genotype ABO blood groups. The main objective of this study was to make the techniques also suitable for working with degraded DNA. Specific primer design was carried out to choose fragments shorter than 200 bp as necessary in forensic and archaeological applications. Four fragments of exon 6 and 7 of the ABO gene were amplified and digested by in total 7 restriction endonucleases. Particular attention was paid to the base changes at nucleotide positions 261(delG), 297, 526, 703, 721, 771, 796 and 1060(delC) in order to distinguish the six common alleles A101, A201, B, O01, O02 and O03. Furthermore, this method also enables determination of…
Simultaneous detection of the seven main tomato-infecting RNA viruses by two multiplex reverse transcription polymerase chain reactions
2012
Cucumber mosaic virus, Tomato spotted wilt virus, Tomato mosaic virus, Tomato chlorosis virus, Pepino mosaic virus, Torrado tomato virus and Tomato infectious chlorosis virus cause serious damage and significant economic losses in tomato crops worldwide. The early detection of these pathogens is essential for preventing the viruses from spreading and improving their control. In this study, a procedure based on two multiplex RT-PCRs was developed for the sensitive and reliable detection of these seven viruses. Serial dilutions of positive controls were analysed by this methodology, and the results were compared with those obtained by ELISA and singleplex versions of RT-PCR. The multiplex and…
Effects of low-gamma tACS on primary motor cortex in implicit motor learning
2019
Abstract In the primary motor cortex (M1), rhythmic activity in the gamma frequency band has been found during movement planning, onset and execution. Although the role of high-gamma oscillatory activity in M1 is well established, the contribution of low-gamma activity is still unexplored. In this study, transcranial alternating current stimulation (tACS) was used with the aim to specifically modulate low-gamma frequency band in M1, during an implicit motor learning task. A 40 Hz-tACS was applied over the left M1 while participants performed a serial reaction time task (SRTT) using their right hand. The task required the repetitive execution of sequential movements in response to sequences …
Interference of left and right cerebellar rTMS with procedural learning.
2004
Abstract Increasing evidence suggests cerebellar involvement in procedural learning. To further analyze its role and to assess whether it has a lateralized influence, in the present study we used a repetitive transcranial magnetic stimulation interference approach in a group of normal subjects performing a serial reaction time task. We studied 36 normal volunteers: 13 subjects underwent repetitive transcranial magnetic stimulation on the left cerebellum and performed the task with the right (6 subjects) or left (7 subjects) hand; 10 subjects underwent repetitive transcranial magnetic stimulation on the right cerebellum and performed the task with the hand ipsilateral (5 subjects) or contral…