Search results for "Real-Time"
showing 10 items of 881 documents
A set of indices to assess the real performance of daylight-linked control systems
2017
Abstract The installation of Building Automation Control Systems (BACs) in general is an effective action to achieve relevant energy savings. Commercial BACs, installed in residential or small offices, often include functions of lighting control, acting as Daylight-Linked Control Systems (DLCSs). Nevertheless, because system performance is strictly dependent on different parameters, BAC’s hardware and software configuration and as well as an inaccurate commissioning do not always allow a perfect execution of the desired tasks; therefore, the system could not work as expected. Moreover, it is well known that energy saving potential is specifically related to the variability of the light envi…
Real-Time Polymerase Chain Reaction for Detection of Schistosoma DNA in Small-Volume Urine Samples Reflects Focal Distribution of Urogenital Schistos…
2014
Schistosoma haematobium eggs and Schistosoma DNA levels were measured in urine samples from 708 girls recruited from 18 randomly sampled primary schools in South Africa. Microscopic analysis of two 10-mL urine subsamples collected on three consecutive days confirmed high day-to-day variation; 103 (14.5%) girls had positive results at all six examinations, and at least one positive sample was seen in 225 (31.8%) girls. Schistosoma-specific DNA, which was measured in a 200-μL urine subsample by using real-time polymerase chain reaction, was detected in 180 (25.4%) cases, and levels of DNA corresponded significantly with average urine egg excretion. In concordance with microscopic results, pol…
Validation of a set of reference genes to study response to herbicide stress in grasses
2012
Abstract Background Non-target-site based resistance to herbicides is a major threat to the chemical control of agronomically noxious weeds. This adaptive trait is endowed by differences in the expression of a number of genes in plants that are resistant or sensitive to herbicides. Quantification of the expression of such genes requires normalising qPCR data using reference genes with stable expression in the system studied as internal standards. The aim of this study was to validate reference genes in Alopecurus myosuroides, a grass (Poaceae) weed of economic and agronomic importance with no genomic resources. Results The stability of 11 candidate reference genes was assessed in plants res…
Bypassing hazard of housekeeping genes: their evaluation in rat granule neurons treated with cerebrospinal fluid of multiple sclerosis subjects
2015
Gene expression studies employing real-time PCR has become an intrinsic part of biomedical research. Appropriate normalization of target gene transcript(s) based on stably expressed housekeeping genes is crucial in individual experimental conditions to obtain accurate results. In multiple sclerosis (MS), several gene expression studies have been undertaken, however, the suitability of housekeeping genes to express stably in this disease is not yet explored. Recent research suggests that their expression level may vary under different experimental conditions. Hence it is indispensible to evaluate their expression stability to accurately normalize target gene transcripts. The present study ai…
Wi-Dia: Data-Driven Wireless Diagnostic Using Context Recognition
2018
The recent densification of Wi-Fi networks is exacerbating the effects of well-known pathologies including hidden nodes and flow starvation. This paper provides an automatic diagnostic tool for detecting the source roots of performance impairments by recognizing the wireless operating context. Our tool for Wi-Fi diagnostic, named Wi-Dia, exploits machine learning methods and uses features related to network topology and channel utilization, without impact on regular network operations and working in real-time. Real-time per-link Wi-Fi diagnosis enables recovering actions for context-specific treatments. Wi-Dia classifier recognizes different classes of interference; it is jointly trained us…
Monitoring High-Quality Wine Production using Wireless Sensor Networks
2009
This work reports the experience on the design and deployment of a WSN-based system for monitoring the productive cycle of high-quality wine in a Sicilian winery. Besides providing the means for pervasive monitoring of the cultivated area, the project described here is aimed to support the producer in ensuring the overall quality of their production, in terms of accurate planning of interventions in the field, and preservation of the stored product. Wireless Sensor Networks are employed as the sensing infrastructure of a distributed system for the control of a prototypal productive chain; nodes have been deployed both in the field and in the cellar, where wine aging is performed, and data i…
Robust Energy Scheduling in Vehicle-to-Grid Networks
2017
The uncertainties brought by intermittent renewable generation and uncoordinated charging behaviors of EVs pose great challenges to the reliable operation of power systems, which motivates us to explore the integration of robust optimization with energy scheduling in V2G networks. In this article, we first introduce V2G robust energy scheduling problems and review the stateof- the art contributions from the perspectives of renewable energy integration, ancillary service provision, and proactive demand-side participation in the electricity market. Second, for each category of V2G applications, the corresponding problem formulations, robust solution concepts, and design approaches are describ…
A European multicentre evaluation of detection and typing methods for human enteroviruses and parechoviruses using RNA transcripts
2020
Polymerase chain reaction (PCR) detection has become the gold standard for diagnosis and typing of enterovirus (EV) and human parechovirus (HPeV) infections. Its effectiveness depends critically on using the appropriate sample types and high assay sensitivity as viral loads in cerebrospinal fluid samples from meningitis and sepsis clinical presentation can be extremely low. This study evaluated the sensitivity and specificity of currently used commercial and in‐house diagnostic and typing assays. Accurately quantified RNA transcript controls were distributed to 27 diagnostic and 12 reference laboratories in 17 European countries for blinded testing. Transcripts represented the four human EV…
RoadMic: Road Surface Monitoring Using Vehicular Sensor Networks with Microphones
2010
Road surface analysis including pothole reports is an important problem for road maintainers and drivers. In this paper we propose a methodology for pothole detection using mobile vehicles equipped with off the shelf microphone and global positioning devices attached to an on-board computer. The approach is generic enough to be extended for other kind of event detection using different sensors as well. The vehicles are driving on public streets and measuring pothole induced sound signals. Our approach was tested and evaluated by real world experiments in a road segment for which we had established the ground truth beforehand. The results show pothole detection with high accuracy despite the…
A bacterial metabolite, trimethylamine N-oxide, disrupts the hemostasis balance in human primary endothelial cells but no coagulopathy in mice
2019
: The gut microbial metabolite, trimethylamine N-oxide (TMAO), was previously reported to induce platelet hypersensitivity, which leads to thrombotic risk. However, the molecular mechanism underlying the effects of TMAO on endothelial cells (EC), which is the primary vessel wall contact with the lumen, remains unclear. Here, we investigated the impact of TMAO on procoagulant activity (PCA) in EC and mice, for a possible link between microbiota and coagulation. To test the PCA of TMAO in EC, we performed one-stage clotting assays and converted into PCA. Antitissue factor (TF) antibody was used to test the TF role in PCA. Quantitative PCR was performed to measure the TF, thrombomodulin, IL-6,…