Search results for "Real"
showing 10 items of 4449 documents
Surrealismo e dintorni
2010
Complexity of IL-1β induced gene expression pattern in human articular chondrocytes
1997
The mRNA fingerprinting technique, differential display reverse transcription polymerase chain (DDRT-PCR), was used to detect changes in the overall pattern of gene expression in human articular knee chondrocytes-induced by interleukin-1 beta (IL-1 beta), the prototypical inducer of catabolic responses in degenerate joint diseases. One hundred different primer combinations generated approximately 10,000 different PCR fragments for IL-1 beta treated, as well as for untreated human chondrocytes, cultivated in alginate beads. This represented 53% of all expressed chondrocyte genes as based on statistical considerations. Side by side comparisons of differential display patterns originating from…
La comitiva de Alfonso XI: notas para su estudio
1980
Wykorzystanie modelu PESO do pomiaru poziomu ekspozycji i zaangażowania w prowadzonych w mediach społecznościowych działaniach promocyjnych pośrednik…
2019
The growing popularity of social media is accompanied by an increase in the importance of the quality and frequency of content published in the Internet. This entails constant analysis, among others, in the field of exposure and the involvement of Internet users in the media. Among the many available tools to carry out the above analysis, in this work the PESO model – Don Bartholomew was used. The aim of the paper is to implement the PESO model (measuring social media on many levels) on the real estate market and to analyze the level of exposure and social media involvement by real estate agents. The detailed objective is to analyze the information content of social media publications, vide…
IL CASTELLACCIO DI MONTE CAPUTO A MONREALE: TRE PIANTE A CONFRONTO
2021
This contribution describes and compares three plans of Castellaccio respectively from 1739, from end-18th and end-19th century. The comparison shows the difference between the ancient surveys, which aim to provide an ideal image, and the modern ones, which document reality and represent it scientifically.
AD ERUDIENDAM FIDELIUM PLEBEM ESEGESI DEI PRIMI SUNTI SCRITTURALI PALEOSLAVI (ss. IX-XI)
2010
Transcriptome-wide identification of transient RNA G-quadruplexes in human cells
2018
Guanine-rich RNA sequences can fold into four-stranded structures, termed G-quadruplexes (G4-RNAs), whose biological roles are poorly understood, and in vivo existence is debated. To profile biologically relevant G4-RNA in the human transcriptome, we report here on G4RP-seq, which combines G4-RNA-specific precipitation (G4RP) with sequencing. This protocol comprises a chemical crosslinking step, followed by affinity capture with the G4-specific small-molecule ligand/probe BioTASQ, and target identification by sequencing, allowing for capturing global snapshots of transiently folded G4-RNAs. We detect widespread G4-RNA targets within the transcriptome, indicative of transient G4 formation in…
An unconventional role for miRNA: let-7 activates Toll-like receptor 7 and causes neurodegeneration
2011
Activation of innate immune receptors by host-derived factors exacerbates CNS damage, but the identity of these factors remains elusive. We uncovered an unconventional role for the microRNA let-7, a highly abundant regulator of gene expression in the CNS, in which extracellular let-7 activates the RNA-sensing Toll-like receptor (TLR) 7 and induces neurodegeneration through neuronal TLR7. Cerebrospinal fluid (CSF) from individuals with Alzheimer’s disease contains increased amounts of let-7b, and extracellular introduction of let-7b into the CSF of wild-type mice by intrathecal injection resulted in neurodegeneration. Mice lacking TLR7 were resistant to this neurodegenerative effect, but thi…
Endothelial transcriptomic changes induced by oxidized low density lipoprotein disclose an up-regulation of Jak-Stat pathway.
2015
Oxidized low density lipoproteins (oxLDLs) act as an etiological factor in the development of atherosclerosis by modifying the biological properties of endothelial cells through mechanisms of vascular inflammation. To deepen the oxLDL changes at cellular level, a transcriptomic analysis of human umbilical artery endothelial cells (HUAECs) treated with oxLDL was performed to identify the modified signaling pathways. Total RNA was isolated from HUAECs treated with oxLDL (100 μg/ml). Gene expression analysis was carried out using Affymetrix oligonucleotide microarrays. Biological pathway analysis was performed using Ingenuity Pathway Analysis software. Microarray assay demonstrated that oxLDL …
Endothelial Progenitor Cells: A Real Hope or an Unrealizable Dream? Which Measures or Strategies Are Necessary for making EPCs a clinical reality? Fo…
2017
It is the time to re-examine with new eyes the findings obtained to date on endothelial progenitor cells (EPCs), in order to eliminate the limitations and to shed light on such findings. This revision might be of help for reordering them and consequently to create a clear puzzle. This last might provide evidence relating to important aspects, including defining, isolating and characterizing EPCs by establishing standardized criteria for EPC research; discriminating between appropriate sub-populations for cell therapy; timing; dosing; priming of cells; and the delivery mode for different applications. Furthermore, influencing factors might also be identified, with the aim of focusing resourc…