Search results for "Red"

showing 10 items of 23890 documents

Recombinant laccase from Pediococcus acidilactici CECT 5930 with ability to degrade tyramine

2017

Biogenic amines degradation by bacterial laccases is little known, so we have cloned and heterologously expressed, in E. coli, a new laccase from Pediococcus acidilactici CECT 5930 (Lpa5930), a lactic acid bacterium commonly found in foods able to degrade tyramine. The recombinant enzyme has been characterized by physical and biochemical assays. Here we report the optimization of expression and purification procedures of this laccase. DNA encoding sequence of laccase from P. acidilactici was amplified by PCR and cloned into the expression plasmid pET28a for induction by isopropyl-β-D-thiogalactoipyranoside. Protein expression was performed in E. coli BL21(DE3) harboring pGro7 plasmid expres…

0106 biological sciences0301 basic medicineArabinoseMolecular biologylcsh:MedicineLaccasesBiochemistryBiotecnologia01 natural sciencesSubstrate Specificitylaw.inventionDatabase and Informatics Methodschemistry.chemical_compoundlawRecombinant Protein PurificationCloning MolecularAmineslcsh:Sciencechemistry.chemical_classificationMultidisciplinaryABTSbiologyOrganic CompoundsTemperatureHydrogen-Ion ConcentrationTyramineRecombinant ProteinsEnzymesChemistryRecombination-Based AssayBiochemistryPhysical SciencesRecombinant DNAElectrophoresis Polyacrylamide GelOxidation-ReductionSequence AnalysisResearch ArticleProtein PurificationBioinformaticsTyramineLibrary ScreeningDNA constructionResearch and Analysis Methods03 medical and health sciencesBacterial ProteinsSequence Motif Analysis010608 biotechnologyAmino Acid SequenceBenzothiazolesPediococcus acidilacticiLaccaseMolecular Biology Assays and Analysis TechniquesBase SequenceMolecular massLaccaseOrganic Chemistrylcsh:RChemical CompoundsBiology and Life SciencesProteinsPediococcus acidilacticiSequence Analysis DNAbiology.organism_classificationMolecular biology techniques030104 developmental biologyEnzymechemistryPlasmid ConstructionEnzymologySpectrophotometry Ultravioletlcsh:QSulfonic AcidsEnzimsProteïnesPurification TechniquesPLOS ONE
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Preliminary Study on the In vitro and In vivo Effects of Asparagopsis taxiformis Bioactive Phycoderivates on Teleosts

2016

Several compounds from marine organisms have been studied for their potential use in aquaculture. Among the red algae, Asparagopsis taxiformis is considered one of the most promising species for the production of bioactive metabolites with numerous proposed applications. Here, the in vitro antibacterial activity, the easy handling and the absence of adverse effects on marine fish species are reported. Depending on the seasonal period of sampling, ethanol extracts of A. taxiformis exhibited significantly different inhibitory activity against fish pathogenic bacteria. The extract obtained in late spring showed strong antibacterial activity against Aeromonas salmonicida subsp. salmonicida, Vib…

0106 biological sciences0301 basic medicineAsparagopsis taxiformisaquaculture.Physiologyhematological parametersRed algaemedicine.disease_cause01 natural scienceslcsh:PhysiologyMicrobiologyAsparagopsis taxiformis Dicentrarchus labrax Sparus aurataantibacterial activity hematological parameter oxidative stresstoxicity03 medical and health sciencesAquacultureantibacterial activitySparus aurataPhysiology (medical)medicineoxidative stressDicentrarchus labraxSea basshematological parameterOriginal ResearchVibrio alginolyticusImmune stimulationlcsh:QP1-981biologybusiness.industry010604 marine biology & hydrobiologytoxicityPathogenic bacteriabiology.organism_classificationAeromonas salmonicida030104 developmental biologyPhotobacterium damselaeAsparagopsis taxiformisbusinessFrontiers in Physiology
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Thalassocella blandensis gen. nov., sp. nov., a novel member of the family Cellvibrionaceae

2020

9 pages, 1 figure, 3 tables

0106 biological sciences0301 basic medicineBiology010603 evolutionary biology01 natural sciencesMicrobiology03 medical and health sciencesSaccharophagus degradansGammaproteobacteriaMagnesium ionGenome sizeEcology Evolution Behavior and SystematicsStrain (chemistry)Marine bacteriaAgarilyticaGeneral MedicineAA16S ribosomal RNAbiology.organism_classificationHalophileThalassocella030104 developmental biologyBiochemistryCellvibrionaceaeANITeredinibacterEnergy sourceInternational Journal of Systematic and Evolutionary Microbiology
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New national and regional bryophyte records, 55

2018

1. Acroschisma wilsonii (Hook.f.) A.JaegerContributor: H. Bednarek-OchyraVenezuela: (1) Merida, Paramo de Mucubaji, vertiente de la Laguna de los Patos, Sierra de Santo Domingo, sobre roca ignea, 3...

0106 biological sciences0301 basic medicineBryofloraForestryBryologyPlant ScienceBryology030108 mycology & parasitology15. Life on land01 natural sciencesEcology Evolution Behavior and Systematic03 medical and health sciencesPlant scienceGeographyBryology Bryoflora Floristic recordIUCN Red ListBryophyte[SDE.BE]Environmental Sciences/Biodiversity and EcologySouthern HemisphereEcology Evolution Behavior and SystematicsFloristic recordComputingMilieux_MISCELLANEOUS010606 plant biology & botany
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In vivo photoprotection mechanisms observed from leaf spectral absorbance changes showing VIS–NIR slow-induced conformational pigment bed changes

2019

Abstract Regulated heat dissipation under excessive light comprises a complexity of mechanisms, whereby the supramolecular light-harvesting pigment–protein complex (LHC) shifts state from light harvesting towards heat dissipation, quenching the excess of photo-induced excitation energy in a non-photochemical way. Based on whole-leaf spectroscopy measuring upward and downward spectral radiance fluxes, we studied spectrally contiguous (hyperspectral) transient time series of absorbance A(λ,t) and passively induced chlorophyll fluorescence F(λ,t) dynamics of intact leaves in the visible and near-infrared wavelengths (VIS–NIR, 400–800 nm) after sudden strong natural-like illumination exposure. …

0106 biological sciences0301 basic medicineChlorophyllMaterials sciencePassive chlorophyll a fluorescencePigment–protein dynamicsLightHyperspectral remote sensingAnalytical chemistryJuglansPlant Science01 natural sciencesBiochemistryEnergy quenchingFluorescenceAbsorbance03 medical and health sciencesTransmittanceFiber Optic TechnologySpectroscopyChlorophyll fluorescencechemistry.chemical_classificationSpectroscopy Near-InfraredAbsorbed photosynthetic active radiation (APAR)Non-photochemical quenching (NPQ)Cell BiologyGeneral MedicineEquipment DesignPigments BiologicalPhotochemical ProcessesCarotenoidsPlant LeavesWavelength030104 developmental biologychemistryXanthophyllRadianceOriginal ArticleAbsorbance shiftMorusControlled heat dissipation010606 plant biology & botanyPhotosynthesis Research
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How to fight multiple enemies : target-specific chemical defences in an aposematic moth

2017

Animals have evolved different defensive strategies to survive predation, among which chemical defences are particularly widespread and diverse. Here we investigate the function of chemical defence diversity, hypothesizing that such diversity has evolved as a response to multiple enemies. The aposematic wood tiger moth (Arctia plantaginis) displays conspicuous hindwing coloration and secretes distinct defensive fluids from its thoracic glands and abdomen. We presented the two defensive fluids from laboratory-reared moths to two biologically relevant predators, birds and ants, and measured their reaction in controlled bioassays (no information on colour was provided). We found that defensive…

0106 biological sciences0301 basic medicineColorAposematismBiologyMoths010603 evolutionary biology01 natural sciencesGeneral Biochemistry Genetics and Molecular BiologyPredationBirds03 medical and health sciencesSpecies SpecificityAnimalsaposematismta116General Environmental ScienceGeneral Immunology and MicrobiologyEcologyEcologyAntsfungipredator–prey interactionschemical defencesGeneral MedicinepyrazinesBiological EvolutionBody Fluids030104 developmental biologyPredatory Behaviorta1181General Agricultural and Biological SciencesProceedings of the Royal Society B : Biological Sciences
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Conservation of the endangered Mediterranean tortoise Testudo hermanni hermanni: The contribution of population genetics and historical demography

2016

International audience; Estimating the genetic variation and demographic trends of species in decline is of major concern in conservation genetics. This study contributes to understanding how historical and anthropogenic factors shape the distribution of current genetic diversity in one of the most endangered reptiles in Western Europe, the Hermann's tortoise Testudo hermanni hermanni. We used 17 microsatellite loci, chosen from a pyrosequencing library specifically developed for the subspecies to genotype eight populations distributed over about 30 sample localities covering almost the entire geographic distribution of the sub-species. The population genetic results reflect a very strong g…

0106 biological sciences0301 basic medicineConservation geneticsDemographic historyTortoiseDemographic history[SDV]Life Sciences [q-bio]PopulationEndangered speciesSettore BIO/05 - ZoologiaZoologyBiology010603 evolutionary biology01 natural sciencesTestudo hermanni03 medical and health sciences14. Life underwatereducationMicrosatellitesConservation geneticEcology Evolution Behavior and SystematicsNature and Landscape ConservationConservation genetics; Demographic history; Microsatellites; Testudo hermanni Genetic structure; Conservation issueseducation.field_of_studyTestudo hermanniEcologyConservation issuesAmbientaleMicrosatellite15. Life on landbiology.organism_classificationTestudo hermanni Genetic structureConservation genetics Demographic history Microsatellites Testudo hermanni Genetic structure Conservation issues030104 developmental biologyThreatened speciesGenetic structureGenetic structureConservation genetics
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Northern European Salmo trutta (L.) populations are genetically divergent across geographical regions and environmental gradients

2020

The salmonid fish Brown trout is iconic as a model for the application of conservation genetics to understand and manage local interspecific variation. However, there is still scant information about relationships between local and large-scale population structure, and to what extent geographical and environmental variables are associated with barriers to gene flow. We used information from 3,782 mapped SNPs developed for the present study and conducted outlier tests and gene–environment association (GEA) analyses in order to examine drivers of population structure. Analyses comprised >2,600 fish from 72 riverine populations spanning a central part of the species' distribution in norther…

0106 biological sciences0301 basic medicineConservation geneticsSELECTIONPopulationsalmonidCONSERVATIONlcsh:Evolutiongenotype‐environment association010603 evolutionary biology01 natural sciencesGene flow03 medical and health sciencesbrown troutLOCAL ADAPTATIONSampling designlcsh:QH359-425GeneticsGENOME SCANS14. Life underwaterSalmoeducationEcology Evolution Behavior and SystematicsLocal adaptationGenotype‐environment associationeducation.field_of_studyCLIMATE-CHANGEbiologyBROWN TROUTSTRUCTURED POPULATIONSR-PACKAGESampling (statistics)genotype-environment associationVDP::Matematikk og Naturvitenskap: 400biology.organism_classification030104 developmental biologyEvolutionary biologyoutlier testTEMPORAL-CHANGESOutlierGeneral Agricultural and Biological SciencesASCERTAINMENT BIASlocal adaptation
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Spatio-temporal dynamics of genetic variation in the Iberian lynx along its path to extinction reconstructed with ancient DNA

2017

here is the tendency to assume that endangered species have been both genetically and demographically healthier in the past, so that any genetic erosion observed today was caused by their recent decline. The Iberian lynx (Lynx pardinus) suffered a dramatic and continuous decline during the 20th century, and now shows extremely low genome- and species-wide genetic diversity among other signs of genomic erosion. We analyze ancient (N = 10), historical (N = 245), and contemporary (N = 172) samples with microsatellite and mitogenome data to reconstruct the species' demography and investigate patterns of genetic variation across space and time. Iberian lynx populations transitioned from low but …

0106 biological sciences0301 basic medicineConservation of Natural ResourcesMetapopulationBiologyLincesExtinction Biological010603 evolutionary biology01 natural sciencesEndangered speciesgenetic erosion03 medical and health sciencesGenetic driftGenetic variationGeneticsAnimalsDNA AncientGenetic erosionancient DNAMolecular BiologyQH426Institut für Biochemie und BiologieDiscoveriesEcology Evolution Behavior and SystematicsPaleobiologíaGenetic diversityQLGenomeExtinctionAncient DNAEcologyQHEndangered SpeciesGenetic DriftGenetic VariationPaleogeneticsParque nacional de DoñanaSequence Analysis DNAIberian lynxGenéticahumanities030104 developmental biologyAncient DNAGenome MitochondrialLynxPaleogeneticsGenetic erosionpaleogeneticsMicrosatellite Repeats
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Throwing down a genomic gauntlet on fisheries-induced evolution

2021

Beginning with studies on crypsis and camouflage, the hypothesis that predators can generate evolutionary change in their prey has a long and rich history (1). Few predators, however, rival humans in their potential to generate selection responses and concomitant phenotypic change on contemporary timescales. In the 1930s, J. B. S. Haldane (2) mused that fishing would be an ideal candidate for such “observable evolution” within a human lifetime, proceeding “with extreme and abnormal speed.” However, it was not until the late 1970s that research on fisheries-induced evolution (FIE) gained a substantive scientific foothold, beginning with thought-provoking work on Canadian whitefish ( Coregonu…

0106 biological sciences0301 basic medicineCoregonus clupeaformisFishingFisheriesevoluutioBiodiversity437430Polymorphism Single Nucleotide010603 evolutionary biology01 natural sciencesPredation03 medical and health sciencesPer capitaAnimals14. Life underwaterSemelparity and iteroparityPopulation DensityMultidisciplinaryPopulation BiologybiologykalakannatFishesGenomicsgenomiikkaBiological Sciencesbiology.organism_classificationBiological EvolutionkalastusFisherykalatalousOverexploitation030104 developmental biologyCrypsisCommentaryProceedings of the National Academy of Sciences
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