Search results for "Regulator"

L'attività normativa del governo nel periodo 2017-2019

2022

L’articolo descrive l’attività normativa del governo nel periodo 2017-2019. Per quanto riguarda le procedure di programmazione e revisione della spesa pubblica, le riforme del 2016 finalizzate alla riduzione della spesa dei Ministeri hanno trovato applicazione solo nel 2017, mentre nel 2018 e nel 2019 ulteriori risparmi sono stati conseguiti mediante apposite previsioni delle leggi di bilancio. Le principali innovazioni in materia di liberalizzazione sono state portate a termine nel 2017, mentre non vi sono stati interventi rilevanti nel biennio successivo. Per quanto concerne il decentramento di funzioni amministrative, non si sono riscontrati progressi. Le politiche di privatizzazione son…

Novel Insights into the Cellular Localization and Regulation of the Autophagosomal Proteins LC3A, LC3B and LC3C

2020

Macroautophagy is a conserved degradative process for maintaining cellular homeostasis and plays a key role in aging and various human disorders. The microtubule-associated protein 1A/1B light chain 3B (MAP1LC3B or LC3B) is commonly analyzed as a key marker for autophagosomes and as a proxy for autophagic flux. Three paralogues of the LC3 gene exist in humans: LC3A, LC3B and LC3C. The molecular function, regulation and cellular localization of LC3A and LC3C have not been investigated frequently, even if a similar function to that described for LC3B appears likely. Here, we have selectively decapacitated LC3B by three separate strategies in primary human fibroblasts and analyzed the evoked e…

Non-cell autonomous and non-catalytic activities of ATX in the developing brain

2015

The intricate formation of the cerebral cortex requires a well-coordinated series of events, which are regulated at the level of cell-autonomous and non-cell autonomous mechanisms. Whereas cell-autonomous mechanisms that regulate cortical development are well-studied, the non cell-autonomous mechanisms remain poorly understood. A non-biased screen allowed us to identify Autotaxin (ATX) as a non cell-autonomous regulator of neural stem cell proliferation. ATX (also known as ENPP2) is best known to catalyze lysophosphatidic acid (LPA) production. Our results demonstrate that ATX affects the localization and adhesion of neuronal progenitors in a cell autonomous and non-cell autonomous manner, …

Micropropagazione di Platycodon grandiflorus

2009

Micropropagation of Genista aetnensis [(Raf. ex Biv.)DC]

2015

Genista aetnensis [(Raf. ex Biv.)DC] is a large deciduous shrub or small tree native to the Italian islands of Sardinia and Sicily. Being winter hardy and characterized by high plasticity in altitude and ecology, the species is grown in gardens and landscaping, both for flower and for its attractive shape. Genista species are generally propagate by seed or semi hardwood cuttings. In this report an efficient in vitro technique for propagation of G. aetnensis was investigated. Multiple shoots were induced on nodal segments of a mature plant of Genista aetnensis . The Murashige and Skoog medium, augmented with different concentrations of benzyladenine either singly or in combination with indol…

A long non-coding RNA controls parasite differentiation in African trypanosomes

2020

Trypanosoma bruceicauses African sleeping sickness, a fatal human disease. Its differentiation from replicative slender form into quiescent stumpy form promotes host survival and parasite transmission. Long noncoding RNAs (lncRNAs) are known to regulate cell differentiation. To determine whether lncRNAs are involved in parasite differentiation we used RNAseq to survey theT. bruceilncRNA gene repertoire, identifying 1,428 previously uncharacterized lncRNA genes. We analysedgrumpy, a lncRNA located immediately upstream of an RNA-binding protein that is akeydifferentiation regulator. Grumpy over-expression resulted in premature parasite differentiation into the quiescent stumpy form, and subse…

Antigen dose-dependent suppression of murine IgE responses is mediated by CD4−CD8− double-negative T cells

2010

Background The IgE response against protein antigens is profoundly influenced by the dose used for sensitization. Objective The aim of the study was to identify immune cells that are involved in antigen dose-dependent regulation of IgE formation. Methods Wild-type mice as well as T helper (Th)1-deficient IL-12p40(-/-) and IFN-gamma(-/-) mice were immunized by repeated intraperitoneal injection of either low doses (K01 mice) or high doses (K100 mice) of keyhole limpet haemocyanin adsorbed to aluminium hydroxide. Splenocytes of immunized mice were restimulated in vitro and antigen-dependent T cell proliferation and cytokine production were measured. The frequency of regulatory T cell subsets …

Investigation of the resistance of different varieties of buckwheat to infectious diseases after the pre-sowing treatment of seeds and vegetating pla…

2016

In the work it was investigated the influence of sodium humate, biological products "Vermisol", "Vitasym" and "Bioeco-funge-1" on buckwheat lesion of Botrytis bunch rot, Downy mildew, Ascochyta blight, bacteriosis, burn virus and a complex of diseases after pre-sowing treatment of seeds and vegetating plants of Victoria, Roxolana, Kara-Dag, Rubra, Zelenokvitkova-90,Stepova, Elena, Aelita, Lada and Fagopyrum tataricum Gaertn buckwheat varieties. The prospects of these biological products use under agrocenosis conditions to protect the crops of buckwheat from the complex of diseases were shown. The highest effi- ciency identified for the drug "Bioecofunge-1" which , in addition to protecting …

New techs, new threats: sfide e opportunità della rivoluzione blockchain

2021

Considering the current diffusion of blockchain technology in the most different fields, ranging from public governance to economics, from contractual applications to health, the contribution addresses the issue of the legal regulation of this new technology, precisely because of its vast applications. From the comparison between the European model and the American one, the approach of the European legislator ‒ despite the delay with which the institutions have addressed the issue, seems to show, as already happened in other areas, a more 'social oriented' perspective and based on a sort of 'democratization' of data compared to the American approach, more ‘business oriented’, where the scho…

Genetic and Molecular Characterization of The Human Osteosarcoma 3AB-OS Cancer Stem Cell Line: A Possible Model For Studying Osteosarcoma Origin and …

2013

Finding new treatments targeting cancer stem cells (CSCs) within a tumor seems to be critical to halt cancer and improve patient survival. Osteosarcoma is an aggressive tumor affecting adolescents, for which there is no second-line chemotherapy. Uncovering new molecular mechanisms underlying the development of osteosarcoma and origin of CSCs is crucial to identify new possible therapeutic strategies. Here, we aimed to characterize genetically and molecularly the human osteosarcoma 3AB-OS CSC line, previously selected from MG63 cells and which proved to have both in vitro and in vivo features of CSCs. Classic cytogenetic studies demonstrated that 3AB-OS cells have hypertriploid karyotype wit…