Search results for "Replica"

The elemental role of iron in DNA synthesis and repair

2017

Iron is an essential redox element that functions as a cofactor in many metabolic pathways. Critical enzymes in DNA metabolism, including multiple DNA repair enzymes (helicases, nucleases, glycosylases, demethylases) and ribonucleotide reductase, use iron as an indispensable cofactor to function. Recent striking results have revealed that the catalytic subunit of DNA polymerases also contains conserved cysteine-rich motifs that bind iron–sulfur (Fe/S) clusters that are essential for the formation of stable and active complexes. In line with this, mitochondrial and cytoplasmic defects in Fe/S cluster biogenesis and insertion into the nuclear iron-requiring enzymes involved in DNA synthesis a…

Digital innovation & enterprise in the sharing economy: An action research agenda

2020

Abstract In this digital era, Digital Innovation & Enterprise have emerged as a possible prescription in the sharing economy. Scholars have noted that digital innovation is transforming the technological landscape, entrepreneurial practices, and the behaviours, attitudes, and beliefs of consumers across the globe. This research note serves three purposes. First, it introduces digital innovation and enterprise as a fruitful area of research in the sharing economy. Second, it extends the agenda set by Yoo, Henfridsson, and Lyytinen (2010) on “new organising logics of Digital Innovation” to propose contemporary research questions for scholarly collaboration. Third, it attempts to move forward …

Introducing SD modelling into planning and control systems to manage SMEs' growth: a learning-oriented perspective

2002

This article emphasises the need for a learning-oriented approach to planning and control as a prerequisite for SMEs’ survival and growth. Such a perspective can be effectively pursued through the development of interactive learning environments linking system dynamics (SD) and accounting models, which provide two complementary views of business phenomena. In order to properly support the drawing up of business plans and the evaluation of results associated with their implementation, the use of financial SD models embodying the accounting perspective is recommended. This is likely to enhance a shift in SME key actors’ minds, as they will be able to analyse under the feedback view financial …

Efficacy of screen recording in the other-revision of translations: episodic memory and event models

2014

In a 2011 study, Angelone compared the self-revision results of graduate German translation students. Participants documented their original translations using Integrated Problem and Decision Reporting (IPDR) logs (Gile 2004), think-aloud protocols and screen recordings. They then used this documentation to assist self-revision of their translations. Angelone found a significant improvement in error detection overall and in each of six discrete error categories when participants used screen recordings to assist their self-revision. We sought to partially replicate Angelone’s findings concerning the efficacy of screen recording in translation revision. Instead of focusing on self-revision, w…

Pragmatic markers resulting from language contact. The case of sañani in Aymara

2020

This paper explores the pragmatic functions of a previously unattested pragmatic marker (Fraser, 1996, 2006) found in Aymara, i.e. sanani ‘let’s say’. The uses of sanani suggest that this marker is the result of the influence of Spanish on Aymara due to sustained language contact. Sanani seems to be the “replication” (Heine and Kuteva, 2005) of the Spanish pragmatic marker digamos ‘let’s say’. Like digamos (Grande Alija, 2010; Quartararo, 2017a), sanani functions as a pragmatic marker by signaling either an inferential process or the semantic relation between two discourse segments. The original data used for this analysis was gathered through the Family Problems Picture task (San Roque et …

Detection of hepatitis C virus replication in ovarian metastases of a patient with hepatocellular carcinoma.

1994

Hepatocellular carcinoma is one of the most common human cancers with an annual incidence of about 1,000,000 cases worldwide. Although hepatocellular carcinoma is predominant in hepatitis B virus endemic areas, it has also become a major problem in Europe, Japan and North America in close association with the increasing incidence of hepatitis C virus infection. The pathogenetic role of hepatitis C virus infection in the development of HBsAg-negative hepatocellular carcinoma needs to be clarified. In this paper the case of a 66-year-old HBsAg-negative and anti-HCV positive female who developed hepatocellular carcinoma in a cirrhotic liver is reported. After 1 year of follow up, urgent laparo…

CHARACTERIZATION OF CHRONIC HEPATITIS B IN CHILDHOOD USING MOLECULAR BIOLOGY TECHNIQUES

1992

The introduction of molecular biology techniques in the diagnostics of chronic hepatitis B virus infection proved HBV DNA to be the most sensitive marker of viral replication and infectivity. The aim of our study was to characterize the HBV DNA status in children with chronic hepatitis B with various molecular biology techniques in relation to conventional HBV markers. Methods: 206 sera of 172 and liver tissue of 108 children with chronic hepatitis B infection were investigated by dot blot-, Southern blot-, and in situ hybridization. In dot blot and Southern blot negative specimens polymerase chain reaction (PCR) was performed. Results: 111 of the 206 sera were positive for HBV DNA by dot b…

Random, asynchronous, and asymmetric transcriptional activity of enhancer-flanking major immediate-early genes ie1/3 and ie2 during murine cytomegalo…

2001

ABSTRACT The lungs are a major organ site of cytomegalovirus (CMV) pathogenesis, latency, and recurrence. Previous work on murine CMV latency has documented a high load and an even distribution of viral genomes in the lungs after the resolution of productive infection. Initiation of the productive cycle requires expression of the ie1/3 transcription unit, which is driven by the immediate-early (IE) promoter P 1/3 and generates IE1 and IE3 transcripts by differential splicing. Latency is molecularly defined by the absence of IE3 transcripts specifying the essential transactivator protein IE3. In contrast, IE1 transcripts were found to be generated focally and randomly, reflecting sporadic P …

2014

The lungs are a noted predilection site of acute, latent, and reactivated cytomegalovirus (CMV) infections. Interstitial pneumonia is the most dreaded manifestation of CMV disease in the immunocompromised host, whereas in the immunocompetent host lung-infiltrating CD8 T cells confine the infection in nodular inflammatory foci and prevent viral pathology. By using murine CMV infection as a model, we provide evidence for a critical role of mast cells (MC) in the recruitment of protective CD8 T cells to the lungs. Systemic infection triggered degranulation selectively in infected MC. The viral activation of MC was associated with a wave of CC chemokine ligand 5 (CCL5) in the serum of C57BL/6 m…

Hepatitis C virus - associated B cell non-Hodgkin's lymphoma.

2016

The hepatitis C virus (HCV) infected patients are prone to develop bone marrow or various tissue infiltrates with monoclonal B cells, monoclonal B lymphocytosis or different types of B cell non-Hodgkin's lymphoma (BCNHL), of which the most common are splenic marginal zone BCNHL, diffuse large BCNHL and follicular lymphoma. The association between chronic HCV infection and non Hodgkin's lymphoma has been observed especially in areas with high prevalence of this viral infection. Outside the limitations of some studies that have been conducted, there are also geographic, environmental, and genetic factors that contribute to the epidemiological differences. Various microenvironmental signals, s…