Search results for "Research"

showing 10 items of 25967 documents

Zeolite-silver-zinc nanoparticles : biocompatibility and their effect on the compressive strength of mineral trioxide aggregate

2016

Background This study was carried out to evaluate the biocompatibility of zeolite-silver-zinc (Ze-Ag-Zn) nanoparticles and their effect on the compressive strength of Mineral Trioxide Aggregate (MTA). Material and Methods Biocompatibility was evaluated by an MTT assay on the pulmonary adenocarcinoma cells with 0.05, 0.1, 0.25, 0.5, 1 and 5 mg/mL concentrations of Ze-Ag-Zn. For compressive strength test, four groups containing 15 stainless-steel cylinders with an internal diameter of 4 and a height of 6 mm were prepared and MTA (groups 1 and 2) or MTA + 2% Ze-Ag-Zn (groups 3 and 4) were placed in the cylinders. The compressive strength was evaluated using a universal testing machine 4 days a…

0106 biological sciencesMineral trioxide aggregateUniversal testing machineBiocompatibilityChemistryResearchPulmonary adenocarcinomaMineralogy030206 dentistryZinc nanoparticles:CIENCIAS MÉDICAS [UNESCO]01 natural sciences03 medical and health sciences0302 clinical medicineCompressive strength010608 biotechnologyBiomaterials and Bioengineering in DentistryUNESCO::CIENCIAS MÉDICASMTT assayZeoliteGeneral DentistryNuclear chemistry
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Increasing temperature alters the within-host competition of viral strains and influences virus genetic variability

2021

Environmental conditions can affect viral accumulation, virulence and adaptation, which have implications in the disease outcomes and efficiency of control measures. Concurrently, mixed viral infections are relevant in plants, being their epidemiology shaped by within-host virus–virus interactions. However, the extent in which the combined effect of variations in abiotic components of the plant ecological niche and the prevalence of mixed infections affect the evolutionary dynamics of viral populations is not well understood. Here, we explore the interplay between ecological and evolutionary factors during viral infections and show that isolates of two strains of Pepino mosaic potexvirus co…

0106 biological sciencesMixed infectionsvirusesPlant virusVirulenceBiologyEvolutionary ecology01 natural sciencesMicrobiology03 medical and health sciencesplant virusenvironmental factorsVirologyPlant virusgenetic variabilityEnvironmental factorsAcademicSubjects/MED00860Genetic variabilityEvolutionary dynamics030304 developmental biologyGenetics0303 health sciencesGenetic diversityHost (biology)mixed infectionsAcademicSubjects/SCI01130AcademicSubjects/SCI02285evolutionary ecologyGenetic variabilityEvolutionary ecologyAdaptationResearch Article010606 plant biology & botany
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Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on biomineralization

2010

Abstract Background The shell of the pearl-producing bivalve Pinctada margaritifera is composed of an organic cell-free matrix that plays a key role in the dynamic process of biologically-controlled biomineralization. In order to increase genomic resources and identify shell matrix proteins implicated in biomineralization in P. margaritifera, high-throughput Expressed Sequence Tag (EST) pyrosequencing was undertaken on the calcifying mantle, combined with a proteomic analysis of the shell. Results We report the functional analysis of 276 738 sequences, leading to the constitution of an unprecedented catalog of 82 P. margaritifera biomineralization-related mantle protein sequences. Component…

0106 biological sciencesModels MolecularProteomicsProteome[SDV]Life Sciences [q-bio]Proteomics01 natural sciencesContig MappingMantle (mollusc)MargaritiferaIn Situ HybridizationGeneticsExpressed Sequence Tags0303 health sciencesMineralsbiologyPinctada margaritifera[ SDV.BBM.GTP ] Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN]ProteomeBiotechnologyResearch Articlelcsh:QH426-470Sequence analysislcsh:BiotechnologyMolecular Sequence Data010603 evolutionary biology03 medical and health sciencesCalcification Physiologiclcsh:TP248.13-248.65[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN]GeneticsAnimals[SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular BiologyAmino Acid SequencePinctadaRNA Messenger[SDV.IB.BIO]Life Sciences [q-bio]/Bioengineering/Biomaterials030304 developmental biologyBase SequenceSequence Homology Amino AcidGene Expression ProfilingAnimal StructuresMolecular Sequence AnnotationSequence Analysis DNAbiology.organism_classification[ SDV.IB.BIO ] Life Sciences [q-bio]/Bioengineering/Biomaterialslcsh:GeneticsGene Expression RegulationEvolutionary biologyPinctadaBiomineralization
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Bayesian analysis improves experimental studies about temporal patterning of aggression in fish.

2017

Made available in DSpace on 2018-12-11T17:15:13Z (GMT). No. of bitstreams: 0 Previous issue date: 2017-12-01 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) This study aims to describe a Bayesian Hierarchical Linear Model (HLM) approach for longitudinal designs in fish's experimental aggressive behavior studies as an alternative to classical methods In particular, we discuss the advantages of Bayesian analysis in dealing with combined variables, non-statistically significant results and required sample size using an experiment of angelfish (Pterophyllum scalare) species as case study. Groups of 3 individuals were subjected to daily observations recorded for 10 min durin…

0106 biological sciencesMonte Carlo methodBayesian probabilityBayesian analysisAquaculture010603 evolutionary biology01 natural sciencesStability (probability)Behavioral NeuroscienceStatisticsAnimals0501 psychology and cognitive sciences050102 behavioral science & comparative psychologyPterophyllum scalareProbabilitybiologyMarkov chain05 social sciencesMultilevel modelAggressive behaviorBayes TheoremGeneral MedicineCichlidsbiology.organism_classificationLongitudinal designMarkov ChainsAggressionVariable (computer science)Sample size determinationResearch DesignAnimal Science and ZoologyPsychologyMonte Carlo MethodBehavioural processes
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Going underground: short- and long-term movements may reveal the fossorial spatial ecology of an amphisbaenian

2021

[Background]: The movement and spatial ecology of an animal depends on its morphological and functional adaptations to its environment. In fossorial animals, adaptations to the underground life help to face peculiar ecological challenges, very different from those of epigeal species, but may constrain their movement ability.

0106 biological sciencesMovement patternsEcology (disciplines)010603 evolutionary biology01 natural sciencesAmphisbaenians03 medical and health sciencesSpace useTrogonophis wiegmannilcsh:QH301-705.5PIT tag telemetryEcology Evolution Behavior and Systematics030304 developmental biology0303 health sciencesMovement (music)EcologyResearchSpace useFossorialTerm (time)Geographylcsh:Biology (General)Animal ecologyFossorial reptilesSpatial ecologyEpigeal
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Efficient estimation of generalized linear latent variable models.

2019

Generalized linear latent variable models (GLLVM) are popular tools for modeling multivariate, correlated responses. Such data are often encountered, for instance, in ecological studies, where presence-absences, counts, or biomass of interacting species are collected from a set of sites. Until very recently, the main challenge in fitting GLLVMs has been the lack of computationally efficient estimation methods. For likelihood based estimation, several closed form approximations for the marginal likelihood of GLLVMs have been proposed, but their efficient implementations have been lacking in the literature. To fill this gap, we show in this paper how to obtain computationally convenient estim…

0106 biological sciencesMultivariate statisticsMultivariate analysisComputer scienceBinomials01 natural sciencesPolynomials010104 statistics & probabilityAmoebastilastolliset mallitestimointiProtozoansLikelihood FunctionsMultidisciplinaryApproximation MethodsStatistical ModelsSimulation and ModelingApplied MathematicsStatisticsQLinear modelREukaryotaLaplace's methodData Interpretation StatisticalPhysical SciencesVertebratesMedicineAlgorithmAlgorithmsResearch ArticleOptimizationScienceLatent variableResearch and Analysis Methods010603 evolutionary biologygeneralized linear latent variable modelsSet (abstract data type)BirdsAnimalsComputer Simulation0101 mathematicsta112OrganismsBiology and Life SciencesStatistical modelMarginal likelihoodAlgebraAmniotesMultivariate AnalysisLinear ModelsMathematicsSoftwarePLoS ONE
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Adaptation of turnip mosaic potyvirus to a specific niche reduces its genetic and environmental robustness

2020

Robustness is the preservation of the phenotype in the face of genetic and environmental perturbations. It has been argued that robustness must be an essential fitness component of RNA viruses owed to their small and compacted genomes, high mutation rates and living in ever-changing environmental conditions. Given that genetic robustness might hamper possible beneficial mutations, it has been suggested that genetic robustness can only evolve as a side-effect of the evolution of robustness mechanisms specific to cope with environmental perturbations, a theory known as plastogenetic congruence. However, empirical evidences from different viral systems are contradictory. To test how adaptation…

0106 biological sciencesMutation rateNicherobustness010603 evolutionary biology01 natural sciencesMicrobiologyGenome03 medical and health sciencesplant virusVirologythermal fluctuationsAcademicSubjects/MED00860experimental evolutionplastogenetic congruence030304 developmental biologyvirus evolution0303 health sciencesExperimental evolutionbiologyAcademicSubjects/SCI01130AcademicSubjects/SCI02285PotyvirusRobustness (evolution)biology.organism_classificationPhenotypeEvolutionary biologyViral evolutionmutagenesisResearch Article
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Natural Selection Fails to Optimize Mutation Rates for Long-Term Adaptation on Rugged Fitness Landscapes

2008

The rate of mutation is central to evolution. Mutations are required for adaptation, yet most mutations with phenotypic effects are deleterious. As a consequence, the mutation rate that maximizes adaptation will be some intermediate value. Here, we used digital organisms to investigate the ability of natural selection to adjust and optimize mutation rates. We assessed the optimal mutation rate by empirically determining what mutation rate produced the highest rate of adaptation. Then, we allowed mutation rates to evolve, and we evaluated the proximity to the optimum. Although we chose conditions favorable for mutation rate optimization, the evolved rates were invariably far below the optimu…

0106 biological sciencesMutation rateTime FactorsDigital organismsFitness landscapeQH301-705.5Biology010603 evolutionary biology01 natural sciencesCellular and Molecular Neuroscience03 medical and health sciences0302 clinical medicineGeneticsComputer SimulationBiology (General)Selection GeneticMolecular BiologyEcology Evolution Behavior and Systematics030304 developmental biology0303 health sciencesEvolutionary BiologyNatural selectionEcologyModels GeneticComputational Biology15. Life on landAdaptation PhysiologicalBiological EvolutionComputational Biology/Evolutionary ModelingReplication fidelityAsexual populationsEvolvabilityComputational Theory and MathematicsEvolutionary biologyModeling and SimulationViral evolutionMutation (genetic algorithm)MutationDNA Mismatch repairAdaptationAvida030217 neurology & neurosurgeryResearch Article
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Local and systemic mycorrhiza-induced protection against the ectoparasitic nematode Xiphinema index involves priming of defence gene responses in gra…

2012

International audience; The ectoparasitic dagger nematode (Xiphinema index), vector of Grapevine fanleaf virus (GFLV), provokes gall formation and can cause severe damage to the root system of grapevines. Mycorrhiza formation by Glomus (syn. Rhizophagus) intraradices BEG141 reduced both gall formation on roots of the grapevine rootstock SO4 (Vitis berlandierixV. riparia) and nematode number in the surrounding soil. Suppressive effects increased with time and were greater when the nematode was post-inoculated rather than co-inoculated with the arbuscular mycorrhizal (AM) fungus. Using a split-root system, decreased X. index development was shown in mycorrhizal and non-mycorrhizal parts of my…

0106 biological sciencesNematodaPhysiology[SDV]Life Sciences [q-bio]NepovirusPlant Science01 natural sciencesXiphinema indexPlant RootsGlomeromycota03 medical and health sciencesGene Expression Regulation PlantMycorrhizaeBotanyGallAnimalsVitisMycorrhizaGlomeromycotaGlomus030304 developmental biologyPlant DiseasesPlant Proteins2. Zero hunger0303 health sciencesbiologyarbuscular mycorrhizaGrapevine fanleaf virussplit-root systembiology.organism_classificationgrapevineNematode[SDE]Environmental Sciencesbioprotectionxiphinema indexdefence gene expressionRootstock010606 plant biology & botanyResearch Paper
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A new flatworm species of Temnocephala (Rhabdocoela, Temnocephalidae) ectosymbiont on the freshwater crab Valdivia serrata (Decapoda, Trichodactylida…

2020

A new species of temnocephalan is described from the branchial chambers ofValdivia serratain Colombia asTemnocephala ivandarioisp. nov.The most distinctive characters of the new species are in the cirrus and the epidermal ‘excretory’ syncytial plates. In the present study, the terminology to describe the cirrus of species ofTemnocephalais updated. Comparison between the shape of the cirrus of the temnocephalans associated with trichodactylid crabs is also provided.

0106 biological sciencesNot assignedTrichodactyloidearoyalty.order_of_chivalryroyaltyEubrachyuraTemnocephala01 natural sciencesEriphioideaEumalacostracataxonomyDecapodaCrustacealcsh:ZoologyBilaterialcsh:QL1-991TemnocephaloideaMalacostracaReserva Natural TanimbocabiologyDecapodaCephalornisRhabdocoelaTemnocephala ivandarioi sp. nov.Taxonomy (biology)CirrusEucaridaTemnocephalidaFreshwater crabCoelenterataResearch ArticlePortunoideaValdiviaArthropodaNephrozoa010607 zoologyProtostomiaZoologyDalytyphloplanidaTrichodactylidaeTemnocephalidaeCircumscriptional names of the taxon under010603 evolutionary biologyPolychelidaSystematicsHaplopharynx quadristimulusValdivia serrataAnimaliaEcology Evolution Behavior and SystematicsTrichodactylidaeFlatwormbiology.organism_classificationCrustaceanCyclodorippoideaRhabdocoelaNotchiaEcdysozoaAnimal Science and ZoologyPlatyhelminthesCrustacea Reserva Natural Tanimboca taxonomy Temnocephala ivandarioi sp. nov.AmericasZooKeys
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