Search results for "Retargeting"
showing 5 items of 15 documents
Characterization of poultry egg-white avidins and their potential as a tool in pretargeting cancer treatment.
2003
Chicken avidin and bacterial streptavidin are proteins used in a wide variety of applications in the life sciences due to their strong affinity for biotin. A new and promising use for them is in medical pretargeting cancer treatments. However, their pharmacokinetics and immunological properties are not always optimal, thereby limiting their use in these applications. To search for potentially beneficial new candidates, we screened egg white from four different poultry species for avidin. Avidin proteins, isolated from the duck, goose, ostrich and turkey, showed a similar tetrameric structure, similar glycosylation and stability against both temperature and proteolytic activity of proteinase…
Physical simulation for real-time image/video retargeting
2012
Retargeting methods present content on arbitrary aspect ratio me- dia displays limiting distortions in relevant objects. This is done by means of applying non-homogeneous resizing operators across the whole media, constraining it to fit into the required size. Several succesful systems have been proposed to achieve image retargeting, while video retargeting is still challenging due to time consistency and computational complexity requirements. Two important con- tributes were proposed: non-homogeneous retargeting [Wolf et al. 2007] and improved seam-carving [Rubinstein et al. 2008]. The first one claims to achieve real-time performance but considers spa- tial coordinates separately. The lat…
Saliency Based Aesthetic Cut of Digital Images
2013
Aesthetic cut of photos is a process well known to professional photographers. It consists of cutting the original photo to remove less relevant parts close to the borders leaving in this way the interesting subjects in a position that is perceived by the observer as more pleasant. In this paper we propose a saliency based technique to automatically perform aesthetic cut in images. We use a standard method to estimate the saliency map and propose some post processing on the map to make it more suitable for our scope. We then apply a greedy algorithm to determine the cut (i.e. the most important part of the original image) both in the cases of free and fixed aspect ratio. Experimental result…
Evaluation of the inverse electron demand Diels-Alder reaction in rats using a scandium-44-labelled tetrazine for pretargeted PET imaging
2019
Background Pretargeted imaging allows the use of short-lived radionuclides when imaging the accumulation of slow clearing targeting agents such as antibodies. The biotin-(strept)avidin and the bispecific antibody-hapten interactions have been applied in clinical pretargeting studies; unfortunately, these systems led to immunogenic responses in patients. The inverse electron demand Diels-Alder (IEDDA) reaction between a radiolabelled tetrazine (Tz) and a trans-cyclooctene (TCO)-functionalized targeting vector is a promising alternative for clinical pretargeted imaging due to its fast reaction kinetics. This strategy was first applied in nuclear medicine using an 111In-labelled Tz to image TC…
Trans -Cyclooctene-Functionalized PeptoBrushes with Improved Reaction Kinetics of the Tetrazine Ligation for Pretargeted Nuclear Imaging
2020
Tumor targeting using agents with slow pharmacokinetics represents a major challenge in nuclear imaging and targeted radionuclide therapy as they most often result in low imaging contrast and high radiation dose to healthy tissue. To address this challenge, we developed a polymer-based targeting agent that can be used for pretargeted imaging and thus separates tumor accumulation from the imaging step in time. The developed targeting agent is based on polypeptide-graft-polypeptoid polymers (PeptoBrushes) functionalized with trans-cyclooctene (TCO). The complementary In-111-labeled imaging agent is a 1,2,4,5-tetrazine derivative, which can react with aforementioned TCO-modified PeptoBrushes i…