Search results for "Retinal"
showing 10 items of 631 documents
AAV-Mediated Clarin-1 Expression in the Mouse Retina: Implications for USH3A Gene Therapy
2015
Usher syndrome type III (USH3A) is an autosomal recessive disorder caused by mutations in clarin-1 (CLRN1) gene, leading to progressive retinal degeneration and sensorineural deafness. Efforts to develop therapies for preventing photoreceptor cell loss are hampered by the lack of a retinal phenotype in the existing USH3 mouse models and by conflicting reports regarding the endogenous retinal localization of clarin-1, a transmembrane protein of unknown function. In this study, we used an AAV-based approach to express CLRN1 in the mouse retina in order to determine the pattern of its subcellular localization in different cell types. We found that all major classes of retinal cells express AAV…
Sodium channels enable fast electrical signaling and regulate phagocytosis in the retinal pigment epithelium
2019
Background Voltage-gated sodium (Nav) channels have traditionally been considered a trademark of excitable cells. However, recent studies have shown the presence of Nav channels in several non-excitable cells, such as astrocytes and macrophages, demonstrating that the roles of these channels are more diverse than was previously thought. Despite the earlier discoveries, the presence of Nav channel-mediated currents in the cells of retinal pigment epithelium (RPE) has been dismissed as a cell culture artifact. We challenge this notion by investigating the presence and possible role of Nav channels in RPE both ex vivo and in vitro. Results Our work demonstrates that several subtypes of Nav cha…
Photoreceptor vitality in organotypic cultures of mature vertebrate retinas validated by light-dependent molecular movements
2006
AbstractVertebrate photoreceptor cells are polarized neurons highly specialized for light absorption and visual signal transduction. Photoreceptor cells consist of the light sensitive outer segment and the biosynthetic active inner segment linked by a slender connecting cilium. The function of mature photoreceptor cells is strictly dependent on this compartmentalization which is maintained in the specialized retinal environment. To keep this fragile morphologic and functional composition for further cell biological studies and treatments we established organotypic retina cultures of mature mice and Xenopus laevis. The organotypic retina cultures of both model organisms are created as co-cul…
Small field motion detection in goldfish is red-green color blind and mediated by the M-cone type
2007
Large field motion detection in goldfish, measured in the optomotor response, is based on the L-cone type, and is therefore color-blind (Schaerer & Neumeyer, 1996). In experiments using a two-choice training procedure, we investigated now whether the same holds for the detection of a small moving object (size: 8 mm diameter; velocity: 7 cm/s). In initial experiments, we found that goldfish did not discriminate between a moving and a stationary stimulus, obviously not taking attention to the cue “moving.” Therefore, random dot patterns were used in which the stimulus was visible only when moving. Using black and white random dot patterns with variable contrast between 0.2 and 1, we found…
Validation of a method for measuring the retinal thickness with Shack–Hartmann aberrometry in an artificial eye
2015
In Shack–Hartmann aberrometry, it is assumed that a wave front emerges from a single point focused on a retina. However, the retina is a multi-layered structure and reflections may occur from several layers. This may result in several overlapping spot patterns on the CCD due to different vergences of the outgoing wave fronts. The amount by which these spot patterns are displaced may contain information about the retinal thickness. In this study, we perform simulations of formation of double spots in a living eye and also apply this method to measure the thickness of an artificial retina with a simple structure. We also compare the results obtained with artificial eye and compare them to the…
Magnification of the retinal image through an intraocular Galilean telescope
2010
The intraocular Galilean telescope is a good solution for incrementing the visual capabilities of patients with age-related macular degeneration. Nevertheless, some problems arise with the use of the intraocular telescopes available at present; these problems are originated by two main factors: the use of the same power values for the lenses in all types of eyes and the fact that the values of the distance between lenses into the eye are not the most suitable ones. As a result, a high residual refraction is produced in many implanted eyes. We propose a method for predicting outcomes in order to select patients for this technique and, moreover, for choosing the optimum power for the positive…
<title>Factors affecting intraocular light scattering from different color straylight sources</title>
2008
Important optical parameter of the eye is intraocular light scattering. Straylight can reduce visual acuity, contrast sensitivity. It is one of the main factors for glare, especially for drivers at night, when there is light source some distance away from the fixation point. There are many factors, which can affect amount of light scattering in the eye. To assess the effect of the color of the straylight source on retinal image quality at different light scattering levels, retinal straylight was measured with and without light scattering occluder. Red, green and blue colors were choosed for straylight source. Psychophysical and electrophysiological methods were used to evaluate light scatte…
Erhöhte Schwellen für die Detektion von Phasenunterschieden in musterinduzierten Flimmerfarben bei Patienten mit Glaukom
1994
BACKGROUND: Pattern-induced flicker-colors are subjective colors produced by rotating disks with black-and-white arcs. Changing the direction of rotation results in a different color. Small variations in the temporal sequence (phase differences) of the black-and-white pattern cause the colors to vary and can be perceived as color differences. PATIENTS AND METHODS: We investigated the thresholds for such phase differences in healthy (n = 11) and in glaucomatous (n = 19) eyes. Acuity and color vision were tested and in patients static visual field perimetry (Octopus G1) was carried out. The disks used for producing the pattern-induced flicker-colors were viewed from a distance of 2 m and had …
A Comparative Study on Feature Selection for Retinal Vessel Segmentation Using FABC
2009
This paper presents a comparative study on five feature selection heuristics applied to a retinal image database called DRIVE. Features are chosen from a feature vector (encoding local information, but as well information from structures and shapes available in the image) constructed for each pixel in the field of view (FOV) of the image. After selecting the most discriminatory features, an AdaBoost classifier is applied for training. The results of classifications are used to compare the effectiveness of the five feature selection methods.
Unsupervised recognition of retinal vascular junction points.
2014
Landmark points in retinal images can be used to create a graph representation to understand and to diagnose not only different pathologies of the eye, but also a variety of more general diseases. Aim of this paper is the description of a non-supervised methodology to distinguish between bifurcations and crossings of the retinal vessels, which can be used in differentiating between arteries and veins. A thinned representation of the binarized image, is used to identify pixels with three or more neighbors. Junction points are classified into bifurcations or crossovers according to their geometrical and topological properties. The proposed approach is successfully compared with the state-of-t…