Search results for "Roca"

showing 10 items of 1893 documents

Re-expression of C/EBP alpha induces CYP2B6, CYP2C9 and CYP2D6 genes in HepG2 cells.

1998

Cytochrome P450 (CYP) activity is very low or even absent in human hepatomas, a phenomenon that is accompanied by low levels of some liver transcription factors, notably C/EBP alpha. To investigate a possible link between this transcription factor and hepatic CYP expression, we have stably transfected HepG2 cells with a C/EBP alpha vector containing a Zn-inducible metallothionein promoter. Expression of functional C/EBP alpha up to liver levels concomitantly increased the mRNAs of several members of the CYP2 family (2B6, 2C9 and 2D6), suggesting that this transcription factor may play a relevant role in controlling the hepatic expression of CYP enzymes.

Carcinoma HepatocellularCYP2B6BiophysicsHepG2 cellTransfectionBiochemistryGene Expression Regulation EnzymologicCytochrome P-450 Enzyme SystemStructural BiologyTumor Cells CulturedGeneticsHumansMetallothioneinRNA MessengerVector (molecular biology)Molecular BiologyTranscription factorGeneCells CulturedCytochrome P-450 CYP2C9biologyChemistryNuclear ProteinsCytochrome P450Oxidoreductases N-DemethylatingCell BiologyTransfectionMolecular biologyDNA-Binding ProteinsCytochrome P-450 CYP2B6C/EBPαCytochrome P-450 CYP2D6Steroid 16-alpha-HydroxylaseHepatocyte nuclear factor 4 alphaEnzyme InductionSteroid HydroxylasesCCAAT-Enhancer-Binding Proteinsbiology.proteinAryl Hydrocarbon HydroxylasesHuman hepatocyteCytochrome P450 gene regulationTranscription Factors
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Focus sui non responder alla terapia di resincronizzazione cardiaca: orizzonti e prospettive

2017

Cardiac resynchronization therapy (CRT) has been shown as a successful strategy in the treatment of patients with heart failure and electrical dyssincrony. However, a significant proportion of implanted patients fails to respond sufficiently or in a predictable manner. Consequently, non response to CRT remains a valuable problem in clinical practice. In order to improve CRT response and long-term clinical benefits, the proper evaluation of patient's global frialty, the technology improvement, the multimodality imaging approach and the use of simple and low cost electrographic parameters (to verify effective biventricular capture and QRS narrowing) could play a important role. Therefore, the…

Cardiac Resynchronization TherapyHeart FailureElectrocardiographyTreatment OutcomePrognosiMedicine (all)Non responderMultimodal ImagingHuman
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Electrocardiographic Imaging for Atrial Fibrillation: A Perspective From Computer Models and Animal Experiments to Clinical Value

2021

[EN] Electrocardiographic imaging (ECGI) is a technique to reconstruct non-invasively the electrical activity on the heart surface from body-surface potential recordings and geometric information of the torso and the heart. ECGI has shown scientific and clinical value when used to characterize and treat both atrial and ventricular arrhythmias. Regarding atrial fibrillation (AF), the characterization of the electrical propagation and the underlying substrate favoring AF is inherently more challenging than for ventricular arrhythmias, due to the progressive and heterogeneous nature of the disease and its manifestation, the small volume and wall thickness of the atria, and the relatively large…

Cardiac arrhythmiasmedicine.medical_specialtyVOLUME CONDUCTORPhysiologymedicine.medical_treatmentROTORSCatheter ablationReview030204 cardiovascular system & hematologyTECNOLOGIA ELECTRONICA03 medical and health sciencesPERSISTENT0302 clinical medicinePhysiology (medical)Internal medicineMAGNETIC-RESONANCEmedicineQP1-981PULMONARY VEIN ISOLATIONSistema cardiovascularImatges tridimensionals en medicinabusiness.industrySmall volumeECGAF characterizationPerspective (graphical)Electrocardiographic imagingAtrial fibrillationmedicine.diseaseAtrial fibrillationDOMINANT FREQUENCYKey factorsElectrocardiographic imagingClinical valueCardiologyEnginyeria biomèdicaCatheter ablationbusinessWall thicknessTORSO INHOMOGENEITIESINVERSE PROBLEMInverse solutionTreatment guidance030217 neurology & neurosurgery
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Doxorubicin induces wide-spread transcriptional changes in the myocardium of hearts distinguishing between mice with preserved and impaired cardiac f…

2021

Abstract Aims Doxorubicin (DOX) is an important drug for the treatment of various tumor entities. However, the occurrence of heart failure limits its application. This study investigated differential gene expression profiles in the left and right ventricles of DOX treated mice with either preserved or impaired myocardial function. We provide new mechanistic insights into the pathophysiology of DOX-induced heart failure and have discovered pathways that counteract DOX-induced cardiotoxicity. Main methods We used in total 48 male mice and applied a chronic low dose DOX administration (5 mg/kg per injection, in total 20 mg/kg over 4 weeks) to induce heart failure. Echocardiographic parameters …

Cardiac function curveTranscription GeneticPharmacologymedicine.disease_causeGeneral Biochemistry Genetics and Molecular BiologyElectrocardiographypolycyclic compoundsmedicineAnimalsCluster AnalysisDoxorubicinGeneral Pharmacology Toxicology and Pharmaceuticschemistry.chemical_classificationReactive oxygen speciesCardiotoxicitybusiness.industryGene Expression ProfilingMyocardiumGeneral Medicinemedicine.diseasePathophysiologyMice Inbred C57BLGene expression profilingOxidative StresschemistryDoxorubicinHeart failureHeart Function TestsbusinessOxidative stressmedicine.drugLife Sciences
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Plasma Hyperosmolality Prolongs QTc Interval and Increases Risk for Atrial Fibrillation in Traumatic Brain Injury Patients

2020

Introduction: Hyperosmotic therapy with mannitol is frequently used for treatment cerebral edema, and 320 mOsm/kg H2O has been recommended as a high limit for therapeutic plasma osmolality. However, plasma hyperosmolality may impair cardiac function, increasing the risk of cardiac events. The aim of this study was to analyze the relation between changes in plasma osmolality and electrocardiographic variables and cardiac arrhythmia in patients treated for isolated traumatic brain injury (iTBI). Methods: Adult iTBI patients requiring mannitol infusion following cerebral edema, and with a Glasgow Coma Score below 8, were included. Plasma osmolality was measured with Osmometr 800 CLG. Spatial Q…

Cardiac function curvemedicine.medical_specialtyTraumatic brain injuryosmolar gapelectrocardiographylcsh:Medicine030204 cardiovascular system & hematologyQT intervalArticleCerebral edema03 medical and health sciences0302 clinical medicineInternal medicinemedicine030212 general & internal medicinecardiovascular diseasesbusiness.industrytraumatic brain injurylcsh:RGlasgow Coma ScaleCardiac arrhythmiaAtrial fibrillationGeneral Medicinemedicine.diseaseplasma osmolalityPlasma osmolalitycardiac arrhythmiasCardiologycardiovascular systembusinessJournal of Clinical Medicine
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An integrated approach based on uniform quantization for the evaluation of complexity of short-term heart period variability: Application to 24 h Hol…

2007

We propose an integrated approach based on uniform quantization over a small number of levels for the evaluation and characterization of complexity of a process. This approach integrates information-domain analysis based on entropy rate, local nonlinear prediction, and pattern classification based on symbolic analysis. Normalized and non-normalized indexes quantifying complexity over short data sequences (∼300 samples) are derived. This approach provides a rule for deciding the optimal length of the patterns that may be worth considering and some suggestions about possible strategies to group patterns into a smaller number of families. The approach is applied to 24 h Holter recordings of …

Cardiac outputDaytimeCardiac Output LowGeneral Physics and AstronomyRisk AssessmentSensitivity and SpecificityPhysics and Astronomy (all)Heart RateReference ValuesRisk FactorsOscillometryStatisticsHeart ratemedicineMathematical PhysicHumansCircadian rhythmDiagnosis Computer-AssistedMathematical PhysicsEntropy rateMathematicsmedicine.diagnostic_testApplied MathematicsReproducibility of ResultsStatistical and Nonlinear PhysicsSignal Processing Computer-AssistedIntegrated approachmedicine.diseasePrognosisSystems IntegrationHeart failureElectrocardiography AmbulatoryAlgorithmElectrocardiographyAlgorithmsStatistical and Nonlinear PhysicChaos (Woodbury, N.Y.)
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[Diagnosis of coronary microvascualar dysfunction in diabetic patients with cardiac syndrome X: comparison by current methods].

2013

The study population included 208 patients with chest pain and uninjured coronary arteries that we split into two populations: diabetics (72 patients) and non-diabetics (136 patients). We split patients with chest pain and uninjured coronary arteries that had a myocardial scintigraphy into two populations: patients with positive scintigraphy and negative. We calculated, on angiographic images of each patient, stored on suitable digital supports, Timi Frame Count (TFC), Myocardial Blush Grade (MBG) and Total Myocardial Blush Score (TMBS) using the protocol described by Gibson and Yusuf. On the basis of Yusuf's experience we imagined a new index: the Total Timi Frame Count as the sum of the t…

Cardiac syndrome X chest pain coronary angiography diabetes mellitus microcirculation myocardial scintigraphyChest PainMicrocirculationReproducibility of ResultsCoronary DiseaseCoronary AngiographySeverity of Illness IndexElectrocardiographyPredictive Value of TestsRisk FactorsCoronary CirculationExercise TestHumansEmergency Service HospitalRadionuclide ImagingBlood Flow VelocityDiabetic AngiopathiesMicrovascular Angina
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Modified electrocardiograph-triggered black-blood turbo spin-echo technique to improve T1-weighting in contrast-enhanced MRI of atherosclerotic carot…

2008

Purpose To assess the efficacy of a modified electrocardiograph (EKG)-triggered black-blood T1W (T1W) spin-echo sequence in improving contrast on post-gadolinium high-resolution carotid plaque imaging by implementing heart-rate–independent contrast preparation. Materials and Methods We used a standard EKG-triggered double inversion-recovery (DIR) turbo spin-echo (TSE) sequence modified with the addition of an extra saturation (90°) radio frequency (RF) pulse placed immediately after the DIR module, shortening the repetition time to a fixed value of 400 msec. A total of 10 patients with atherosclerotic disease were included in the study. Postinjection intraplaque contrast measurements were p…

Carotid Artery DiseasesMaleCONTRAST ENHANCED MRIImage qualityCarotid arteriesmedia_common.quotation_subjectBlack blood[INFO.INFO-IM] Computer Science [cs]/Medical ImagingContrast Media030218 nuclear medicine & medical imagingElectrocardiography03 medical and health sciencesMeglumine0302 clinical medicineImage Interpretation Computer-AssistedOrganometallic Compounds[INFO.INFO-IM]Computer Science [cs]/Medical ImagingHumansContrast (vision)MedicineRadiology Nuclear Medicine and imagingAgedmedia_commonPulse (signal processing)business.industryMiddle AgedFast spin echoMagnetic Resonance ImagingWeightingFemaleArtifactsbusinessNuclear medicine030217 neurology & neurosurgery
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Role of cAMP in mediating AHR signaling.

2009

Regulation of the nuclear import of many transcription factors represents a step in gene regulation which is crucial for a number of cellular processes. The aryl hydrocarbon receptor (AHR), a basic helix-loop-helix protein of the PAS (PER-ARNT-SIM) family of transcriptional regulators is a cytosol-associated and ligand-activated receptor. The environmental toxin dioxin binds with high affinity to AHR rendering it nuclear and leading to the activation of AHR sensitive genes. However, the fact, that the AHR mediates a large variety of physiological events without the involvement of any known exogenous ligand, including liver and vascular system development, maturation of the immune system, re…

Cellular differentiationNuclear translocationSignal transductionDioxinsLigandsBiochemistryCell LineProtein kinase ACyclic AMPCytochrome P-450 CYP1A1AnimalsHumansPhosphorylationReceptorProtein kinase ATranscription factorAryl hydrocarbon receptorPharmacologyRegulation of gene expressionbiologyAryl hydrocarbon receptorCyclic AMP-Dependent Protein KinasesProtein TransportBiochemistryReceptors Aryl HydrocarbonSecond messenger systembiology.proteinEnvironmental PollutantsSignal transductionDioxin toxicitySignal TransductionBiochemical pharmacology
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Natural fluorapatite as a raw material for Portland clinker

2018

Abstract The present work focuses on the mineralogy and the reactivity of clinkers made from the integration of natural fluorapatite in the raw meals with percentages ranging from 0 up to15%. The samples were characterized by infrared spectrometry, X-ray diffraction and microscopy techniques. The distribution of phosphorous and fluorine into clinkers minerals was determined by MEB/EDS mapping. The mineralogical composition was determined by XRD/Rietveld and the samples reactivity followed by isothermal microcalorimetry. It has been found that fluorine stabilizes C3S and β-C2S which still are found with high levels of P2O5. Cements obtained from up to 8% natural fluorapatite incorporation st…

CementIsothermal microcalorimetryMaterials scienceMetallurgyFluorapatite0211 other engineering and technologieschemistry.chemical_elementInfrared spectroscopy02 engineering and technologyBuilding and ConstructionRaw material021001 nanoscience & nanotechnologyClinker (cement)chemistry021105 building & constructionFluorineGeneral Materials ScienceReactivity (chemistry)0210 nano-technologyCement and Concrete Research
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