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showing 10 items of 9311 documents

Thioredoxin (Trxo1) interacts with proliferating cell nuclear antigen (PCNA) and its overexpression affects the growth of tobacco cell culture.

2017

Thioredoxins (Trxs), key components of cellular redox regulation, act by controlling the redox status of many target proteins, and have been shown to play an essential role in cell survival and growth. The presence of a Trx system in the nucleus has received little attention in plants, and the nuclear targets of plant Trxs have not been conclusively identified. Thus, very little is known about the function of Trxs in this cellular compartment. Previously, we studied the intracellular localization of PsTrxo1 and confirmed its presence in mitochondria and, interestingly, in the nucleus under standard growth conditions. In investigating the nuclear function of PsTrxo1 we identified proliferati…

0106 biological sciences0301 basic medicineTFs transcription factorsOverexpressionBiologíaBiFC bimolecular fluorescence complementationClinical BiochemistryCell Culture TechniquesTobacco BY-2 cells01 natural sciencesBiochemistryTBY-2 tobacco bright yellow-2DTT 14-dithiothreitolBimolecular fluorescence complementationThioredoxinsGene Expression Regulation PlantTrx thioredoxinlcsh:QH301-705.5GFP green fluorescent proteinlcsh:R5-920biologyProliferating cell nuclear antigen (PCNA)Cell cycleGlutathione3. Good healthCell biologyMitochondriaNTR NADPH thioredoxin reductaseProtein TransportDEM diethyl maleateRT-qPCR Reverse transcription quantitative polymerase chain reactionThioredoxinlcsh:Medicine (General)Oxidation-ReductionAMS 4-acetamido-4-maleimidylstilbene-22-disulfonic acidResearch PaperPCNA proliferating cell nuclear antigenOex overexpressingCell cycleNucleusThioredoxin o103 medical and health sciencesROS reactive oxygen speciesDownregulation and upregulationProliferating Cell Nuclear AntigenTobaccoDAPI 46-diamidine-2-phenylindolmCBM monochlorobimaneCellular compartmentCell NucleusCell growthOrganic ChemistryBotánicaPeasMolecular biologyYFP yellow fluorescent proteinProliferating cell nuclear antigenTBS Tris-buffered salineOD optical density030104 developmental biologylcsh:Biology (General)Cell cultureRNA reactive nitrogen speciesbiology.proteinPrx peroxiredoxinBSA bovine serum albumin010606 plant biology & botanyRedox biology
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Marine Cryptophytes Are Great Sources of EPA and DHA

2017

Microalgae have the ability to synthetize many compounds, some of which have been recognized as a source of functional ingredients for nutraceuticals with positive health effects. One well-known example is the long-chain polyunsaturated fatty acids (PUFAs), which are essential for human nutrition. Eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) are the two most important long-chain omega-3 (-3) PUFAs involved in human physiology, and both industries are almost exclusively based on microalgae. In addition, algae produce phytosterols that reduce serum cholesterol. Here we determined the growth rates, biomass yields, PUFA and sterol content, and daily gain of eight strains of marine…

0106 biological sciences0301 basic medicineTROPICAL AUSTRALIAN MICROALGAELINOLENIC ACIDrasvahapotPharmaceutical Sciencesterols01 natural scienceschemistry.chemical_compoundFunctional FoodDrug DiscoveryFood scienceBiomasslcsh:QH301-705.5Pharmacology Toxicology and Pharmaceutics (miscellaneous)functional foodsPOLYUNSATURATED FATTY-ACIDSchemistry.chemical_classificationnutraceuticalsFRESH-WATERPRODUCTIVITYbiologymicroalgae; polyunsaturated fatty acids; omega-3; omega-6; sterols; functional foods; nutraceuticalsCHOLESTEROLmicroalgaeNANNOCHLOROPSISPhytosterolsfood and beveragesEicosapentaenoic acidEicosapentaenoic AcidDocosahexaenoic acidFatty Acids Unsaturatedlipids (amino acids peptides and proteins)omega-3CryptophytaPolyunsaturated fatty acidpolyunsaturated fatty acidsDocosahexaenoic Acidsomega-6CHEMICAL-COMPOSITIONterveysvaikutteiset elintarvikkeetfatty acidsGas Chromatography-Mass SpectrometryArticleMARICULTURE03 medical and health sciencesNutraceuticalAlgaeFatty Acids Omega-6Fatty Acids Omega-31172 Environmental sciencessterolit010604 marine biology & hydrobiologyomega fatty acidsta1183ta1182GROWTH-RATEmikrolevätbiology.organism_classificationSterolomegarasvahapot030104 developmental biologyHuman nutrition416 Food Sciencelcsh:Biology (General)chemistry13. Climate actionDietary SupplementsStearidonic acidMarine Drugs; Volume 16; Issue 1; Pages: 3
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UV-screening and springtime recovery of photosynthetic capacity in leaves of Vaccinium vitis-idaea above and below the snow pack

2019

International audience; Evergreen plants in boreal biomes undergo seasonal hardening and dehardening adjusting their photosynthetic capacity and photoprotection; acclimating to seasonal changes in temperature and irradiance. Leaf epidermal ultraviolet (UV)-screening by flavonols responds to solar radiation, perceived in part through increased ultraviolet-B (UV-B) radiation, and is a candidate trait to provide cross-photoprotection. At Hyytiälä Forestry Station, central Finland, we examined whether the accumulation of flavonols was higher in leaves of Vaccinium vitis-idaea L. growing above the snowpack compared with those below the snowpack. We found that leaves exposed to colder temperature…

0106 biological sciences0301 basic medicineTime FactorsPhotoinhibitionBOREALPhysiologyPlant ScienceForests01 natural sciencesPlant EpidermisAnthocyaninsSoilFlavonolsLOW-TEMPERATURESnowPhotosynthesis1183 Plant biology microbiology virologychemistry.chemical_classificationspring dehardening.CLIMATE-CHANGEbiologyChemistryTemperatureUnderstoreyHorticultureLIGHTSeasonsVacciniumUltraviolet RaysGrowing seasonPhotosynthesisDWARF SHRUB03 medical and health sciencesLEAFPHOTOSYSTEM-IIGenetics[SDV.BV]Life Sciences [q-bio]/Vegetal BiologyVaccinium vitis-idaeaFlavonoidsSpring dehardeningPhotoprotectionSpectral qualityPhotosystem II Protein ComplexPigments Biological15. Life on landEvergreenbiology.organism_classificationPhotosynthetic capacitySUB-ARCTIC HEATHPlant Leaves030104 developmental biology13. Climate actionPhotoprotectionWINTERB RADIATIONArctic browning010606 plant biology & botany
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Begomoviruses Infecting Tomato Crops in Panama

2015

The key regions in Panama involved in open field- and greenhouse-grown commercial tomato production, including the Chiriquí, Veraguas, Herrera, Los Santos, Coclé and Panama Oeste provinces, were surveyed for the incidence and distribution of begomoviruses in the growing seasons of 2011 and 2012. The surveys took place in 14 of the 51 districts of the above-mentioned provinces and comprised all relevant tomato production areas of the provinces. A total of 28 tomato plots were surveyed. The exact location of each plot was geo-referenced using a hand-held Global Positioning System unit. In total, 319 individual tomato plants (181 in 2011 and 138 in 2012) were sampled. Plants displayed diverse …

0106 biological sciences0301 basic medicineVeterinary medicinePhysiologyGrowing seasonTomato leaf curl Sinaloa viruPlant ScienceBiology01 natural scienceslaw.invention03 medical and health sciencesSolanum lycopersicumlawGenusBotanyTomato yellow mottle viruGeneticsLeaf sizePolymerase chain reactionPanamafungiBegomovirusSettore AGR/12 - Patologia Vegetalefood and beveragesbiology.organism_classificationPolymerase chain reactionTomato yellow mottle virus030104 developmental biologyKey (lock)Potato yellow mosaic Panama viruAgronomy and Crop Science010606 plant biology & botanyJournal of Phytopathology
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Boosting Biomass Quantity and Quality by Improved Mixotrophic Culture of the Diatom Phaeodactylum tricornutum

2021

Diatoms are photoautotrophic unicellular algae and are among the most abundant, adaptable, and diverse marine phytoplankton. They are extremely interesting not only for their ecological role but also as potential feedstocks for sustainable biofuels and high-value commodities such as omega fatty acids, because of their capacity to accumulate lipids. However, the cultivation of microalgae on an industrial scale requires higher cell densities and lipid accumulation than those found in nature to make the process economically viable. One of the known ways to induce lipid accumulation in Phaeodactylum tricornutum is nitrogen deprivation, which comes at the expense of growth inhibition and lower c…

0106 biological sciences0301 basic medicine[SDV.BIO]Life Sciences [q-bio]/BiotechnologyPhotobioreactorBiomassPlant Sciencelcsh:Plant culture01 natural sciences03 medical and health sciencesAlgaemixotrophic growthgenome-scale metabolic modelSettore BIO/04 - Fisiologia Vegetalelcsh:SB1-1110Phaeodactylum tricornutumbiomass productivityOriginal ResearchbiologyChemistryPlinear programmingbiology.organism_classificationPulp and paper industryP. tricornutumdiatomLight intensity030104 developmental biologyDiatomtricornutumBiofuelmetabolismMixotroph010606 plant biology & botanyFrontiers in Plant Science
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Geometric analysis of intrusive growth of wood fibres in Robinia pseudoacacia

2018

ABSTRACT All cell types of the secondary xylem arise from the meristematic cells (initials) of the vascular cambium and grow under mechanical constraints emerging from the circular-symmetrical geometry that characterises many tree trunks. The course of intrusive growth of cambial initials has been elucidated, but is yet to be described in the case of xylem fibres. This study explains the geometry of intrusive growth of the secondary xylem fibres in the trunk of Robinia pseudoacacia. Long series of serial semi-thin sections of the vascular cambium and the differentiating secondary xylem were analysed. Since fibres grow in close vicinity to expanding cells of the derivatives of the vascular c…

0106 biological sciences0301 basic medicinebiologyfungiAxial parenchymaRobiniaXylemForestryPlant ScienceMeristembiology.organism_classification01 natural sciences03 medical and health sciences030104 developmental biologyVascular cambiumBiophysicsAxial growthCambiumProcess (anatomy)010606 plant biology & botanyIAWA Journal
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Gene flow from an adaptively divergent source causes rescue through genetic and demographic factors in two wild populations of Trinidadian guppies

2016

Genetic rescue, an increase in population growth owing to the infusion of new alleles, can aid the persistence of small populations, but its use as a management tool is limited by a lack of empirical data geared towards predicting effects of gene flow on local adaptation and demography. Experimental translocations provide an ideal opportunity to monitor the demographic consequences of gene flow. In this study we take advantage of two experimental introductions of Trinidadian guppies to test the effects of gene flow on downstream native populations. We individually marked guppies from the native populations to monitor population dynamics for 3 months before and 26 months after gene flow. We …

0106 biological sciences0301 basic medicinecapture-mark-recaptureGenetic genealogyPoecilia reticulataPopulationdemographic rescueBiology010603 evolutionary biology01 natural sciencesGene flow03 medical and health sciencesgenetic rescueddc:570GeneticsAlleleeducationhybridizationEcology Evolution Behavior and SystematicsLocal adaptationGeneticseducation.field_of_studycapture‐mark‐recaptureEcotypePopulation sizeSmall population sizeOriginal Articlesfitness030104 developmental biologyväestönkasvuEvolutionary biologypopulation growthta1181Original ArticleGeneral Agricultural and Biological Sciencesgene flowEvolutionary Applications
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The In Vitro Interaction of 12-Oxophytodienoic Acid and Related Conjugated Carbonyl Compounds with Thiol Antioxidants

2021

α,β-unsaturated carbonyls interfere with numerous plant physiological processes. One mechanism of action is their reactivity toward thiols of metabolites like cysteine and glutathione (GSH). This work aimed at better understanding these interactions. Both 12-oxophytodienoic acid (12-OPDA) and abscisic acid (ABA) conjugated with cysteine. It was found that the reactivity of α,β-unsaturated carbonyls with GSH followed the sequence trans-2-hexenal &lt

0106 biological sciences0301 basic medicinecysteine covalent modification570Isomerase activityArabidopsis thalianaArabidopsislcsh:QR1-50201 natural sciencesBiochemistryArticleAntioxidantslcsh:Microbiology03 medical and health scienceschemistry.chemical_compoundThioredoxinsPlant Growth RegulatorsmedicineCysteineSulfhydryl CompoundsMolecular BiologyCyclophilinchemistry.chemical_classificationChemistry<i>Arabidopsis thaliana</i>peroxiredoxinGlutathionethioredoxinphytohormones030104 developmental biologyMechanism of actionBiochemistryprotein–ligand interactioncyclophilinThiolFatty Acids Unsaturatedmedicine.symptomThioredoxinPeroxiredoxinthiol antioxidants010606 plant biology & botanyCysteineBiomolecules
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Soil microbiome of primeval forest ecosystems in Transkarpathia

2018

The aim of this study was to investigate the soil microbiome of primeval forest ecosystems, namely the structure of microbial communities,the number of major ecological-functional groups, functional parameters such as: soil toxicity, as well as enzymatic activity of the soil by the level of catalase and invertase. To analyze the successional processes in the soil microbiocenosis due to the influence of endogenous and exogenous factors. To estimate the integrity of microbial communities in different edaphotopes of primeval forest ecosystems. Methods. Microbiological studies of soil were carried out according to generally accepted methods in soil microbiology. Enzymatic activity of the soil: …

0106 biological sciences0301 basic medicineecosystemgeographygeography.geographical_feature_categoryEcologyintegrity of communitiesenzymatic activityGeneral MedicineOld-growth forest010603 evolutionary biology01 natural sciencesprimeval forestsoil03 medical and health sciences030104 developmental biologyEnvironmental scienceEcosystemMicrobiomemicroorganismsMikrobiologičnij Žurnal
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Broad thermal tolerance is negatively correlated with virulence in an opportunistic bacterial pathogen

2018

Predicting the effects of global increase in temperatures on disease virulence is challenging, especially for environmental opportunistic bacteria, because pathogen fitness may be differentially affected by temperature within and outside host environment. So far, there is very little empirical evidence on the connections between optimal temperature range and virulence in environmentally growing pathogens. Here, we explored whether the virulence of an environmentally growing opportunistic fish pathogen, Flavobacterium columnare, is malleable to evolutionary changes via correlated selection on thermal tolerance. To this end, we experimentally quantified the thermal performance curves (TPCs) f…

0106 biological sciences0301 basic medicinelämmönsietoGrowing seasonVirulenceZoology010603 evolutionary biology01 natural sciencesthermal tolerancethermal performance curvesbakteerit03 medical and health sciencesOpportunistic pathogenGeneticsPathogenEcology Evolution Behavior and Systematics2. Zero hungerGenetic diversitybiologyHost (biology)opportunistic pathogenta1182virulenssiOriginal Articlesilmastonmuutoksetbiology.organism_classificationvirulenceclimate changetaudinaiheuttajat030104 developmental biology13. Climate actionFlavobacterium columnareta1181Original ArticleGeneral Agricultural and Biological SciencesBacteriaEvolutionary Applications
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