Search results for "Séquençage"
showing 10 items of 35 documents
La sensibilité des communautés microbiennes à une augmentation de la température augmente quand la biodégradabilité de la ressource organique baisse
2014
La sensibilité des communautés microbiennes à une augmentation de la température augmente quand la biodégradabilité de la ressource organique baisse. 12. Journées d'Etude des Sols
Changements à court terme de la composition des communautés de champignons mycorhiziens à arbuscules et des populations de Rhizophagus irregularis en…
2014
La mycorhize à arbuscule a un important potentiel d’amélioration de la nutrition minérale des plantes, fort utile dans le contexte agricole. Cependant, certaines pratiques culturales sont connues pour avoir un effet néfaste sur la diversité des champignons mycorhiziens à arbuscules (Glomeromycètes). Il est donc nécessaire de mieux comprendre les dynamiques de leurs communautés et populations pour pouvoir préserver et gérer le potentiel symbiotique. Dans cette étude, nous avons étudié les effets séparés et conjoints du retournement du sol et des fertilisations phosphatée et azotée du sol sur la structure des communautés des Glomeromycètes et sur les populations de l’espèce Rhizophagus irregu…
Single-cell RNA sequencing unveils the shared and the distinct cytotoxic hallmarks of human TCRVδ1 and TCRVδ2 γδ T lymphocytes
2019
γδ T lymphocytes represent ∼1% of human peripheral blood mononuclear cells and even more cells in most tissues of vertebrates. Although they have important anticancer functions, most current single-cell RNA sequencing (scRNA-seq) studies do not identify γδ T lymphocytes because their transcriptomes at the single-cell level are unknown. Here we show that high-resolution clustering of large scRNA-seq datasets and a combination of gene signatures allow the specific detection of human γδ T lymphocytes and identification of their T cell receptor (TCR)Vδ1 and TCRVδ2 subsets in large datasets from complex cell mixtures. In t -distributed stochastic neighbor embedding plots from blood and tumor sa…
Identification of molecular and physiopathologic basis in oral-facial-digital syndromes
2016
Oral-facial-digital syndromes (OFDS) are characterized by the association of oral, facial and digital anomalies. The different modes of inheritance and additional features lead to clinically delineate 13 subtypes. For a long time, only the OFD1 gene, responsible for OFDI subtype and coding for a centrosomal protein, has been known, suggesting the involvement of the primary cilium in OFDS. Mutations have recently been reported in the TMEM216, DDX59, SCLT1, TBC1D32 and TCTN3 genes in anecdotic cases. To identify new genes involved in OFDS, we performed whole-exome sequencing in 24 patients. In 14/24 cases, we identified 5 novel genes (C2CD3, TMEM107, INTU, KIAA0753, IFT57), enlarged the clini…
Genomic characterization of mosaic cutaneous pigmentary disorders
2019
Ntroduction: Mosaic cutaneous dyschromia is strongly evocative of an underlying genetic mosaicism. These post-zygotic events are challenging for conventional diagnostic tools. Thus, genetic basis of mosaic cutaneous dyschromia still remained poorly understood. Materials and Methods: The M.U.S.T.A.R.D. cohort gathers DNA from skin biopsies of patients with mosaic cutaneous dyschromia. After a specialised phenotype analysis, they are referred to either trio exome sequencing (ES) at 200X, or targeted ultra-deep sequencing (60,000X) of candidate genes. Data are analysed with a tailored pipeline, allowing detection of both low-rate nucleotidic variations or chromosomal events. Results: From 2013…
Microbial diversity and structure are drivers of the biological barrier effect against Listeria monocytogenes in soil
2013
International audience; Understanding the ecology of pathogenic organisms is important in order to monitor their transmission in the environment and the related health hazards. We investigated the relationship between soil microbial diversity and the barrier effect against Listeria monocytogenes invasion. By using a dilution-to-extinction approach, we analysed the consequence of eroding microbial diversity on L. monocytogenes population dynamics under standardised conditions of abiotic parameters and microbial abundance in soil microcosms. We demonstrated that highly diverse soil microbial communities act as a biological barrier against L. monocytogenes invasion and that phylogenetic compos…
Response of soil bacterial communities to the incorporation of crop residues : influence of agricultural practices and link with the soil biological …
2010
The effect of the location of wheat residues (soil surface vs. incorporated in soil) on their decomposition and on soil bacterial communities was investigated by the means of a field experiment. Bacterial-Automated Ribosomal Intergenic Spacer Analysis (B-ARISA) of DNA extracts from residues, detritusphere (soil adjacent to residues), and bulk soil evidenced that residues constitute the zone of maximal changes in bacterial composition. However, the location of the residues influenced greatly their decomposition and the dynamics of the colonizing bacterial communities. Sequencing of 16S rRNA gene in DNA extracts from the residues at the early, middle, and late stages of degradation confirmed …
Response of soil bacterial communities to the incorporation of crop residues : influence of agricultural practices and link with the soil biological …
2010
The effect of the location of wheat residues (soil surface vs. incorporated in soil) on their decomposition and on soil bacterial communities was investigated by the means of a field experiment. Bacterial-Automated Ribosomal Intergenic Spacer Analysis (B-ARISA) of DNA extracts from residues, detritusphere (soil adjacent to residues), and bulk soil evidenced that residues constitute the zone of maximal changes in bacterial composition. However, the location of the residues influenced greatly their decomposition and the dynamics of the colonizing bacterial communities. Sequencing of 16S rRNA gene in DNA extracts from the residues at the early, middle, and late stages of degradation confirmed …
Apport des nouvelles générations de séquençage pour accéder à la diversité des communautés microbiennes du sol : nécessité d’un ‘pipeline’ bio-inform…
2012
National audience
Utilisation et comparaison de méthodes de caractérisation moléculaire appliquées au genre Fusarium dans le cadre de la gestion de la collection de Mi…
2008
Affiche; Les champignons microscopiques du genre Fusarium (phylum des Ascomycètes) sontresponsables de nombreuses maladies sur plantes (blé, tomate, palmier …) engendrant de fortespertes de rendement. Ils sont par ailleurs responsables de la production de mycotoxines quipeuvent avoir un impact non négligeable en santé animale et humaine.